本研究以55个板栗品种为材料,对这些板栗品种的单粒重量、横径长度和纵径长度等14个表型性状进行测定及分析,部分板栗品种形态性状指标差异不显著,仅利用表型性状很难进行准确的品种鉴定。因此本研究基于板栗全基因组序列开发SSR标记,并...本研究以55个板栗品种为材料,对这些板栗品种的单粒重量、横径长度和纵径长度等14个表型性状进行测定及分析,部分板栗品种形态性状指标差异不显著,仅利用表型性状很难进行准确的品种鉴定。因此本研究基于板栗全基因组序列开发SSR标记,并对55个板栗种质资源进行PCR扩增,利用POPGENE32、PowerMarker V3.25和NTSYS Version 2.10软件对SSR标记数据进行分析,筛选出6对多态性较好的SSR引物。6对引物共检测到31个等位基因,多态位点百分率达100%,多态性信息含量指数、Nei′s基因多样性指数、Shannon′s多态性信息指数、观测杂合度和期望杂合度的平均值分别为0.651、0.696、1.358、0.524、0.703。利用筛选出的6对引物能够明确区分供试的55份板栗品种,并构建了55个板栗品种的字符串、条形码和二维码分子身份证。本研究利用表型性状和SSR标记研究板栗品种的遗传多样性,构建板栗品种的分子身份证,为板栗种质资源的品种鉴定、合理利用和有效保护提供理论参考。展开更多
Fire blight, caused by Erwinia amylovora, is a devastating disease of apples and pears, causing enormous economic losses around the world. The disease is indigenous to North America and has spread to more than 50 coun...Fire blight, caused by Erwinia amylovora, is a devastating disease of apples and pears, causing enormous economic losses around the world. The disease is indigenous to North America and has spread to more than 50 countries since its discovery in 1870 s. Recent reports of the disease in China's neighboring countries, including South Korea, Kyrgyzstan, and Kazakhstan, pose great threat to the world's leading producer of apples and pears. This mini-review intends to provide an update on the disease, pathogen biology, epidemiology, and control. It will also provide some perspectives and suggestions for the apple and pear industry and growers in China, which will face the imminent threat of this devastating disease.展开更多
Bleeding canker,a devastating disease of pear trees(Pyrus pyrifolia L.),was first reported in the 1970 s in Jiangsu,China and more recently in other provinces in China.Trees infected with bleeding canker pathogen,Dick...Bleeding canker,a devastating disease of pear trees(Pyrus pyrifolia L.),was first reported in the 1970 s in Jiangsu,China and more recently in other provinces in China.Trees infected with bleeding canker pathogen,Dickeya fangzhongdai,develop cankers on the trunks and branches,and a rust-colored mixture of bacterial ooze and tree sap could be seen all over the trunks and branches.In this study,we provided detail descriptions of the symptoms and epidemiology of bleeding canker disease.Based on pathogenic and phenotypic characterizations,we identified the causal agent of bleeding canker of pear as D.fangzhongdai.Dickeya fangzhongdai strains isolated from pear were also pathogenic on Solanum tuberosum,Brassica pekinensis,Lycopersicon esculentum,and Phalaenopsis aphrodite based on artificial inoculation,and the pathogen were more virulent on potato than that of D.solani strain.This study provides new information about this disease and bleeding canker disease of pear.展开更多
Dickeya fangzhongdai, the causal agent of bleeding canker of pear, is a new member of the Dickeya genus and the only one that infects woody plants. Recent studies have reclassified several Dickeya isolates as D. fangz...Dickeya fangzhongdai, the causal agent of bleeding canker of pear, is a new member of the Dickeya genus and the only one that infects woody plants. Recent studies have reclassified several Dickeya isolates as D. fangzhongdai, which were isolated from various environments, including water, Phalaenopsis sp. and Aglaonema sp. To provide genomic characterization of D. fangzhongdai isolates from pear, the genomes of D. fangzhongdai strain JS5(=China General Microbiological Culture Collection Center, CGMCC 1.15464 ~T=DSM 101947 ~T), along with two other isolates, LN1 and QZH3, were sequenced and compared to