The hepatopancreatic necrosis disease(HPND)is a common shrimp disease.Aquaculture of the black tiger shrimp Penaeus monodon has suffered with the frequent outbreak of HPND.Understanding the molecular mechanisms of hos...The hepatopancreatic necrosis disease(HPND)is a common shrimp disease.Aquaculture of the black tiger shrimp Penaeus monodon has suffered with the frequent outbreak of HPND.Understanding the molecular mechanisms of host response to HPND is of pivotal importance for improving its farming performance.In this study,the RNA-Seq platform was utilized to investigate transcriptomic changes of the hepatopancreas in pond-cultured P.monodon with HPND symptoms.A total of 62071 genes with an average length of 953 bp were obtained,and a lot of simple sequence repeat(SSR)loci related to these genes were identified.Totally,5204 differentially expressed genes(DEGs)were detected between healthy shrimp and HPND shrimp,with 3399 genes upregulated and 1,805 genes down-regulated.These genes had a wide range of biological functions,and several well-known immunerelated genes including integrin alpha 5,integrin beta 1,C type lectin and catalase were among the DEGs.Cell signaling pathways including the extracellular matrix(ECM)receptor interaction,phagosome and lysosome pathways were significantly upregulated in HPND-infected shrimps,indicating their involvement in the immune responses of shrimp against HPND.The data obtained in this study offers new insights into the molecular mechanisms of shrimp host response to HPND disease,and provides a resource for molecular marker development and genetic breeding studies of P.monodon.展开更多
文摘海参幼苗发育需要经历耳状幼体、樽形幼体、五触手幼体及幼苗阶段,而从浮游幼体变态发育至附着幼苗阶段的高死亡率是热带海参繁育中的共性问题,目前有关热带海参变态发育的调控机制仍不清楚。以玉足海参(Holothuria leucospilota)为研究对象,采集小耳幼体(A)、中耳幼体(B)、大耳幼体(C)和樽形幼体(D)4个时期样品进行高通量转录组测序分析,以探究其变态发育的分子机制。结果显示,共产生83.6 GB Raw reads,拼接获得93528个Unigenes。对4个组测序文库的相邻组间(A_vs_B,B_vs_C,C_vs_D)进行两两比较,A_vs_B、B_vs_C和C_vs_D的差异表达基因数目分别为17732、11757和11319个。GO功能富集显示,差异基因主要富集于分子功能、催化活性等与细胞成长相关的GO功能。此外对KEGG通路进行了分析,结果显示差异基因显著富集于PI3K-Akt、细胞周期、癌症途径等与细胞分化增殖、凋亡相关的信号通路中,其中在幼体由浮游的大耳状幼体变态至附着的樽形幼体过程中,癌症途径的富集频率显著上升,表明其在幼体生长发育模式转换上发挥了关键作用。筛选的差异表达基因及预测的功能信息可为热带海参生长发育调控机制研究、人工繁育及分子改良应用提供参考。
基金This study was funded in part by the Natural Science Foundation of Guangdong Province(No.2018A030310049)2019 Annual Guangdong Provincial Special fund of Nan-hai Economic Shrimp Breeding and Culture Laboratory(No.2319412525)+2 种基金the Nanhai Scholar Project of Guangdong Ocean University,Fangchenggang Science and Technol-ogy Plan Project(No.AD19008017)the R&D Program of Key Areas in Guangdong Province(No.2020B0202010009)2019 Provincial Financial Special Fund Con-struction Project of Guangdong Ocean University(No.231619003).
文摘The hepatopancreatic necrosis disease(HPND)is a common shrimp disease.Aquaculture of the black tiger shrimp Penaeus monodon has suffered with the frequent outbreak of HPND.Understanding the molecular mechanisms of host response to HPND is of pivotal importance for improving its farming performance.In this study,the RNA-Seq platform was utilized to investigate transcriptomic changes of the hepatopancreas in pond-cultured P.monodon with HPND symptoms.A total of 62071 genes with an average length of 953 bp were obtained,and a lot of simple sequence repeat(SSR)loci related to these genes were identified.Totally,5204 differentially expressed genes(DEGs)were detected between healthy shrimp and HPND shrimp,with 3399 genes upregulated and 1,805 genes down-regulated.These genes had a wide range of biological functions,and several well-known immunerelated genes including integrin alpha 5,integrin beta 1,C type lectin and catalase were among the DEGs.Cell signaling pathways including the extracellular matrix(ECM)receptor interaction,phagosome and lysosome pathways were significantly upregulated in HPND-infected shrimps,indicating their involvement in the immune responses of shrimp against HPND.The data obtained in this study offers new insights into the molecular mechanisms of shrimp host response to HPND disease,and provides a resource for molecular marker development and genetic breeding studies of P.monodon.
