Vehicle management based on GPS/GIS: A case study on bulk concrete trucks

Vehicle management is a very important application area of GPS/GIS. Each customer has its special requirements on GIS system when he installs it. We discuss a common structure of our GPS/GIS implementation, and present a case study on bulk concrete trucks management for bulk concrete producers. With GPS/GIS system, bulk concrete producers can improve their performance in scheduling and management.

Vehicle management system-system analysis and design

This paper presents one of GPS Vehicle Monitor System on base of Web GIS technology,which is very important in vehicle tracking, scheduling and management. In the paper, a real GPS vehicle monitor system which using Internet technology is introduced. This system have been putted into practice successfully.

硅胶膜离心柱法能高效、可靠地提取黄秋葵的DNA

黄秋葵(Abelmoschus esculentus)组织中富含影响其DNA提取质量的多糖、多酚。为探究这一特性,本研究以黄秋葵幼叶为材料,探索了一种新的基因组DNA提取方法——硅胶膜离心柱法,利用硅胶膜离心柱与自配的溶液提取了高质量的DNA。此法先用不含十六烷基三甲基溴化铵(cetyl-trimethyl-ammonium bromide, CTAB)的缓冲液去除一部分次生代谢物;然后用3%CTAB缓冲液分离DNA;再用氯仿/异戊醇(24:1)抽提,之后用硅胶膜离心柱纯化DNA。运用琼脂糖凝胶电泳、ISSR-PCR扩增和限制性酶切等方法检测DNA的质量,并与传统CTAB法、十二烷基硫酸钠(sodium dodecyl sulfate, SDS)法和高盐低pH法进行比较分析。结果表明,本法提取的DNA完整性好,纯度高;能被Eco RⅠ完全消化;ISSR扩增得到的条带最清晰、多态性好,明显优于其它3种传统方法。此外,本方法也成功用于石斛(Dendrobium)、葡萄(Vitis spp.)、无花果(Ficus carica)、芦荟(Aloe vera)等植物基因组DNA的提取,因此具有广泛的适用性,可为富含多糖植物基因组DNA的提取提供参考。