those of other Dickeya spp. Homology greater than 99% was observed among three D. fangzhongdai strains. Plasmid, type IV secretion system(T4 SS) and type IV pili(TFPs) were found in genomes of D. fangzhongdai isolates. Comparative analysis of the type Ⅲ secretion systems(T3 SS), type Ⅲ secretion effectors(T3 SE), plant cell wall degradation enzymes(PCWDE) and membrane transport proteins of Dickeya spp. showed some differences which might reflect the variations of virulence, phylogenetic and phenotypic characteristics of Dickeya spp. In addition, deletion mutant of TFP in D. fangzhongdai JS5 showed no twitching motility and reduced virulence and biofilm formation. The fingdings of the distinctive plasmid, T4 SS and TFPs, as well as the differences of T3 SE, PCWDE and membrane transport proteins make D. fangzhongdai isolates unique. These results also suggested that acquisition of virulence genes by horizontal gene transfer might play some role in the genetic variation of D. fangzhongdai.展开更多
Bleeding canker, caused by Dickeya fangzhongdai, is a devastating disease of pear in China. The bacterium causes cankers, branch die-back, and eventually kills pear trees. The typical sign of bleeding canker infection...Bleeding canker, caused by Dickeya fangzhongdai, is a devastating disease of pear in China. The bacterium causes cankers, branch die-back, and eventually kills pear trees. The typical sign of bleeding canker infection is a rusty-brown bacterial ooze that exudes down from cankers onto branches or trunks. However, early symptoms and signs are inconspicuous, which makes effective disease management difficult. Detection and identification of D. fangzhongdai are time-consuming and difficult because no rapid method exists to date. In this study, a Taq Man real-time PCR assay was developed for D. fangzhongdai based on an elongation factor G(fus A) gene. The real-time PCR assay detected 0.2 pg μL^-1 DNA and 1×10^3 Cfu m L^-1 of D. fangzhongdai. Based on this assay, bleeding canker on asymptomatic pear trees can be diagnosed as early as 5 days after infection. The real-time PCR assay can facilitate disease management by providing early and accurate diagnosis of the bleeding canker disease of pear.展开更多
文摘本研究以55个板栗品种为材料,对这些板栗品种的单粒重量、横径长度和纵径长度等14个表型性状进行测定及分析,部分板栗品种形态性状指标差异不显著,仅利用表型性状很难进行准确的品种鉴定。因此本研究基于板栗全基因组序列开发SSR标记,并对55个板栗种质资源进行PCR扩增,利用POPGENE32、PowerMarker V3.25和NTSYS Version 2.10软件对SSR标记数据进行分析,筛选出6对多态性较好的SSR引物。6对引物共检测到31个等位基因,多态位点百分率达100%,多态性信息含量指数、Nei′s基因多样性指数、Shannon′s多态性信息指数、观测杂合度和期望杂合度的平均值分别为0.651、0.696、1.358、0.524、0.703。利用筛选出的6对引物能够明确区分供试的55份板栗品种,并构建了55个板栗品种的字符串、条形码和二维码分子身份证。本研究利用表型性状和SSR标记研究板栗品种的遗传多样性,构建板栗品种的分子身份证,为板栗种质资源的品种鉴定、合理利用和有效保护提供理论参考。
基金supported by the National Key R&D Program of China(2016YFC1201200)the National Natural Science Foundation of China(31500107 and 31772231)
文摘Fire blight, caused by Erwinia amylovora, is a devastating disease of apples and pears, causing enormous economic losses around the world. The disease is indigenous to North America and has spread to more than 50 countries since its discovery in 1870 s. Recent reports of the disease in China's neighboring countries, including South Korea, Kyrgyzstan, and Kazakhstan, pose great threat to the world's leading producer of apples and pears. This mini-review intends to provide an update on the disease, pathogen biology, epidemiology, and control. It will also provide some perspectives and suggestions for the apple and pear industry and growers in China, which will face the imminent threat of this devastating disease.
