目的从脑胃饥饿素(ghrelin)通路研究应激影响食欲的分子机制。方法32只雄性Wistar大鼠随机分为对照组和应激组,每组16只,每笼2只。采用夹尾应激方法每日2次,连续21 d刺激大鼠,监测各组大鼠的摄食量及体质量;在实验第7、21天每组各选取8...目的从脑胃饥饿素(ghrelin)通路研究应激影响食欲的分子机制。方法32只雄性Wistar大鼠随机分为对照组和应激组,每组16只,每笼2只。采用夹尾应激方法每日2次,连续21 d刺激大鼠,监测各组大鼠的摄食量及体质量;在实验第7、21天每组各选取8只大鼠,E L I S A法检测血清酰基化ghrelin质量浓度;蛋白质印迹法检测大鼠胃及下丘脑中ghrelin蛋白表达,以及下丘脑中腺苷酸活化蛋白激酶α2(Amp-activated protein kinaseα2,AMPKα2)表达水平;实时荧光定量PCR检测胃中ghrelin、生长激素促分泌素受体(growth hormone secretagogue receptor,GHSR)、瘦素(leptin),以及下丘脑中ghrelin、AMPKα2、神经肽Y(neuropeptide Y,NPY)、刺鼠基因相关蛋白(agouti-related protein,AgRP)mRNA水平。结果实验第7天应激组每笼大鼠的总摄食量多于对照组(P<0.05),第21天两组大鼠的总摄食量未见明显差异(P>0.05);在第7、21天,两组大鼠的体质量均无明显差异(P>0.05)。应激可以明显上调血清中酰基化ghrelin水平(P<0.01),胃及下丘脑中ghrelin蛋白(P<0.05)以及下丘脑中AMPKα2蛋白(P<0.01)的表达也均明显上调。应激还能明显上调胃内ghrelin及GHSR mRNA水平(P<0.01),下调胃内leptin mRNA水平(P<0.01);同时上调下丘脑内ghrelin、AMPKα2、NPY及AgRP mRNA水平(P<0.01)。结论夹尾应激能导致大鼠中枢及外周ghrelin水平的上调,可能对大鼠食欲暂时性增加有影响。展开更多
(rTMS) on limb dysfunction in ischemic stroke patients. Methods: A total of 63 stroke patients were divided into an observation group and a control group using the random number table method. Thirty-one patients in th...(rTMS) on limb dysfunction in ischemic stroke patients. Methods: A total of 63 stroke patients were divided into an observation group and a control group using the random number table method. Thirty-one patients in the control group were treated with routine Western medicine combined with rTMS;32 patients in the observation group were treated with EA in addition to the intervention in the control group. The duration of treatment was 3 months. The National Institutes of Health stroke scale (NIHSS), the Fugl-Meyer assessment (FMA), the modified Barthel index (MBI), and the motor evoked potential (MEP) latency of transcranial magnetic stimulation were observed before and after treatment in both groups. Results: Two cases withdrew from the observation group and 1 case withdrew from the control group. After treatment, the NIHSS score in both groups was lower than that before treatment, the FMA and MBI scores were higher than those before treatment, and the latency period of MEP was shorter than that before treatment, and the differences were statistically significant (P<0.05). After treatment, the NIHSS, FMA, and MBI scores and MEP latency period of the observation group improved more than those of the control group, and the differences between the groups were statistically significant (P<0.05). Conclusion: EA combined with rTMS can improve the motor function of limbs in ischemic stroke patients and improve their self-care ability. The mechanism may be related to increasing the excitability of the motor cortex and improving the electrophysiological function of the central nervous system.展开更多
文摘目的从脑胃饥饿素(ghrelin)通路研究应激影响食欲的分子机制。方法32只雄性Wistar大鼠随机分为对照组和应激组,每组16只,每笼2只。采用夹尾应激方法每日2次,连续21 d刺激大鼠,监测各组大鼠的摄食量及体质量;在实验第7、21天每组各选取8只大鼠,E L I S A法检测血清酰基化ghrelin质量浓度;蛋白质印迹法检测大鼠胃及下丘脑中ghrelin蛋白表达,以及下丘脑中腺苷酸活化蛋白激酶α2(Amp-activated protein kinaseα2,AMPKα2)表达水平;实时荧光定量PCR检测胃中ghrelin、生长激素促分泌素受体(growth hormone secretagogue receptor,GHSR)、瘦素(leptin),以及下丘脑中ghrelin、AMPKα2、神经肽Y(neuropeptide Y,NPY)、刺鼠基因相关蛋白(agouti-related protein,AgRP)mRNA水平。结果实验第7天应激组每笼大鼠的总摄食量多于对照组(P<0.05),第21天两组大鼠的总摄食量未见明显差异(P>0.05);在第7、21天,两组大鼠的体质量均无明显差异(P>0.05)。应激可以明显上调血清中酰基化ghrelin水平(P<0.01),胃及下丘脑中ghrelin蛋白(P<0.05)以及下丘脑中AMPKα2蛋白(P<0.01)的表达也均明显上调。应激还能明显上调胃内ghrelin及GHSR mRNA水平(P<0.01),下调胃内leptin mRNA水平(P<0.01);同时上调下丘脑内ghrelin、AMPKα2、NPY及AgRP mRNA水平(P<0.01)。结论夹尾应激能导致大鼠中枢及外周ghrelin水平的上调,可能对大鼠食欲暂时性增加有影响。
文摘(rTMS) on limb dysfunction in ischemic stroke patients. Methods: A total of 63 stroke patients were divided into an observation group and a control group using the random number table method. Thirty-one patients in the control group were treated with routine Western medicine combined with rTMS;32 patients in the observation group were treated with EA in addition to the intervention in the control group. The duration of treatment was 3 months. The National Institutes of Health stroke scale (NIHSS), the Fugl-Meyer assessment (FMA), the modified Barthel index (MBI), and the motor evoked potential (MEP) latency of transcranial magnetic stimulation were observed before and after treatment in both groups. Results: Two cases withdrew from the observation group and 1 case withdrew from the control group. After treatment, the NIHSS score in both groups was lower than that before treatment, the FMA and MBI scores were higher than those before treatment, and the latency period of MEP was shorter than that before treatment, and the differences were statistically significant (P<0.05). After treatment, the NIHSS, FMA, and MBI scores and MEP latency period of the observation group improved more than those of the control group, and the differences between the groups were statistically significant (P<0.05). Conclusion: EA combined with rTMS can improve the motor function of limbs in ischemic stroke patients and improve their self-care ability. The mechanism may be related to increasing the excitability of the motor cortex and improving the electrophysiological function of the central nervous system.
