Storm surges are cataclysmic natural disasters that occur along the coasts and are usually accompanied by large waves.The effects of coupled storm surges and waves can pose a significant threat to coastal security.Pre...Storm surges are cataclysmic natural disasters that occur along the coasts and are usually accompanied by large waves.The effects of coupled storm surges and waves can pose a significant threat to coastal security.Previous labo-ratory studies on the effects of storm surges and waves on coastal structures have typically utilized steady water levels and constant wave elements.An indoor simulation of the coupled processes of tides and waves is developed by adding a tide generation system to an existing laboratory wave basin to model continuous dynamic tide levels so that tide generation and wave-making occur synchronously in the pool.Specific experimental methods are given,which are applied to further study waves overtopping on artificial sea dikes and coastal flooding evolution under the coupled actions of tides and waves.The results of the overtopping discharge obtained by the test with a dynamic water level are compared with those obtained from steady water level tests and the existing empirical formula.In addition,the impacts of ecological coastal shelterbelts and structures on coastal flood processes and distributions are also investi-gated.The proposed simulation methods provide a new approach for studying the effects of storm surges and waves on coastal areas.The study also aims to provide a reference for coastal protective engineering.展开更多
Objective To investigate whether Lingbao Huxin Pill(LBHX)protects against acute myocardial infarction(AMI)at the infarct border zone(IBZ)of myocardial tissue by regulating apoptosis and inflammation through the sirtui...Objective To investigate whether Lingbao Huxin Pill(LBHX)protects against acute myocardial infarction(AMI)at the infarct border zone(IBZ)of myocardial tissue by regulating apoptosis and inflammation through the sirtuin 1(SIRT1)-mediated forkhead box protein O1(FOXO1)and nuclear factor-κB(NF-κB)signaling pathways.Methods Six-week-old Wistar rats with normal diet were randomized into the sham,the model,Betaloc(0.9 mg/kg daily),LBHX-L(0.45 mg/kg daily),LBHX-M(0.9 mg/kg daily),LBHX-H(1.8 mg/kg daily),and LBHX+EX527(0.9 mg/kg daily)groups according to the method of random number table,13 in each group.In this study,left anterior descending coronary artery(LADCA)ligation was performed to induce an AMI model in rats.The myocardial infarction area was examined using a 2,3,5-triphenyltetrazolium chloride solution staining assay.A TdT-mediated dUTP nick-end labeling(TUNEL)assay was conducted to assess cardiomyocyte apoptosis in the IBZ.The histopathology of myocardial tissue at the IBZ was assessed with Heidenhain,Masson and hematoxylineosin(HE)staining assays.The expression levels of tumor necrosis factorα(TNF-α),interleukin(IL)-6,IL-1β,and intercellular adhesion molecule-1 were measured using enzyme-linked immunosorbent assays(ELISAs).The mRNA expressions of SIRT1 and FOXO1 were detected by real-time qPCR(RT-qPCR).The protein expressions of SIRT1,FOXO1,SOD2,BAX and NF-κB p65 were detected by Western blot analysis.Results The ligation of the LADCA successfully induced an AMI model.The LBHX pretreatment reduced the infarct size in the AMI rats(P<0.01).The TUNEL assay revealed that LBHX inhibited cardiomyocyte apoptosis at the IBZ.Further,the histological examination showed that the LBHX pretreatment decreased the ischemic area of myocardial tissue(P<0.05),myocardial interstitial collagen deposition(P<0.05)and inflammation at the IBZ.The ELISA results indicated that LBHX decreased the serum levels of inflammatory cytokines in the AMI rats(P<0.05 or P<0.01).Furthermore,Western blot analysis revealed that the LBHX pretreatment upregulated the protein levels of SIRT1,FOXO1 and SOD2(P<0.05)and downregulated NF-κB p65 and BAX expressions(P<0.05).The RT-qPCR results showed that LBHX increased the SIRT1 mRNA and FOXO1 mRNA levels(P<0.05).These protective effects,including inhibiting apoptosis and alleviating inflammation in the IBZ,were partially abolished by EX527,an inhibitor of SIRT1.Conclusion LBHX could protect against AMI by suppressing apoptosis and inflammation in AMI rats and the SIRT1-mediated FOXO1 and NF-κB signaling pathways were involved in the cardioprotection effect of LBHX.展开更多
基金Project(2022YFC2903901) supported by the National Key R&D Project of ChinaProjects(52104111, 52274249) supported by the National Natural Science Foundation of China+3 种基金Project(2021JJ30819) supported by the Natural Science Foundation of Hunan Province,ChinaProject(kfkt2023-01) supported by the Open Fund of State Key Laboratory of Safety Technology of Metal Mines,ChinaProject(2023TJ-X80) supported by the Xiaohe Sci-Tech Talents Special Funding under Hunan Provincial Sci-Tech Talents Sponsorship Program,ChinaProject(2023ZZTS0516) supported by the Fundamental Research Funds for the Central Universities,China。
基金This study was financially supported by the NSFC-Shandong Joint Fund Key Project(Grant No.U1706216)the Innovative Research Foundation of Ship General Performance(Grant No.31422118)the Nanjing Hydraulic Research Institute Special Fund for Basic Scientific Research of Central Public Research Institutes(Grant Nos.Y220013 and Y222004).
