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基于UPLC-LTQ-Orbitrap-MS^(n)技术的红曲茯苓组方物质基础分析
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作者 李婷婷 梅凯 +7 位作者 李鑫 王灵敏 王昕怡 郝逗逗 张丽梅 宋建刚 刘葭 吴清 《中南药学》 CAS 2023年第3期657-666,共10页
目的采用超高效液相色谱-线性离子阱/静电场轨道阱组合式高分辨质谱联用(UPLC-LTQ-Orbitrap-MS^(n))技术对红曲茯苓组方中的化学成分进行定性鉴别,为其确定功效相关质量控制指标提供依据。方法采用Waters ACQUITY UPLC BHEC18(2.1 mm... 目的采用超高效液相色谱-线性离子阱/静电场轨道阱组合式高分辨质谱联用(UPLC-LTQ-Orbitrap-MS^(n))技术对红曲茯苓组方中的化学成分进行定性鉴别,为其确定功效相关质量控制指标提供依据。方法采用Waters ACQUITY UPLC BHEC18(2.1 mm×100 mm,1.7μm),以乙腈-0.1%甲酸水为流动相进行梯度洗脱,柱温35℃,流速0.3 mL·min^(-1),正、负离子模式进行扫描获得高分辨质谱信息。通过与对照品的保留时间及质谱数据信息对比,并结合相关文献报道对相应化合物的相对分子质量、质谱裂解规律进行匹配,对红曲茯苓组方中所含的成分群进行定性分析归类。结果共鉴定了84个化学成分,包括41个黄酮类、11个莫那可林类、10个红曲色素类、8个倍半萜类、4个氨基酸类、2个三萜类、3个多糖类以及5个有机酸类成分,并明确了各化学成分的来源归属以及结合文献报道确定功效相关成分。结论该研究准确地识别了红曲茯苓组方中的化学成分,可为其进一步开发及质量控制提供参考。 展开更多
关键词 红曲茯苓组方 UPLC-LTQ-Orbitrap-MS^(n) 化学成分
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Phosphorus Nutrient Characteristics of Different Maize (Zea mays L.) Inbreds for Tolerance to Low-P Stress 被引量:5
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作者 zhangli-mei HELi-yuan +1 位作者 LIJian-sheng XUShang-zhong 《Agricultural Sciences in China》 CAS CSCD 2005年第4期281-287,共7页
Phosphorus nutrient characteristics of different maize inbred lines to low-P stress were studied at stages of seedling, steming, earing, silking under pot culture. In the periods of seedling and steming, P uptake effi... Phosphorus nutrient characteristics of different maize inbred lines to low-P stress were studied at stages of seedling, steming, earing, silking under pot culture. In the periods of seedling and steming, P uptake efficiency was the main contributor to P tolerance, and the relative P content in P-tolerant genotypes, 99180 and 99239 were higher than that in sensitive genotype, 99152. At earing stage, P-tolerant genotypes, compared to P-sensitive ones, had higher accumulation of P in upper leaves. When came to the silking stage, P uptake and redistribution efficiency of P-tolerant genotypes were higher than those in 99152. The results also suggested that there are different mechanisms of P nutrient uptake and distribution in different P-tolerant genotypes. Inbred line 99239, according to the investigation, was considered as an efficient stock in the P-uptake while 99180 fallen to the efficient stock of P redistribution. 展开更多
关键词 磷素营养 玉米 低磷胁迫 品种 磷素吸收 磷素分配
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