二氢青蒿素对肺癌H460细胞放疗敏感性及miR-219a-5p、CD164表达的影响

目的:探讨二氢青蒿素对肺癌H460细胞放疗敏感性及微小RNA-219a-5p(miR-219a-5p)、钙黏蛋白164(CD164)表达的影响。方法:CCK-8法检测不同浓度(0.01,0.1,1,10,100μmol·L^(-1))二氢青蒿素处理的肺癌H460细胞的增殖抑制率,将肺癌H460细胞设为4组:空白对照组、单纯放疗组、二氢青蒿素组和二氢青蒿素+放疗组,其中空白对照组不进行处理;单纯放疗组采用X射线照射源进行一次性照射,照射剂量为5 Gy,剂量率为1 Gy·min^(-1);二氢青蒿素组加入23.74μmol·L^(-1)二氢青蒿素的培养基;二氢青蒿素+放疗组加入23.74μmol·L^(-1)二氢青蒿素的培养基并采用照射剂量为5 Gy,剂量率为1 Gy·min^(-1)的X射线照射;CCK-8检测细胞增殖活性,流式细胞术检测细胞凋亡及细胞周期分布情况,实时荧光定量PCR法检测细胞miR-219a-5p、CD164 mRNA表达水平,蛋白免疫印迹法检测细胞CD164蛋白表达水平。结果:随着二氢青蒿素浓度的升高,肺癌H460细胞增殖抑制率逐渐升高(P<0.05);与空白对照组比较,单纯放疗组、二氢青蒿素组、二氢青蒿素+放疗组肺癌H460细胞吸光度(A)值、细胞增殖率、S期比例、CD164 mRNA和蛋白表达水平降低,凋亡率、G0/G1期比例、G2/M期比例、miR-219a-5p表达水平升高(P<0.05);与单纯放疗组和二氢青蒿素组比较,二氢青蒿素+放疗组肺癌H460细胞A值、胞增殖率、S期比例、CD164 mRNA和蛋白表达水平降低,凋亡率、G0/G1期比例、G2/M期比例、miR-219a-5p表达水平升高(P<0.05)。结论:二氢青蒿素能够增强肺癌细胞系H460的放疗敏感性,这可能与其通过促进miR-219a-5p的表达来抑制CD164的活性有关。

人工智能辅助CT检查对COVID-19病灶的检测效能分析

目的:探讨人工智能辅助CT检查在COVID-19病变检测及病情评估中的应用价值。方法:回顾性分析41例COVID-19病例,通过统计学分析比较A组(住院医师)、B组(人工智能)、C组(住院医师结合人工智能)对COVID-19病灶的漏诊率、误诊率、阳性预测值、病灶体积、阅片时间的差异。结果:统计学分析显示B组病灶误诊率高于A、C组(P<0.05),A、B、C三组之间的阅片时间两两比较差异均有统计学意义(P<0.05),余组间数据对比无统计学意义(P>0.05)。结论:人工智能辅助诊断可迅速有效识别COVID-19病灶,准确量化病灶体积,与人工阅片相比具有较高的可信度。人工智能辅助诊断与人工阅片相结合可有效降低人工智能辅助诊断的假阳性率,同时提高诊断效率,有效缓解疫情严重地区医疗资源紧张情况。

Quantitative analysis of coronary sinus venous system of normal adults by multi-slice computed tomography

Objective To quantitatively measure the coronary sinus,mainly branch diameter,sectional area in normal adults by multi-slice computed tomography(MSCT)and analyze the relationship between the above imaging indexes and the gender and age.Methods A total of 95 subjects(40 men,55 women;mean age 53.93±14.08 years)examined by MSCT due to suspected coronary heart disease or routine health check-up between September 2015 and May 2016 were included in this study.Coronary artery disease was excluded by MSCT in this cohort:no coronary artery deformity,variation,and all presented with right dominant type of coronary artery and subjects also had no other heart diseases.Diastolic image(75%)of the original image was reconstructed,and quantitative measurements of coronary sinus,mainly branch diameter,sectional area were performed,and the relationship between the above imaging indexes and the gender and age was analyzed.Results 1.The display rates of coronary sinus(CS),posterior interventricular vein(PIV),left ventricular posterior vein(PVLV),the left marginal vein(LMV)were 100%,100%,98% and 46%,respectively.2.There was no significant difference between gender in the diameter and sectional area of CS,PIV and LMV(P>0.05).3.95 patients were divided into three groups according to their age:Group A(≤45)years,n=32;Group B(45-65)years,n=33;and Group C(>65)years,n=30.Coronary sinus vertical diameter(CSVD),left ventricular posterior veins diameter(PVLVD)and left ventricular posterior veins sectional area(PVLVS)were similar among various age group(P>0.05).There were significant differences among the three groups in coronary sinus in anteroposterior diameter(CSAD),coronary sinus diameter(CSD),coronary sinus sectional area(CSS),posterior interventricular vein diameter(PIVD),posterior interventricular vein sectional area(PIVS),the left marginal vein diameter(LMVD)and the left marginal vein sectional area(LMVS),P<0.05.There were significant differences between group A and group B,C(P<0.05).However,values were similar between group B and C,P>0.05.4.The 95% reference ranges of CSVD,CSS,CSD,CSAD,PIVD,PVLVD,LMVD,PIVS,PVLVS,LMVS were(11.95-13.09)mm,(61.10-76.94)mm^2,(8.54-9.54)mm,(8.38-9.35)mm and(3.41-3.97)mm,(3.10-3.56)mm,(2.02-2.45)mm and(10.41-14.08)mm^2,(7.84-10.39)mm^2,(3.39-5.04)mm^2,respectively.Conclusion MSCT supplies objective quantitative measurement of coronary vein main branch diameter,coronary vein sectional area,these measurements might be valuable for the successful implementation of cardiac intervention or surgery by providing the important image reference values of coronary sinus venous system.