基金supported by the 111 International Cooperation Grant 2.0(BP0719029)to Nanjing Agricultural University,China,from the Chinese government and Canadian Interdepartmental funding of Genomics Research and Development Initiatives(GRDI)。
文摘Bleeding canker,a devastating disease of pear trees(Pyrus pyrifolia L.),was first reported in the 1970 s in Jiangsu,China and more recently in other provinces in China.Trees infected with bleeding canker pathogen,Dickeya fangzhongdai,develop cankers on the trunks and branches,and a rust-colored mixture of bacterial ooze and tree sap could be seen all over the trunks and branches.In this study,we provided detail descriptions of the symptoms and epidemiology of bleeding canker disease.Based on pathogenic and phenotypic characterizations,we identified the causal agent of bleeding canker of pear as D.fangzhongdai.Dickeya fangzhongdai strains isolated from pear were also pathogenic on Solanum tuberosum,Brassica pekinensis,Lycopersicon esculentum,and Phalaenopsis aphrodite based on artificial inoculation,and the pathogen were more virulent on potato than that of D.solani strain.This study provides new information about this disease and bleeding canker disease of pear.
基金supported by the 111 International Cooperation Grant 2.0(BP0719029)to Nanjing Agricultural University,China,from the Chinese government and Canadian Interdepartmental funding of Genomics Research and Development Initiatives(GRDI)。
文摘Dickeya fangzhongdai, the causal agent of bleeding canker of pear, is a new member of the Dickeya genus and the only one that infects woody plants. Recent studies have reclassified several Dickeya isolates as D. fangzhongdai, which were isolated from various environments, including water, Phalaenopsis sp. and Aglaonema sp. To provide genomic characterization of D. fangzhongdai isolates from pear, the genomes of D. fangzhongdai strain JS5(=China General Microbiological Culture Collection Center, CGMCC 1.15464 ~T=DSM 101947 ~T), along with two other isolates, LN1 and QZH3, were sequenced and compared to those of other Dickeya spp. Homology greater than 99% was observed among three D. fangzhongdai strains. Plasmid, type IV secretion system(T4 SS) and type IV pili(TFPs) were found in genomes of D. fangzhongdai isolates. Comparative analysis of the type Ⅲ secretion systems(T3 SS), type Ⅲ secretion effectors(T3 SE), plant cell wall degradation enzymes(PCWDE) and membrane transport proteins of Dickeya spp. showed some differences which might reflect the variations of virulence, phylogenetic and phenotypic characteristics of Dickeya spp. In addition, deletion mutant of TFP in D. fangzhongdai JS5 showed no twitching motility and reduced virulence and biofilm formation. The fingdings of the distinctive plasmid, T4 SS and TFPs, as well as the differences of T3 SE, PCWDE and membrane transport proteins make D. fangzhongdai isolates unique. These results also suggested that acquisition of virulence genes by horizontal gene transfer might play some role in the genetic variation of D. fangzhongdai.
基金supported by the National Key Scientific and Technological Projects of China for pear diseases(2018B01005)。
文摘Bleeding canker, caused by Dickeya fangzhongdai, is a devastating disease of pear in China. The bacterium causes cankers, branch die-back, and eventually kills pear trees. The typical sign of bleeding canker infection is a rusty-brown bacterial ooze that exudes down from cankers onto branches or trunks. However, early symptoms and signs are inconspicuous, which makes effective disease management difficult. Detection and identification of D. fangzhongdai are time-consuming and difficult because no rapid method exists to date. In this study, a Taq Man real-time PCR assay was developed for D. fangzhongdai based on an elongation factor G(fus A) gene. The real-time PCR assay detected 0.2 pg μL^-1 DNA and 1×10^3 Cfu m L^-1 of D. fangzhongdai. Based on this assay, bleeding canker on asymptomatic pear trees can be diagnosed as early as 5 days after infection. The real-time PCR assay can facilitate disease management by providing early and accurate diagnosis of the bleeding canker disease of pear.
