Africanswinefever(ASF),causedbythe African swine fever virus (ASFV), is an acute, hemorrhagic, and contagious disease of domestic pigs and wild boars.The disease is notifiable and listed by the World Organization for ...Africanswinefever(ASF),causedbythe African swine fever virus (ASFV), is an acute, hemorrhagic, and contagious disease of domestic pigs and wild boars.The disease is notifiable and listed by the World Organization for Animal Health (WOAH)(Wang N et al. 2019).展开更多
African swine fever(ASF) was first discovered and reported in East Africa in 1921 and it has been listed as a notifiable animal disease by the World Organization for Animal Health(WOAH) due to the high morbidity and m...African swine fever(ASF) was first discovered and reported in East Africa in 1921 and it has been listed as a notifiable animal disease by the World Organization for Animal Health(WOAH) due to the high morbidity and mortality(WOAH 2019). African swine fever virus(ASFV) can persist in pig farm environments, carcasses, and various pig products(Petrini et al. 2019). ASF has caused significant economic losses to the pig industry since its outbreak in China in 2018(Zhao et al. 2019).展开更多
Prevention of African swine fever,a disease caused by African swine fever virus(ASFV),requires maintenance of high biosecurity standards,which principally relies on disinfection.Finding the perfect disinfectant agains...Prevention of African swine fever,a disease caused by African swine fever virus(ASFV),requires maintenance of high biosecurity standards,which principally relies on disinfection.Finding the perfect disinfectant against ASFV is difficult because of the lack of relevant data.Therefore,we aimed to find the most effective disinfectant and to optimise its concentration as well as contact time to confirm the viricidal effect against ASFV in vitro.We evaluated the viricidal activity of three concentrations each of six common disinfectants against ASFV using immersion disinfection assay(IDA)and spray disinfection assay(SDA);the concentrations of these disinfectants at which complete viral inactivation occurred were almost same as the manufacturer-recommended concentrations,but the exposure times for viral inactivation are different.The following disinfectants(assay:concentration,exposure time)showed complete inactivation:iodine and acid mixed solution(IDA/SDA:0.5%,10 min);compound potassium peroxymonosulfate(IDA:0.25%,30 min;SDA:0.25%,60 min);citric acid(IDA:0.25%,60 min;SDA:0.5%,60 min);sodium dichloroisocyanurate(IDA:0.125%,60 min;SDA:0.25%,60 min);and glutaral ang deciquam(IDA/SDA:0.2%,60 min);and deciquam(IDA/SDA:0.5%,60 min).However,in the presence of organic material contamination,disinfectants did not show a marked inactivation effect.Therefore,disinfection procedures should be performed in two steps:thorough mechanical cleaning followed by application of disinfectant.In conclusion,all the tested disinfectants can inactivate ASFV;these can be used as alternative disinfectants to enhance biosecurity.展开更多
基金supported by the National Key R&D Program of China (2021 YF D1800101 and 2019YFE0107300)the Applied Technology Research and Development Project of Heilongjiang Province, China (GA19B301)the Central Public-Interest Scientific Institution Basal Research Fund, China (1610302022003)。
文摘Africanswinefever(ASF),causedbythe African swine fever virus (ASFV), is an acute, hemorrhagic, and contagious disease of domestic pigs and wild boars.The disease is notifiable and listed by the World Organization for Animal Health (WOAH)(Wang N et al. 2019).
基金supported by the National Key Research and Development Program of China (2021YFC2600305 and 2021YFD1801405)。
文摘African swine fever(ASF) was first discovered and reported in East Africa in 1921 and it has been listed as a notifiable animal disease by the World Organization for Animal Health(WOAH) due to the high morbidity and mortality(WOAH 2019). African swine fever virus(ASFV) can persist in pig farm environments, carcasses, and various pig products(Petrini et al. 2019). ASF has caused significant economic losses to the pig industry since its outbreak in China in 2018(Zhao et al. 2019).
基金supported by the National Key R&D Program of China(2018YFC1200600)the grant from the State Key Laboratory of Veterinary Biotechnology Program(SKLVBP201801)the National Science and Technology Major Project of China(2018ZX10734401-018-002)。
文摘Prevention of African swine fever,a disease caused by African swine fever virus(ASFV),requires maintenance of high biosecurity standards,which principally relies on disinfection.Finding the perfect disinfectant against ASFV is difficult because of the lack of relevant data.Therefore,we aimed to find the most effective disinfectant and to optimise its concentration as well as contact time to confirm the viricidal effect against ASFV in vitro.We evaluated the viricidal activity of three concentrations each of six common disinfectants against ASFV using immersion disinfection assay(IDA)and spray disinfection assay(SDA);the concentrations of these disinfectants at which complete viral inactivation occurred were almost same as the manufacturer-recommended concentrations,but the exposure times for viral inactivation are different.The following disinfectants(assay:concentration,exposure time)showed complete inactivation:iodine and acid mixed solution(IDA/SDA:0.5%,10 min);compound potassium peroxymonosulfate(IDA:0.25%,30 min;SDA:0.25%,60 min);citric acid(IDA:0.25%,60 min;SDA:0.5%,60 min);sodium dichloroisocyanurate(IDA:0.125%,60 min;SDA:0.25%,60 min);and glutaral ang deciquam(IDA/SDA:0.2%,60 min);and deciquam(IDA/SDA:0.5%,60 min).However,in the presence of organic material contamination,disinfectants did not show a marked inactivation effect.Therefore,disinfection procedures should be performed in two steps:thorough mechanical cleaning followed by application of disinfectant.In conclusion,all the tested disinfectants can inactivate ASFV;these can be used as alternative disinfectants to enhance biosecurity.