文摘Storm surges are cataclysmic natural disasters that occur along the coasts and are usually accompanied by large waves.The effects of coupled storm surges and waves can pose a significant threat to coastal security.Previous labo-ratory studies on the effects of storm surges and waves on coastal structures have typically utilized steady water levels and constant wave elements.An indoor simulation of the coupled processes of tides and waves is developed by adding a tide generation system to an existing laboratory wave basin to model continuous dynamic tide levels so that tide generation and wave-making occur synchronously in the pool.Specific experimental methods are given,which are applied to further study waves overtopping on artificial sea dikes and coastal flooding evolution under the coupled actions of tides and waves.The results of the overtopping discharge obtained by the test with a dynamic water level are compared with those obtained from steady water level tests and the existing empirical formula.In addition,the impacts of ecological coastal shelterbelts and structures on coastal flood processes and distributions are also investi-gated.The proposed simulation methods provide a new approach for studying the effects of storm surges and waves on coastal areas.The study also aims to provide a reference for coastal protective engineering.
基金Supported by the National Natural Science Foundation of China(No.81774141 and No.82074418)。
文摘Objective To investigate whether Lingbao Huxin Pill(LBHX)protects against acute myocardial infarction(AMI)at the infarct border zone(IBZ)of myocardial tissue by regulating apoptosis and inflammation through the sirtuin 1(SIRT1)-mediated forkhead box protein O1(FOXO1)and nuclear factor-κB(NF-κB)signaling pathways.Methods Six-week-old Wistar rats with normal diet were randomized into the sham,the model,Betaloc(0.9 mg/kg daily),LBHX-L(0.45 mg/kg daily),LBHX-M(0.9 mg/kg daily),LBHX-H(1.8 mg/kg daily),and LBHX+EX527(0.9 mg/kg daily)groups according to the method of random number table,13 in each group.In this study,left anterior descending coronary artery(LADCA)ligation was performed to induce an AMI model in rats.The myocardial infarction area was examined using a 2,3,5-triphenyltetrazolium chloride solution staining assay.A TdT-mediated dUTP nick-end labeling(TUNEL)assay was conducted to assess cardiomyocyte apoptosis in the IBZ.The histopathology of myocardial tissue at the IBZ was assessed with Heidenhain,Masson and hematoxylineosin(HE)staining assays.The expression levels of tumor necrosis factorα(TNF-α),interleukin(IL)-6,IL-1β,and intercellular adhesion molecule-1 were measured using enzyme-linked immunosorbent assays(ELISAs).The mRNA expressions of SIRT1 and FOXO1 were detected by real-time qPCR(RT-qPCR).The protein expressions of SIRT1,FOXO1,SOD2,BAX and NF-κB p65 were detected by Western blot analysis.Results The ligation of the LADCA successfully induced an AMI model.The LBHX pretreatment reduced the infarct size in the AMI rats(P<0.01).The TUNEL assay revealed that LBHX inhibited cardiomyocyte apoptosis at the IBZ.Further,the histological examination showed that the LBHX pretreatment decreased the ischemic area of myocardial tissue(P<0.05),myocardial interstitial collagen deposition(P<0.05)and inflammation at the IBZ.The ELISA results indicated that LBHX decreased the serum levels of inflammatory cytokines in the AMI rats(P<0.05 or P<0.01).Furthermore,Western blot analysis revealed that the LBHX pretreatment upregulated the protein levels of SIRT1,FOXO1 and SOD2(P<0.05)and downregulated NF-κB p65 and BAX expressions(P<0.05).The RT-qPCR results showed that LBHX increased the SIRT1 mRNA and FOXO1 mRNA levels(P<0.05).These protective effects,including inhibiting apoptosis and alleviating inflammation in the IBZ,were partially abolished by EX527,an inhibitor of SIRT1.Conclusion LBHX could protect against AMI by suppressing apoptosis and inflammation in AMI rats and the SIRT1-mediated FOXO1 and NF-κB signaling pathways were involved in the cardioprotection effect of LBHX.
