The com borer is a world-wide agricultural pest.In this study,a high-efficiency RNAi method was explored to knock down the neuropeptide F(NPF)to determine if NPF regulates larval feeding in the Asian corn borer,Ostrin...The com borer is a world-wide agricultural pest.In this study,a high-efficiency RNAi method was explored to knock down the neuropeptide F(NPF)to determine if NPF regulates larval feeding in the Asian corn borer,Ostrinia furnacalis.Results showed that the expression peaks of npfmRNA in both midgut and fat body are at 28 h of early 5th instar larvae.When NPF was kno eked dow n either by feedi ng dsNPF-c ontaining artificial diet or direct dsNPF in jection into the 5th in star larvae,npf expression was effectively inhibited in the midgut.Larval feeding,body weight and development time were significantly impacted.In con trast,the expressi on of the npf receptors npfrl and npfr2 in fat body of 5th in star larvae was sign ifica ntly increased by dsNPF injection.These results indicate that NPF can be effectively knoeked down in 0.furnacalis,and dsNPF by injection is an effective and fast way to silence npf expression.This study provides a critical basis for further exploration in mechanism of feeding regulation in 0.furnacalis.展开更多
In sect n europeptides are a group of brain n euro-regulatory factors,which plays very important roles in growth and developme nt,molting and metamorphosis,as well as mating and reproduction.The neuropeptide F(NPF),a ...In sect n europeptides are a group of brain n euro-regulatory factors,which plays very important roles in growth and developme nt,molting and metamorphosis,as well as mating and reproduction.The neuropeptide F(NPF),a multi-functional neuropeptide,is one of neuropeptides identified in numerous insect species,which plays important roles in feeding,metabolism,courtship,reproduction,aggression,ethanol sensitivity,locomotor circadian rhythms,learning and stress responses.These roles of NPF are implemented through NPF receptors(NPFR).The NPFR1,a G protein-coupled receptor with 7 transmembrane domai ns,is one of these receptors and is found to be imports nt for NPF regulation.The NPF usually is con sisted of around 36-40 amino acid residues,but the short neuropeptide F(sNPF)consisted of 7-16 amino acid residues have also been found in some insects.In this review,the structure and function of both NPF and sNPF in in sects are discussed.展开更多
基金This work is supported by the National Natural Science Foundation of China(31730076).
文摘The com borer is a world-wide agricultural pest.In this study,a high-efficiency RNAi method was explored to knock down the neuropeptide F(NPF)to determine if NPF regulates larval feeding in the Asian corn borer,Ostrinia furnacalis.Results showed that the expression peaks of npfmRNA in both midgut and fat body are at 28 h of early 5th instar larvae.When NPF was kno eked dow n either by feedi ng dsNPF-c ontaining artificial diet or direct dsNPF in jection into the 5th in star larvae,npf expression was effectively inhibited in the midgut.Larval feeding,body weight and development time were significantly impacted.In con trast,the expressi on of the npf receptors npfrl and npfr2 in fat body of 5th in star larvae was sign ifica ntly increased by dsNPF injection.These results indicate that NPF can be effectively knoeked down in 0.furnacalis,and dsNPF by injection is an effective and fast way to silence npf expression.This study provides a critical basis for further exploration in mechanism of feeding regulation in 0.furnacalis.
基金This work is supported by grant from the National Natural Science Foundation of China(31730076).
文摘In sect n europeptides are a group of brain n euro-regulatory factors,which plays very important roles in growth and developme nt,molting and metamorphosis,as well as mating and reproduction.The neuropeptide F(NPF),a multi-functional neuropeptide,is one of neuropeptides identified in numerous insect species,which plays important roles in feeding,metabolism,courtship,reproduction,aggression,ethanol sensitivity,locomotor circadian rhythms,learning and stress responses.These roles of NPF are implemented through NPF receptors(NPFR).The NPFR1,a G protein-coupled receptor with 7 transmembrane domai ns,is one of these receptors and is found to be imports nt for NPF regulation.The NPF usually is con sisted of around 36-40 amino acid residues,but the short neuropeptide F(sNPF)consisted of 7-16 amino acid residues have also been found in some insects.In this review,the structure and function of both NPF and sNPF in in sects are discussed.
