以聚乳酸/羟基乙酸(PLGA)共聚物为药物装载材料、油酸改性的四氧化三铁(Fe 3 O 4@OA)纳米颗粒为磁性功能材料,制备姜黄素/PLGA磁性微球,考察了乳化方法、高速均质速度、水油相体积比、PLGA用量、水相聚乙烯醇(PVA)质量分数、Fe 3 O 4@O...以聚乳酸/羟基乙酸(PLGA)共聚物为药物装载材料、油酸改性的四氧化三铁(Fe 3 O 4@OA)纳米颗粒为磁性功能材料,制备姜黄素/PLGA磁性微球,考察了乳化方法、高速均质速度、水油相体积比、PLGA用量、水相聚乙烯醇(PVA)质量分数、Fe 3 O 4@OA用量和姜黄素用量对磁性微球粒径的影响,并通过光学显微镜、激光粒度仪、紫外分光光度计、振动样品磁强计、热重分析仪等对其形貌及理化性能进行分析。研究结果显示:姜黄素/PLGA磁性微球的最佳制备工艺为选用搅拌-均质两步乳化法、高速均质15000 r/min、水油相体积比10∶1、PLGA用量100 mg、水相PVA质量分数1%、Fe 3 O 4@OA用量40 mg和姜黄素用量40 mg,此条件下制备的微球表面光滑,粒径较均一,多分散性指数(PDI)为0.38,平均粒径为3.60μm,微球的磁感应强度为14.12(A·m^(2))/kg,含磁量约为27.98%。载药微球的包封率为97.09%,载药率为6.40%,在体外释放实验中表现出明显的缓释效果。展开更多
This paper presents a systematic study of major and trace elements and Sm-Nd isotopes in leucogranites closely related to uranium mineralization in the Gaudeanmus area, Namibia. The results illustrate that the uranife...This paper presents a systematic study of major and trace elements and Sm-Nd isotopes in leucogranites closely related to uranium mineralization in the Gaudeanmus area, Namibia. The results illustrate that the uraniferous leucogranites possess high SiO2 （68.8wt%-76.0wt%, average 73.1wt%） and K （4.05wt%-7.78wt%, average 5.94wt%） contents, and are sub-alkaline and metaluminous to weakly peraluminous, as reflected by A/CNK values of 0.96-1.07 with an average of 1.01. The leucogranites are rich in light rare earth elements （LREE/HREE = 2.53-7.71; （La/Yb）s = 2.14-10.40）, have moderate Eu depletion and high Rb/Sr ratios （2.03-5.50 with an average of 4.36）; meanwhile, they are enriched in Rb, K, Th, U and Pb, and depleted in Ba, Nb, Ta, and Sr. The tNd（t） values of uraninites range from -14.8 to -16.5, and the two-stage Nd model ages are 2.43-2.56 Ga. Detailed elemental and Sm-Nd isotopic geochemical characteristics suggest that the leucogranites were formed in a post- orogenic extensional environment. The U-rich pre-Damara basement was the main source of uranium during the primary mineralization event, which is disseminated in leucogranites, whereas the uranium mineralization in veins possibly resulted from remobilization of the primary uranium minerals.展开更多
目的:研究分析可溶性内皮细胞蛋白C受体(Endothelial cell protein C receptor,sEPCR)与膜型EPCR(mEPCR)在参与类风湿关节炎(Rheumatoid Arthritis,RA)疾病进展中的作用。方法:在DBA1/J小鼠尾部注射II型胶原蛋白诱导关节炎小鼠(CIA小鼠)...目的:研究分析可溶性内皮细胞蛋白C受体(Endothelial cell protein C receptor,sEPCR)与膜型EPCR(mEPCR)在参与类风湿关节炎(Rheumatoid Arthritis,RA)疾病进展中的作用。方法:在DBA1/J小鼠尾部注射II型胶原蛋白诱导关节炎小鼠(CIA小鼠),并在两次胶原免疫期间给予实验组小鼠腹腔内注射sEPCR重组蛋白或EPCR腺病毒小干扰载体进行发病前干预;二次加强免疫后的第7 d,对CIA小鼠的关节红肿程度进行连续评分;第28 d处死小鼠,进一步行关节的组织病理学、放射学评估,评价sEPCR和mEPCR对RA疾病进展的作用。结果:(1)与对照组相比,给予sEPCR干预的CIA小鼠关节评分显著降低,组织学和放射学结果显示骨侵蚀程度也较轻;(2)与sEPCR重组蛋白干预相反,mEPCR组CIA小鼠由于EPCR表达下调,CIA小鼠表现为更严重的关节破坏和炎症。结论:EPCR的表达异常与RA的疾病进展密切相关;给予sEPCR治疗可延缓骨破坏,缓解RA的疾病进展;而mEPCR的表达水平降低会介导RA疾病的发生和发展。展开更多
