We assessed the in vitro cytotoxicity of Mg-6Zn alloy and analyzed the cell apoptosis rate and the expression of caspase-3 to evaluate the effects of Mg-6Zn alloy extracts on apoptosis of intestinal epithelial cells（...We assessed the in vitro cytotoxicity of Mg-6Zn alloy and analyzed the cell apoptosis rate and the expression of caspase-3 to evaluate the effects of Mg-6Zn alloy extracts on apoptosis of intestinal epithelial cells（IEC）-6. IEC-6 cells were cultured in different concentrations of Mg-6Zn alloy extracts（40%, 20%） and in the control group. The indirect effects of Mg-6Zn alloy on IEC-6 cells were studied by calculating the cell relative growth rate（RGR）, measuring the apoptosis of IEC-6 cells through flow cytometry, and investigating the expression of caspase-3 using real-time polymerase chain reaction. The experimental results show that the cytotoxicity of these extracts is Grade 0-1. The level of apoptosis in IEC-6 cells cultured in 40% Mg-6Zn alloy extracts is significantly higher than that in cells treated with 20% extract and the control group. The expression of caspase-3 is found to be up-regulated in the 40% extract as compared to 20% extract and the control group. Taken together, the data show that the Mg-6Zn alloy in 40% and 20% concentration extracts proves noncytotoxicity. But the 40% concentration of Mg-6Zn alloy extract can induce the apoptosis and the related caspase-3 expression in vitro.展开更多
A magnesium-zinc alloy rod was implanted into the marrow cavity of the distal femur in New Zealand rabbits. The femur with the implanted alloy was compared with the contralateral femur in which a bone tunnel without i...A magnesium-zinc alloy rod was implanted into the marrow cavity of the distal femur in New Zealand rabbits. The femur with the implanted alloy was compared with the contralateral femur in which a bone tunnel without implant was formed as a control. Degradation of the magnesium-zinc alloy was analyzed via X-ray, scanning electron microscopy, and element energy spectrum analysis. Serum magnesium, liver and kidney function tests, and myocardial enzymes were measured. Heart, liver, kidney and spleen were sectioned for pathological analysis, and the effects of the implanted material on the histology and function of important organs were analyzed. Magnesium-zinc alloy was resorbed from the bone marrow cavity of the femur; 87% of the alloy was degraded within 14 weeks after the surgery. There were no significant differences in serum magnesium, liver or kidney function tests, or myocardial enzymes be- fore the surgery and after degradation of the magnesium-zinc alloy. Histology of the heart, liver, kidney, and spleen did not change. This study demonstrated that magnesium-zinc alloy can be resorbed in bone, and that the degradation products have good biocompatibility with heart, liver, kidney, and spleen.展开更多
The objective of the present study was to prepare a novel nanostructured surface of Ti6Al4V alloy by the severe plastic deformation (SPD) and the chemical treatment process and to evaluate the adhesion of osteoblast o...The objective of the present study was to prepare a novel nanostructured surface of Ti6Al4V alloy by the severe plastic deformation (SPD) and the chemical treatment process and to evaluate the adhesion of osteoblast on the nanophase titanium alloy. In the in vitro study, the primary cultured osteoblasts of neonatal rat calvaria were cultured on the nanophase and the as-received smooth Ti6Al4V substrates. Then osteoblasts adhesion behaviors on different substrates were observed by the fluorescence mi-croscopy, scanning electron microscopy (SEM) and RT-PCR analysis. The results of our research showed increased osteoblast adhesion on the nanophase titanium alloy compared with the as-received case. On the nanophase substrate, the presence of extensive filopodia, strong cellular adhesion and early cellular confluency could be observed. In addition, the expression of the adhesion-related integrin β1 mRNA was also higher on the nanophase substrate. It suggested that the nano technology could be further considered for orthopedic implant applications.展开更多
In order to manage the impaired wound healing of diabetic chronic wounds induced by high-glucose-damaged endothelial cells,a glucose-induced injured endothelial cell model is established in this research to explore th...In order to manage the impaired wound healing of diabetic chronic wounds induced by high-glucose-damaged endothelial cells,a glucose-induced injured endothelial cell model is established in this research to explore the regulatory effects of a supramolecular chiral hydrogel on injured endothelial cells.Cellular behaviors of endothelial cells under different culture conditions are evaluated by CCK-8,5-ethynyl-2′-deoxyuridine(EdU)staining,adhesion,and Transwell assays.The expression levels of angiogenesis-related markers,including nitric ox-ide,endothelial nitric oxide synthase(eNOS),and vascular endothelial growth factor(VEGF),are also assessed by enzyme-linked immune sorbent assay(ELISA)and the polymerase chain reaction(PCR).The results demonstrate that the left-handed chirality of the supramolecular chiral hydrogel can promote cell proliferation and enhance the adhesion and migration ability of impaired endothelial cells.Moreover,enhanced nitric oxide synthesis and elevated expression of eNOS and VEGF are observed in cells in the left-handed chiral environment.Thus,the left-handed supramolecular chiral hydrogel can regulate the cellular behavior of high-glucose-injured endothelial cells,including cell proliferation,adhesion,migration,and angiogenesis,offering the potential to promote diabetic wound healing.展开更多
