目的:运用meta分析方法系统评价中医药治疗男性免疫性不育的有效性和安全性。方法:检索万方数据库、中国生物医学文献数据库、Cochrane Library、维普数据库、PubMed数据库、CNKI(China National Knowledge Infrastructure)数据库,搜集...目的:运用meta分析方法系统评价中医药治疗男性免疫性不育的有效性和安全性。方法:检索万方数据库、中国生物医学文献数据库、Cochrane Library、维普数据库、PubMed数据库、CNKI(China National Knowledge Infrastructure)数据库,搜集中医药治疗男性免疫性不育的随机对照试验(RCT),并进行方法学质量评价,采用RevMan5.4软件进行统计分析与发表偏倚评估。结果:有25篇RCT文献纳入本项研究,总共纳入病例2563例,其中试验组1407例,对照组1155例。meta分析显示中医药治疗男性免疫性不育总有效率(OR=6.35,95%CI:4.96~8.13,P<0.00001)、精浆抗精子抗体转阴率(OR=4.52,95%CI:2.72~7.51,P<0.00001)、血清抗精子抗体转阴率(OR=2.98,95%CI:2.23~3.96,P<0.00001)、精液浓度(MD=15.56,95%CI:11.32~19.79,P<0.00001)、a级精子百分率(MD=3.85,95%CI:1.91~5.79,P=0.00001)、a+b级精子百分率(MD=13.77,95%CI:7.06~20.48,P<0.0001)、精子存活率(MD=10.32,95%CI:6.78~13.86,P<0.00001)、妊娠率(OR=3.53,95%CI:2.68~4.63,P<0.00001)、不良反应(OR=0.06,95%CI:0.01~0.23,P<0.00001)均显著优于单纯西药治疗。中医药治疗男性免疫性不育的精子畸形率与单纯使用西药组疗效差异不明显(MD=-7.53,95%CI:-15.50~0.44,P=0.06)。结论:中医药治疗男性免疫性不育疗效确切、安全性高,是治疗男性免疫性不育的有效方法。展开更多
目的检测NLRP3炎性小体及其下游炎性因子IL-1β/IL-18在类风湿性关节炎(rheumatoid arthritis,RA)患者滑膜被覆细胞及滑膜间质中的表达及分布情况,探讨NLRP3炎症复合体在RA免疫调节中的作用机制。方法采用免疫组化EnVision法检测30例RA...目的检测NLRP3炎性小体及其下游炎性因子IL-1β/IL-18在类风湿性关节炎(rheumatoid arthritis,RA)患者滑膜被覆细胞及滑膜间质中的表达及分布情况,探讨NLRP3炎症复合体在RA免疫调节中的作用机制。方法采用免疫组化EnVision法检测30例RA、10例骨性关节炎(osteoarthritis,OA)患者滑膜被覆细胞及滑膜间质中NLRP3、Caspase-1及其下游炎性因子IL-1β及IL-18的表达。采用Spearman秩相关分析RA患者滑膜组织中NLRP3炎性小体及其下游炎性因子IL-1β/IL-18的表达水平与RA临床和实验室参数的相关性。结果 NLRP3在RA、OA组中的阳性率分别为100.00%和70.00%,Caspase-1在RA、OA组中的阳性率分别为100.00%和50.00%,IL-1β在RA、OA组中的阳性率分别为100.00%和60.00%,IL-18在RA、OA组中的阳性率分别为86.67%和0,NLRP3、Caspase-1、IL-1β及IL-18在RA和OA组间差异均有统计学意义( P <0.05)。IL-1β与RF表达呈显著正相关( P <0.05);NLRP3与CRP、RF、Caspase-1及IL-1β表达均呈显著正相关( P <0.05),而与IL-18表达无相关性( P >0.05)。结论 NLRP3/Caspase-1信号通路因子在RA的发病过程中起重要作用,且与疾病的活动性密切相关。IL-1β的分泌主要是由Caspase-1介导产生,提示抑制NLRP3或Caspase-1可有效下调IL-1β的表达,可能是RA潜在的治疗靶点,具有重要的临床指导意义。展开更多
In order to study the functional and structural alterations of the retina in SD rat model after methanol intoxication,35 rats were divided randomly into five groups administrated with saline,3-day high dose,7-day high...In order to study the functional and structural alterations of the retina in SD rat model after methanol intoxication,35 rats were divided randomly into five groups administrated with saline,3-day high dose,7-day high dose,3-day low dose and 7-day low dose methanol separately.The retinal function of each group was assessed by flash electroretinogram(F-ERG) 3 and 7 days after methanol poisoning.The microstructure and ultrastructure of the retina were observed at the same time.The high-dose methanol intoxication induced irreversible retinal functional and structural damages 3 days after poisoning,which included prolonged latency and reduced amplitude of the Max-reaction of F-ERG.These injuries were aggravated 7 days after poisoning.Meanwhile,the latency and amplitude of the Cone-reaction of F-ERG were also affected 3 days after poisoning,but there were no further worsening tendency 7 days after poisoning.The retinal histological analysis showed cellular edema,heteromorphy and disarrangement,tissular loosen of the inner nuclear layer and photoreceptors layer.The mitochondrial damage began at the photoreceptors layer and developed further into the inner nuclear layer.The low-dose methanol intoxication only caused transient damage of the retina.Our results showed that the function and structure of the photoreceptor and inner nuclear layer were the primary target of methanol intoxication and that the rod cells were more sensitive to methanol intoxication than the cone cells.The mitochondrial damage developed from outer layer to inner layer of the retina.展开更多
