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原花青素对2型糖尿病局灶性脑缺血大鼠脑组织半胱氨酸蛋白酶3表达的影响 被引量:2
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作者 宋程光 闵鹤鸣 +2 位作者 杨鑫 赵传胜 闵连秋 《中国基层医药》 CAS 2019年第6期693-696,共4页
目的研究原花青素(Procyanidin,PC)对2型糖尿病局灶性脑缺血大鼠脑组织半胱氨酸蛋白酶3表达的影响.方法遵循随机原则,将40只健康SD(Sprague-Dawley,SD)大鼠按顺序编号,分为正常大鼠脑缺血组、2型糖尿病脑缺血组、原花青素三个剂量组(原... 目的研究原花青素(Procyanidin,PC)对2型糖尿病局灶性脑缺血大鼠脑组织半胱氨酸蛋白酶3表达的影响.方法遵循随机原则,将40只健康SD(Sprague-Dawley,SD)大鼠按顺序编号,分为正常大鼠脑缺血组、2型糖尿病脑缺血组、原花青素三个剂量组(原花青素-1、2、3组,分别予原花青素粉剂50 mg·kg^-1·次^-1、100 mg·kg^-1·次^-1、200 mg·kg^-1·次^-1),每组8只大鼠.制作2型糖尿病局灶性脑缺血大鼠模型,免疫组化SABC染色法检测半胱氨酸蛋白酶3(cysteine proteinase-3,Caspase-3)的表达,计数大鼠缺血侧大脑皮质区每高倍镜视野Caspase-3蛋白的阳性细胞数.结果与正常大鼠脑缺血组的(11.42±2.52)个比较,2型糖尿病脑缺血组大鼠脑组织Caspase-3阳性细胞数(15.00±2.38)个(t=2.17,P<0.01);与2型糖尿病脑缺血组比较:原花青素-1、2、3组均能不同程度减少,Caspase-3蛋白表达(9.38±2.00)个,(7.71±1.55)个,(6.96±1.57)个(t=2.86、3.13、3.36,均P<0.01),其中原花青素-2、3组较原花青素-1组下降更明显(t=1.92、2.03,P<0.01),但原花青素-2、3组间差异无统计学意义(t=1.13,P>0.05).结论 2型糖尿病局灶性脑缺血大鼠Caspase-3蛋白表达明显增加,原花青素能降低Caspase-3蛋白的表达,从而可能抑制细胞凋亡. 展开更多
关键词 缺氧缺血 糖尿病 2型 原花青素类 半胱氨酸蛋白水解酶类 细胞凋亡 自由基 再灌注损伤 大鼠 Sprague-dawley
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Mining Compressed Frequent Subtrees Set
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作者 zhao chuanshen WANG Xianyong +1 位作者 SUN Zhihui LI Yuetian 《Wuhan University Journal of Natural Sciences》 CAS 2009年第1期29-34,共6页
The number of frequent subtrees usually grows exponentially with the tree size because of combinatorial explosion. As a result, there are too many frequent subtrees for users to manage and use. To solve this problem, ... The number of frequent subtrees usually grows exponentially with the tree size because of combinatorial explosion. As a result, there are too many frequent subtrees for users to manage and use. To solve this problem, we generalize a compressed frame based on δ-cluster to the problem of compressing frequent-subtree sets, and propose an algorithm RPTlocal which can mine compressed frequent subtrees set directly. This algorithm sacrifices the theoretical bounds but still has good compression quality. By pruning the search space and generating frequent subtrees directly, this algorithm is also efficient. Experiment result shows that the representative subtrees mining by RPTlocal is almost two orders of magnitude less than the whole collection of the closed subtrees, and is more efficient than CMtreeMiner, the algorithm for mining both closed and Maximal frequent subtrees. 展开更多
关键词 data mining frequent subtrees projected branch
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