基金Funded by the National Natural Science Foundation of China(Nos.30901422&51271117)the Shanghai Jiao Tong University Interdisciplinary(Biomedical Engineering)Research Fund(No.YG2010MS45)the Shanghai Jiao Tong University School of Medicine Science and Technology Fund(No.09XJ21005)
文摘We assessed the in vitro cytotoxicity of Mg-6Zn alloy and analyzed the cell apoptosis rate and the expression of caspase-3 to evaluate the effects of Mg-6Zn alloy extracts on apoptosis of intestinal epithelial cells（IEC）-6. IEC-6 cells were cultured in different concentrations of Mg-6Zn alloy extracts（40%, 20%） and in the control group. The indirect effects of Mg-6Zn alloy on IEC-6 cells were studied by calculating the cell relative growth rate（RGR）, measuring the apoptosis of IEC-6 cells through flow cytometry, and investigating the expression of caspase-3 using real-time polymerase chain reaction. The experimental results show that the cytotoxicity of these extracts is Grade 0-1. The level of apoptosis in IEC-6 cells cultured in 40% Mg-6Zn alloy extracts is significantly higher than that in cells treated with 20% extract and the control group. The expression of caspase-3 is found to be up-regulated in the 40% extract as compared to 20% extract and the control group. Taken together, the data show that the Mg-6Zn alloy in 40% and 20% concentration extracts proves noncytotoxicity. But the 40% concentration of Mg-6Zn alloy extract can induce the apoptosis and the related caspase-3 expression in vitro.
基金Supported by the National Natural Science Foundation of China (Grant No. 30772182)the Medical-Industial intersect study in Shanghai Jiaotong University (Grant No. YG2007MS26)
文摘A magnesium-zinc alloy rod was implanted into the marrow cavity of the distal femur in New Zealand rabbits. The femur with the implanted alloy was compared with the contralateral femur in which a bone tunnel without implant was formed as a control. Degradation of the magnesium-zinc alloy was analyzed via X-ray, scanning electron microscopy, and element energy spectrum analysis. Serum magnesium, liver and kidney function tests, and myocardial enzymes were measured. Heart, liver, kidney and spleen were sectioned for pathological analysis, and the effects of the implanted material on the histology and function of important organs were analyzed. Magnesium-zinc alloy was resorbed from the bone marrow cavity of the femur; 87% of the alloy was degraded within 14 weeks after the surgery. There were no significant differences in serum magnesium, liver or kidney function tests, or myocardial enzymes be- fore the surgery and after degradation of the magnesium-zinc alloy. Histology of the heart, liver, kidney, and spleen did not change. This study demonstrated that magnesium-zinc alloy can be resorbed in bone, and that the degradation products have good biocompatibility with heart, liver, kidney, and spleen.
文摘The objective of the present study was to prepare a novel nanostructured surface of Ti6Al4V alloy by the severe plastic deformation (SPD) and the chemical treatment process and to evaluate the adhesion of osteoblast on the nanophase titanium alloy. In the in vitro study, the primary cultured osteoblasts of neonatal rat calvaria were cultured on the nanophase and the as-received smooth Ti6Al4V substrates. Then osteoblasts adhesion behaviors on different substrates were observed by the fluorescence mi-croscopy, scanning electron microscopy (SEM) and RT-PCR analysis. The results of our research showed increased osteoblast adhesion on the nanophase titanium alloy compared with the as-received case. On the nanophase substrate, the presence of extensive filopodia, strong cellular adhesion and early cellular confluency could be observed. In addition, the expression of the adhesion-related integrin β1 mRNA was also higher on the nanophase substrate. It suggested that the nano technology could be further considered for orthopedic implant applications.
基金the National Natural Science Foundation of China(No.81902210)the Transnational Medicine Foundation of Shanghai Jiao Tong University(No.ZH2018QNA12)。
文摘In order to manage the impaired wound healing of diabetic chronic wounds induced by high-glucose-damaged endothelial cells,a glucose-induced injured endothelial cell model is established in this research to explore the regulatory effects of a supramolecular chiral hydrogel on injured endothelial cells.Cellular behaviors of endothelial cells under different culture conditions are evaluated by CCK-8,5-ethynyl-2′-deoxyuridine(EdU)staining,adhesion,and Transwell assays.The expression levels of angiogenesis-related markers,including nitric ox-ide,endothelial nitric oxide synthase(eNOS),and vascular endothelial growth factor(VEGF),are also assessed by enzyme-linked immune sorbent assay(ELISA)and the polymerase chain reaction(PCR).The results demonstrate that the left-handed chirality of the supramolecular chiral hydrogel can promote cell proliferation and enhance the adhesion and migration ability of impaired endothelial cells.Moreover,enhanced nitric oxide synthesis and elevated expression of eNOS and VEGF are observed in cells in the left-handed chiral environment.Thus,the left-handed supramolecular chiral hydrogel can regulate the cellular behavior of high-glucose-injured endothelial cells,including cell proliferation,adhesion,migration,and angiogenesis,offering the potential to promote diabetic wound healing.
