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DC-SIGN与mCEACAM1a分子相互作用调控鼠冠状病毒复制 被引量:3
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作者 张浩旸 郭佳慧 +5 位作者 赵兰娟 姚茜茜 文荣 徐娅佳 王玉燕 叶荣 《微生物与感染》 2018年第3期136-145,共10页
树突细胞特异性细胞间黏附分子-3结合非整合素分子(dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin,DC-SIGN)和肝/淋巴结特异性细胞间黏附分子-3结合非整合素分子(liver/lymph node-specific interc... 树突细胞特异性细胞间黏附分子-3结合非整合素分子(dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin,DC-SIGN)和肝/淋巴结特异性细胞间黏附分子-3结合非整合素分子(liver/lymph node-specific intercellular adhesion molecules-3-grabbing non-integrin,L-SIGN)是钙离子依赖的C型凝集素受体,通过识别病毒粒子表面含甘露聚糖或果糖寡聚糖的分子介导病毒进入细胞,但其在调节病毒复制中的作用较少被关注。本研究通过建立稳定表达DC-SIGN和L-SIGN及其功能域嵌合体的细胞系,分析两者过表达对鼠冠状病毒复制的影响。结果显示,L-SIGN比DC-SIGN更能显著抑制病毒复制,这种差异与两者胞内区序列和基序组成不同有关;鼠冠状病毒感染导致细胞外信号调节激酶(extracellular signal-regulated kinase,ERK)信号通路分子磷酸化下调,过表达DC-SIGN和L-SIGN可抑制这种下调趋势。在没有鼠癌胚抗原相关细胞黏附分子1(mouse carcinoembryonic antigen-related cell adhesion molecule 1,mCEACAM1)存在时,DC-SIGN不能介导病毒感染。这些结果提示,DC-SIGN通过与mCEACAM1a分子相互作用和调节细胞信号通路分子功能以调控鼠冠状病毒复制。 展开更多
关键词 DC-SIGN mCEACAM1a 相互作用 鼠冠状病毒 复制
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Small interfering RNA-mediated inhibition of hepatitis G virus gene expression in human hepatoma cell Huh-7 被引量:1
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作者 CAO Mingmei REN Hao +3 位作者 zhao Ping PAN Wei zhao lanjuan QI Zhongtian 《Science China(Life Sciences)》 SCIE CAS 2005年第1期61-69,共9页
The RNA interference(RNAi)phenomenon is a recently observed process in which the introduction of a double-stranded,small interfering RNA(siRNA)into a cell causes the specific degradation of a homologous single-strande... The RNA interference(RNAi)phenomenon is a recently observed process in which the introduction of a double-stranded,small interfering RNA(siRNA)into a cell causes the specific degradation of a homologous single-stranded RNA.It represents an exciting new technology that could have therapeutic applications for the treatment of viral infections.Since hepatitis G virus(HGV)genome is a positive-sense single-stranded RNA,the replication of HGV does not lead to an integrated DNA genome,suggesting a particularly attractive target for RNAi study that could eliminate viral RNA from infected cells.The eukaryotic expression vector pVAX.EH containing the cDNA sequences of the entire HGV structural genes and hygromycin resistance gene downstream from the encephalomyocarditis virus(ECMV)internal ribosome entry site(IRES)was constructed and transfected into human hepatoma cell Huh-7.The modified cleavage products of the structural proteins of HGV expressed in hygromycin-resistant cell line Huh-7-EH were confirmed by RT-PCR and Western blot methods.Two specific HGV E2 siRNAs(1-E2siRNA,2-E2 siRNA)synthesized with T7 RNA polymerase by transcription in vitro were transfected into the Huh-7-EH cells.With the analyses of Western blot and the formation of hygromycin-resistant colonies,the inhibitions of expression of HGV structural protein by two HGV E2siRNAs were detected and found lasting at least one week.The inhibition of 2-E2 siRNA was stronger and only 1%of the cells treated with 2-E2 siRNA formed hygromycin-resistant colonies.These results support that specific HGV 2-E2 siRNAs mediate the degradation of mRNA spanning from HGV structural gene cDNA to hygromycin resistance gene in a majority of cells.In conclusion,the Huh-7-EH cells expressing HGV structural proteins stably can be used as a cell model for studying the replication of HGV and RNAi and the enlargement of RNAi may exist,in mammalian cells. 展开更多
关键词 RNA interference small interfering RNA hepatitis G virus structural gene cell model.
