The rhizome functions are of great significance to the ecological protection of the western China mining area,whose ecological management can be provided with technical support via accurate calculations of the rhizome...The rhizome functions are of great significance to the ecological protection of the western China mining area,whose ecological management can be provided with technical support via accurate calculations of the rhizome biomass.The rhizome diameter is an important index parameter of rhizome biomass.In this study,we propose an estimation of rhizome diameters based on ground penetrating radar(GPR)-based reverse time migration(RTM)imaging technology.First,the spatial distribution of shallow rhizomes is simulated using the finite difference time domain method.The simulation data are examined via RTM imaging and single-channel rhizome analysis to obtain the rhizome index parameters:Δh,the width of the maximum positive peak amplitude measured at an amplitude of zero,andΔH,the distance between the zero-amplitude position above the largest positive peak in the shallow region and the zero-amplitude position below the largest positive peak in the deeper region.The experiments of physical models verify the effectiveness of the two parameters(Δh andΔH).and indicate that the values ofΔh andΔH are independent of the rhizome burial depth;instead,they are only related to the diameter of the rhizome.For both the numerical simulations and the physical model experiment,the estimation errors ofΔh andΔH for the rhizome diameters can be constrained to less than 6%and 5%,respectively,which shows that the estimation of the rhizome diameters using GPR based RTM imaging technology is reasonable and effective and its high estimation accuracy meets the technical requirements.展开更多
Trichomes are specialized structures developed from epidermal cells and can protect plants against biotic and abiotic stresses.Trichomes cover carrots during the generative phase.However,the morphology of the carrot t...Trichomes are specialized structures developed from epidermal cells and can protect plants against biotic and abiotic stresses.Trichomes cover carrots during the generative phase.However,the morphology of the carrot trichomes and candidate genes controlling the formation of trichomes are still unclear.This study found that carrot trichomes were nonglandular and unbranched hairs distributed on the stem,leaf,petiole,pedicel,and seed of carrot.Resequencing analysis of a trichome mutant with sparse and short trichomes(sst)and a wild type(wt)with long and dense trichomes on carrot stems was conducted.A total of 15396 genes containing nonsynonymous mutations in sst were obtained,including 42 trichomerelated genes.We also analyzed the transcriptome of the trichomes on secondary branches when these secondary branches were 10 cm long between wt and sst and obtained 6576 differentially expressed genes(DEGs),including 24 trichome-related genes.qRT-PCR validation exhibited three significantly up-regulated DEGs,20 significantly downregulated,and one with no difference.We considered both the resequencing and transcriptome sequencing analyses and found that 12 trichome-related genes that were grouped into five transcription factor families containing nonsynonymous mutations and significantly down-regulated in sst.Therefore,these genes are potentially promising candidate genes whose nonsynonymous mutations and down-regulation may result in scarce and short trichomes mutation on carrot stems in sst.展开更多
基金supported by the Open Foundation of the State Key Laboratory of Water Resource Protection and Utilization in Coal Mining(Gant No.SHJT-16-30.18)National Natural Science Foundation of China(No.41602364)+1 种基金National Key R&D Program of China(No.2016YFC0801404)State Key Laboratory of Coal Resources and Safe Mining,China University of Mining and Technology(Beijing)(No.SKLCRSM16DCB14,SKLCRSM17DC01)。
文摘The rhizome functions are of great significance to the ecological protection of the western China mining area,whose ecological management can be provided with technical support via accurate calculations of the rhizome biomass.The rhizome diameter is an important index parameter of rhizome biomass.In this study,we propose an estimation of rhizome diameters based on ground penetrating radar(GPR)-based reverse time migration(RTM)imaging technology.First,the spatial distribution of shallow rhizomes is simulated using the finite difference time domain method.The simulation data are examined via RTM imaging and single-channel rhizome analysis to obtain the rhizome index parameters:Δh,the width of the maximum positive peak amplitude measured at an amplitude of zero,andΔH,the distance between the zero-amplitude position above the largest positive peak in the shallow region and the zero-amplitude position below the largest positive peak in the deeper region.The experiments of physical models verify the effectiveness of the two parameters(Δh andΔH).and indicate that the values ofΔh andΔH are independent of the rhizome burial depth;instead,they are only related to the diameter of the rhizome.For both the numerical simulations and the physical model experiment,the estimation errors ofΔh andΔH for the rhizome diameters can be constrained to less than 6%and 5%,respectively,which shows that the estimation of the rhizome diameters using GPR based RTM imaging technology is reasonable and effective and its high estimation accuracy meets the technical requirements.
基金the Research Project Supported by Shanxi Scholarship Council of China(2021-066)the National Natural Science Foundation of China(31601751)+2 种基金the Key Research and Development Plan of Shanxi Province,China(201903D221063)the Fundamental Research Program of Shanxi Province,China(20210302123412)the Science and Technology Innovation Project of Shanxi Agricultural University,China(2016ZZ02).
文摘Trichomes are specialized structures developed from epidermal cells and can protect plants against biotic and abiotic stresses.Trichomes cover carrots during the generative phase.However,the morphology of the carrot trichomes and candidate genes controlling the formation of trichomes are still unclear.This study found that carrot trichomes were nonglandular and unbranched hairs distributed on the stem,leaf,petiole,pedicel,and seed of carrot.Resequencing analysis of a trichome mutant with sparse and short trichomes(sst)and a wild type(wt)with long and dense trichomes on carrot stems was conducted.A total of 15396 genes containing nonsynonymous mutations in sst were obtained,including 42 trichomerelated genes.We also analyzed the transcriptome of the trichomes on secondary branches when these secondary branches were 10 cm long between wt and sst and obtained 6576 differentially expressed genes(DEGs),including 24 trichome-related genes.qRT-PCR validation exhibited three significantly up-regulated DEGs,20 significantly downregulated,and one with no difference.We considered both the resequencing and transcriptome sequencing analyses and found that 12 trichome-related genes that were grouped into five transcription factor families containing nonsynonymous mutations and significantly down-regulated in sst.Therefore,these genes are potentially promising candidate genes whose nonsynonymous mutations and down-regulation may result in scarce and short trichomes mutation on carrot stems in sst.