文摘以聚乳酸/羟基乙酸(PLGA)共聚物为药物装载材料、油酸改性的四氧化三铁(Fe 3 O 4@OA)纳米颗粒为磁性功能材料,制备姜黄素/PLGA磁性微球,考察了乳化方法、高速均质速度、水油相体积比、PLGA用量、水相聚乙烯醇(PVA)质量分数、Fe 3 O 4@OA用量和姜黄素用量对磁性微球粒径的影响,并通过光学显微镜、激光粒度仪、紫外分光光度计、振动样品磁强计、热重分析仪等对其形貌及理化性能进行分析。研究结果显示:姜黄素/PLGA磁性微球的最佳制备工艺为选用搅拌-均质两步乳化法、高速均质15000 r/min、水油相体积比10∶1、PLGA用量100 mg、水相PVA质量分数1%、Fe 3 O 4@OA用量40 mg和姜黄素用量40 mg,此条件下制备的微球表面光滑,粒径较均一,多分散性指数(PDI)为0.38,平均粒径为3.60μm,微球的磁感应强度为14.12(A·m^(2))/kg,含磁量约为27.98%。载药微球的包封率为97.09%,载药率为6.40%,在体外释放实验中表现出明显的缓释效果。
基金supported by the National Natural Science Foundation of China (41602080)Nuclear Energy Development Project from the National Defense Science and Industry Bureau
文摘This paper presents a systematic study of major and trace elements and Sm-Nd isotopes in leucogranites closely related to uranium mineralization in the Gaudeanmus area, Namibia. The results illustrate that the uraniferous leucogranites possess high SiO2 （68.8wt%-76.0wt%, average 73.1wt%） and K （4.05wt%-7.78wt%, average 5.94wt%） contents, and are sub-alkaline and metaluminous to weakly peraluminous, as reflected by A/CNK values of 0.96-1.07 with an average of 1.01. The leucogranites are rich in light rare earth elements （LREE/HREE = 2.53-7.71; （La/Yb）s = 2.14-10.40）, have moderate Eu depletion and high Rb/Sr ratios （2.03-5.50 with an average of 4.36）; meanwhile, they are enriched in Rb, K, Th, U and Pb, and depleted in Ba, Nb, Ta, and Sr. The tNd（t） values of uraninites range from -14.8 to -16.5, and the two-stage Nd model ages are 2.43-2.56 Ga. Detailed elemental and Sm-Nd isotopic geochemical characteristics suggest that the leucogranites were formed in a post- orogenic extensional environment. The U-rich pre-Damara basement was the main source of uranium during the primary mineralization event, which is disseminated in leucogranites, whereas the uranium mineralization in veins possibly resulted from remobilization of the primary uranium minerals.
文摘目的:研究分析可溶性内皮细胞蛋白C受体(Endothelial cell protein C receptor,sEPCR)与膜型EPCR(mEPCR)在参与类风湿关节炎(Rheumatoid Arthritis,RA)疾病进展中的作用。方法:在DBA1/J小鼠尾部注射II型胶原蛋白诱导关节炎小鼠(CIA小鼠),并在两次胶原免疫期间给予实验组小鼠腹腔内注射sEPCR重组蛋白或EPCR腺病毒小干扰载体进行发病前干预;二次加强免疫后的第7 d,对CIA小鼠的关节红肿程度进行连续评分;第28 d处死小鼠,进一步行关节的组织病理学、放射学评估,评价sEPCR和mEPCR对RA疾病进展的作用。结果:(1)与对照组相比,给予sEPCR干预的CIA小鼠关节评分显著降低,组织学和放射学结果显示骨侵蚀程度也较轻;(2)与sEPCR重组蛋白干预相反,mEPCR组CIA小鼠由于EPCR表达下调,CIA小鼠表现为更严重的关节破坏和炎症。结论:EPCR的表达异常与RA的疾病进展密切相关;给予sEPCR治疗可延缓骨破坏,缓解RA的疾病进展;而mEPCR的表达水平降低会介导RA疾病的发生和发展。
