Asymmetric somatic hybrid plants were pro-duced between cultivated rice (Oryza sativa L.) and wild spe-cies [O. meyeriana (Zoll. etMor. exSteud.)] with high resis-tance to rice bacterial blight. X-ray-irradiated proto...Asymmetric somatic hybrid plants were pro-duced between cultivated rice (Oryza sativa L.) and wild spe-cies [O. meyeriana (Zoll. etMor. exSteud.)] with high resis-tance to rice bacterial blight. X-ray-irradiated protoplasts of the wild species were used as donor and chemically fused with iodoacetamide-inactivated protoplasts of rice cv. 02428 to produce hybrids. Seventy-two plants were regenerated from 623 calli based on metabolic complementation. The morphological characters of the plants closely resembled that of the rice. Simple sequence repeats were employed to iden-tify their hybridity. Cytological analysis of root-tips revealed that their chromosome number varied in the range of 27— 38. The somatic hybrids were inoculated with strains of Xanthamonas oryzae pv. oryzae at adult growth stage and demonstrated the resistance to bacterial blight introgression from the O. meyeriana.展开更多
文摘Asymmetric somatic hybrid plants were pro-duced between cultivated rice (Oryza sativa L.) and wild spe-cies [O. meyeriana (Zoll. etMor. exSteud.)] with high resis-tance to rice bacterial blight. X-ray-irradiated protoplasts of the wild species were used as donor and chemically fused with iodoacetamide-inactivated protoplasts of rice cv. 02428 to produce hybrids. Seventy-two plants were regenerated from 623 calli based on metabolic complementation. The morphological characters of the plants closely resembled that of the rice. Simple sequence repeats were employed to iden-tify their hybridity. Cytological analysis of root-tips revealed that their chromosome number varied in the range of 27— 38. The somatic hybrids were inoculated with strains of Xanthamonas oryzae pv. oryzae at adult growth stage and demonstrated the resistance to bacterial blight introgression from the O. meyeriana.