文摘目的:运用meta分析方法系统评价中医药治疗男性免疫性不育的有效性和安全性。方法:检索万方数据库、中国生物医学文献数据库、Cochrane Library、维普数据库、PubMed数据库、CNKI(China National Knowledge Infrastructure)数据库,搜集中医药治疗男性免疫性不育的随机对照试验(RCT),并进行方法学质量评价,采用RevMan5.4软件进行统计分析与发表偏倚评估。结果:有25篇RCT文献纳入本项研究,总共纳入病例2563例,其中试验组1407例,对照组1155例。meta分析显示中医药治疗男性免疫性不育总有效率(OR=6.35,95%CI:4.96~8.13,P<0.00001)、精浆抗精子抗体转阴率(OR=4.52,95%CI:2.72~7.51,P<0.00001)、血清抗精子抗体转阴率(OR=2.98,95%CI:2.23~3.96,P<0.00001)、精液浓度(MD=15.56,95%CI:11.32~19.79,P<0.00001)、a级精子百分率(MD=3.85,95%CI:1.91~5.79,P=0.00001)、a+b级精子百分率(MD=13.77,95%CI:7.06~20.48,P<0.0001)、精子存活率(MD=10.32,95%CI:6.78~13.86,P<0.00001)、妊娠率(OR=3.53,95%CI:2.68~4.63,P<0.00001)、不良反应(OR=0.06,95%CI:0.01~0.23,P<0.00001)均显著优于单纯西药治疗。中医药治疗男性免疫性不育的精子畸形率与单纯使用西药组疗效差异不明显(MD=-7.53,95%CI:-15.50~0.44,P=0.06)。结论:中医药治疗男性免疫性不育疗效确切、安全性高,是治疗男性免疫性不育的有效方法。
文摘目的检测NLRP3炎性小体及其下游炎性因子IL-1β/IL-18在类风湿性关节炎(rheumatoid arthritis,RA)患者滑膜被覆细胞及滑膜间质中的表达及分布情况,探讨NLRP3炎症复合体在RA免疫调节中的作用机制。方法采用免疫组化EnVision法检测30例RA、10例骨性关节炎(osteoarthritis,OA)患者滑膜被覆细胞及滑膜间质中NLRP3、Caspase-1及其下游炎性因子IL-1β及IL-18的表达。采用Spearman秩相关分析RA患者滑膜组织中NLRP3炎性小体及其下游炎性因子IL-1β/IL-18的表达水平与RA临床和实验室参数的相关性。结果 NLRP3在RA、OA组中的阳性率分别为100.00%和70.00%,Caspase-1在RA、OA组中的阳性率分别为100.00%和50.00%,IL-1β在RA、OA组中的阳性率分别为100.00%和60.00%,IL-18在RA、OA组中的阳性率分别为86.67%和0,NLRP3、Caspase-1、IL-1β及IL-18在RA和OA组间差异均有统计学意义( P <0.05)。IL-1β与RF表达呈显著正相关( P <0.05);NLRP3与CRP、RF、Caspase-1及IL-1β表达均呈显著正相关( P <0.05),而与IL-18表达无相关性( P >0.05)。结论 NLRP3/Caspase-1信号通路因子在RA的发病过程中起重要作用,且与疾病的活动性密切相关。IL-1β的分泌主要是由Caspase-1介导产生,提示抑制NLRP3或Caspase-1可有效下调IL-1β的表达,可能是RA潜在的治疗靶点,具有重要的临床指导意义。
文摘In order to study the functional and structural alterations of the retina in SD rat model after methanol intoxication,35 rats were divided randomly into five groups administrated with saline,3-day high dose,7-day high dose,3-day low dose and 7-day low dose methanol separately.The retinal function of each group was assessed by flash electroretinogram(F-ERG) 3 and 7 days after methanol poisoning.The microstructure and ultrastructure of the retina were observed at the same time.The high-dose methanol intoxication induced irreversible retinal functional and structural damages 3 days after poisoning,which included prolonged latency and reduced amplitude of the Max-reaction of F-ERG.These injuries were aggravated 7 days after poisoning.Meanwhile,the latency and amplitude of the Cone-reaction of F-ERG were also affected 3 days after poisoning,but there were no further worsening tendency 7 days after poisoning.The retinal histological analysis showed cellular edema,heteromorphy and disarrangement,tissular loosen of the inner nuclear layer and photoreceptors layer.The mitochondrial damage began at the photoreceptors layer and developed further into the inner nuclear layer.The low-dose methanol intoxication only caused transient damage of the retina.Our results showed that the function and structure of the photoreceptor and inner nuclear layer were the primary target of methanol intoxication and that the rod cells were more sensitive to methanol intoxication than the cone cells.The mitochondrial damage developed from outer layer to inner layer of the retina.
