Cadmium zinc telluride(CZT)is a preferred material for X-ray and gamma-ray detector.Thanks to the relatively high atomic number,high density and wide band-gap,CZT detector possesses sharp energy resolution and high de...Cadmium zinc telluride(CZT)is a preferred material for X-ray and gamma-ray detector.Thanks to the relatively high atomic number,high density and wide band-gap,CZT detector possesses sharp energy resolution and high detection efficiency without cryogenic cooling.We have developed a CdZnTe detector with an energy resolution of 3.45%(FWHM)at 59.54 keV at room temperature,and it is used for X-ray fluorescence analysis.In this paper, leakage current,energy resolution and long term stability of the CZT detector are discussed.展开更多
热不对称交错PCR(TAIL-PCR)是检测转基因作物目的基因插入位点的主要技术。为探索适宜大豆的TAIL-PCR反应体系,采用单因素试验和L16(45)正交试验对影响转基因大豆TAIL-PCR的主要参数进行分析,以期建立成熟的大豆TAIL-PCR技术体系。试验...热不对称交错PCR(TAIL-PCR)是检测转基因作物目的基因插入位点的主要技术。为探索适宜大豆的TAIL-PCR反应体系,采用单因素试验和L16(45)正交试验对影响转基因大豆TAIL-PCR的主要参数进行分析,以期建立成熟的大豆TAIL-PCR技术体系。试验设计3个嵌套特异性引物和5种兼并引物进行了3次巢式的热不对称PCR反应。结果表明:退火温度、酶用量、模板浓度、兼并引物和较低温特异性循环在不同水平对TAIL-PCR反应结果均有影响,优化的TAIL-PCR反应体系,即第2次反应为退火温度61℃,1.0 U/30 m L Taq酶,模板浓度30 ng/μL;第3次反应为退火温度62℃,0.5 U/50m L Taq酶,模板浓度40 ng/μL,反应过程中采用降低5个较低温特异性循环,兼并引物采用AD3,在此条件下扩增的条带清晰、稳定、特异性好,适合大豆TAIL-PCR反应体系。展开更多
文摘Cadmium zinc telluride(CZT)is a preferred material for X-ray and gamma-ray detector.Thanks to the relatively high atomic number,high density and wide band-gap,CZT detector possesses sharp energy resolution and high detection efficiency without cryogenic cooling.We have developed a CdZnTe detector with an energy resolution of 3.45%(FWHM)at 59.54 keV at room temperature,and it is used for X-ray fluorescence analysis.In this paper, leakage current,energy resolution and long term stability of the CZT detector are discussed.
文摘热不对称交错PCR(TAIL-PCR)是检测转基因作物目的基因插入位点的主要技术。为探索适宜大豆的TAIL-PCR反应体系,采用单因素试验和L16(45)正交试验对影响转基因大豆TAIL-PCR的主要参数进行分析,以期建立成熟的大豆TAIL-PCR技术体系。试验设计3个嵌套特异性引物和5种兼并引物进行了3次巢式的热不对称PCR反应。结果表明:退火温度、酶用量、模板浓度、兼并引物和较低温特异性循环在不同水平对TAIL-PCR反应结果均有影响,优化的TAIL-PCR反应体系,即第2次反应为退火温度61℃,1.0 U/30 m L Taq酶,模板浓度30 ng/μL;第3次反应为退火温度62℃,0.5 U/50m L Taq酶,模板浓度40 ng/μL,反应过程中采用降低5个较低温特异性循环,兼并引物采用AD3,在此条件下扩增的条带清晰、稳定、特异性好,适合大豆TAIL-PCR反应体系。