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基孔肯雅病毒感染性克隆的构建及鉴定 被引量:2
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作者 罗正汉 徐铮昊 +5 位作者 唐海琳 何燕华 彭浩然 赵兰娟 戚中田 赵平 《国际病毒学杂志》 2019年第5期337-340,共4页
目的构建基孔肯雅病毒(chikungunya virus,CHIKV)东中南非型Ross株及该型印度洋分支LR2006株的感染性克隆.方法分别将CHIKV Ross株和LR2006株基因组分段合成,构建完整基因组的转录质粒,转录制备病毒基因组RNA,转染Vero细胞,收集培养上清... 目的构建基孔肯雅病毒(chikungunya virus,CHIKV)东中南非型Ross株及该型印度洋分支LR2006株的感染性克隆.方法分别将CHIKV Ross株和LR2006株基因组分段合成,构建完整基因组的转录质粒,转录制备病毒基因组RNA,转染Vero细胞,收集培养上清,接种Vero细胞扩增病毒,用扩增的病毒感染Huh7细胞,检测病毒蛋白的表达,经滴鼻途径将106 PFU病毒接种给C57BL/6小鼠,观察小鼠的体重改变以及存活情况.结果成功构建了2株CHIKV的全长基因组转录质粒,在RNA转染Vero细胞的上清中检测到感染性CHIKV.Ross株感染的5只小鼠在6天后体重下降(P<0.05),第11天小鼠全部死亡.LR2006株感染的5只小鼠仅1只死亡,且全部小鼠体重均无明显减轻.结论成功构建CHIKV的Ross株和LR2006株感染性克隆,Ross株对C57BL/6小鼠致病性强于LR2006株. 展开更多
关键词 基孔肯雅病毒 Ross株 LR2006株 感染性克隆
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Bivariate genome-wide association study suggests that the DARC gene influences lean body mass and age at menarche
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作者 HAI Rong ZHANG Lei +7 位作者 PEI YuFang zhao lanjuan RAN Shu HAN YingYing ZHU XueZhen SHEN Hui TIAN Qing DENG HongWen 《Science China(Life Sciences)》 SCIE CAS 2012年第6期516-520,共5页
Lean body mass (LBM) and age at menarche (AAM) are two important complex traits for human health. The aim of this study was to identify pleiotropic genes for both traits using a powerful bivariate genome-wide asso... Lean body mass (LBM) and age at menarche (AAM) are two important complex traits for human health. The aim of this study was to identify pleiotropic genes for both traits using a powerful bivariate genome-wide association study (GWAS). Two stud- ies, a discovery study and a replication study, were performed. In the discovery study, 909622 single nucleotide polymor- phisms (SNPs) were genotyped in 801 unrelated female Han Chinese subjects using the Affymetrix human genome-wide SNP array 6.0 platform. Then, a bivariate GWAS was performed to identify the SNPs that may be important for LBM and AAM. In the replication study, significant findings from the discovery study were validated in 1692 unrelated Caucasian female subjects One SNP rs3027009 that was bivafiately associated with left arm lean mass and AAM in the discovery samples (P=7.26x10-6) and in the replication samples (P=0.005) was identified. The SNP is located at the upstream of DARC (Duffy antigen receptor for chemokines) gene, suggesting that DARC may play an important role in regulating the metabolisms of both LBM and AAM. 展开更多
关键词 bivariate genome-wide association study age at menarche lean body mass DARC gene
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