目的检索汇总早中期帕金森病患者运动管理的相关证据,并总结最佳证据,为指导早中期帕金森病患者运动锻炼提供依据。方法确定循证问题,计算机检索UpToDate、JBI、BMJ、NGC、NICE、RNAO、GIN、Cochrane Library、PubMed、Web of Science、...目的检索汇总早中期帕金森病患者运动管理的相关证据,并总结最佳证据,为指导早中期帕金森病患者运动锻炼提供依据。方法确定循证问题,计算机检索UpToDate、JBI、BMJ、NGC、NICE、RNAO、GIN、Cochrane Library、PubMed、Web of Science、Embase、PEDro、中国知网、万方数据库、维普数据库、中国生物医学文献服务网及美国帕金森病协会、美国物理治疗协会网站,由2名研究员对文献质量进行评价,并对符合质量标准的文献进行证据提取。结果共纳入18篇文献,其中指南4篇、专家意见2篇、系统评价11篇、质性系统评价1篇。分析总结后提取了包括运动时机、运动评估与测试、运动实施、运动安全、健康教育、效果评价6个方面的29条项目。结论本研究总结早中期帕金森病患者运动管理的相关证据,运动可改善帕金森的症状,医护人员需依据评估结果,参考患者的实际情况,进行个性化运动指导,以期延缓疾病进展,提高生活质量。展开更多
Objective To investigate the effects of matrine on antigen presentation of dendritic cells(DCs),and to explore the pharmacological mechanism of matrine on anti-tumor effect.Methods Different concentrations(0,1,2,4,8 a...Objective To investigate the effects of matrine on antigen presentation of dendritic cells(DCs),and to explore the pharmacological mechanism of matrine on anti-tumor effect.Methods Different concentrations(0,1,2,4,8 and 16µg/mL)of matrine were co-cultured with DCs,the harvested DCs were co-cultured with antigens of Lewis lung cancer(LLC)cells,and then DCs and T cells were co-cultured to produce DCs-activated killer(DAK)cells,which have significant tumor-killing activity.The expression of cytokines,mRNA and protein of toll-like receptors(TLRs)in DCs were detected by enzyme linked immunosobent assay,polymerase chain reaction and Western blot,respectively.And the killing effect of DAK were measured by MTT assay.Results Matrine significantly increased the mRNA expression of TLR7,TLR8,myeloid differentiation factor 88(MyD88),tumor necrosis factor receptor-associated factor 6(TRAF-6)and IκB kinase(IKK),as well as the protein expression of TLR7 and TLR8,and up-regulated the levels of interleukin-12(IL-12),IL-6 and tumor necrosis factor-α(TNF-α),meanwhile,it also increased the expressions of MHC-II,CD54,CD80 and CD86 in DCs.DCs-activated effector T cells had significant tumor-killing activity.When the concentration of matrine was more than 4µg/mL,all indices had significant difference(P<0.01 or P<0.05).Conclusion Matrine plays an anti-tumor role by regulating TLRs signal transduction pathway,promoting the secretion of inflammatory cytokines and enhancing immune function.展开更多
文摘目的检索汇总早中期帕金森病患者运动管理的相关证据,并总结最佳证据,为指导早中期帕金森病患者运动锻炼提供依据。方法确定循证问题,计算机检索UpToDate、JBI、BMJ、NGC、NICE、RNAO、GIN、Cochrane Library、PubMed、Web of Science、Embase、PEDro、中国知网、万方数据库、维普数据库、中国生物医学文献服务网及美国帕金森病协会、美国物理治疗协会网站,由2名研究员对文献质量进行评价,并对符合质量标准的文献进行证据提取。结果共纳入18篇文献,其中指南4篇、专家意见2篇、系统评价11篇、质性系统评价1篇。分析总结后提取了包括运动时机、运动评估与测试、运动实施、运动安全、健康教育、效果评价6个方面的29条项目。结论本研究总结早中期帕金森病患者运动管理的相关证据,运动可改善帕金森的症状,医护人员需依据评估结果,参考患者的实际情况,进行个性化运动指导,以期延缓疾病进展,提高生活质量。
基金Supported by the National Natural Science Foundation of China(Nos.81773960 and 81973535)。
文摘Objective To investigate the effects of matrine on antigen presentation of dendritic cells(DCs),and to explore the pharmacological mechanism of matrine on anti-tumor effect.Methods Different concentrations(0,1,2,4,8 and 16µg/mL)of matrine were co-cultured with DCs,the harvested DCs were co-cultured with antigens of Lewis lung cancer(LLC)cells,and then DCs and T cells were co-cultured to produce DCs-activated killer(DAK)cells,which have significant tumor-killing activity.The expression of cytokines,mRNA and protein of toll-like receptors(TLRs)in DCs were detected by enzyme linked immunosobent assay,polymerase chain reaction and Western blot,respectively.And the killing effect of DAK were measured by MTT assay.Results Matrine significantly increased the mRNA expression of TLR7,TLR8,myeloid differentiation factor 88(MyD88),tumor necrosis factor receptor-associated factor 6(TRAF-6)and IκB kinase(IKK),as well as the protein expression of TLR7 and TLR8,and up-regulated the levels of interleukin-12(IL-12),IL-6 and tumor necrosis factor-α(TNF-α),meanwhile,it also increased the expressions of MHC-II,CD54,CD80 and CD86 in DCs.DCs-activated effector T cells had significant tumor-killing activity.When the concentration of matrine was more than 4µg/mL,all indices had significant difference(P<0.01 or P<0.05).Conclusion Matrine plays an anti-tumor role by regulating TLRs signal transduction pathway,promoting the secretion of inflammatory cytokines and enhancing immune function.
