目的探讨叶酸对宫颈癌细胞增殖、迁移和凋亡及细胞自噬的影响。方法以宫颈癌细胞株SiHa作为研究对象,用不同质量浓度梯度叶酸培养SiHa细胞,其中中等剂量10.0μg/mL为对照组,0.1μg/mL和1.0μg/mL为低叶酸组,100.0μg/mL和1000.0μg/mL...目的探讨叶酸对宫颈癌细胞增殖、迁移和凋亡及细胞自噬的影响。方法以宫颈癌细胞株SiHa作为研究对象,用不同质量浓度梯度叶酸培养SiHa细胞,其中中等剂量10.0μg/mL为对照组,0.1μg/mL和1.0μg/mL为低叶酸组,100.0μg/mL和1000.0μg/mL为高叶酸组;用CCK-8法检测细胞的增殖活性,划痕实验检测细胞的迁移能力(划痕宽度占初始划痕宽度的比值:划痕宽度比值),流式细胞仪检测细胞的凋亡率;Western blot检测Beclin1、LC3和p62蛋白的表达水平。结果CCK-8法结果显示,质量浓度100.0μg/mL、1000.0μg/mL叶酸明显抑制SiHa细胞增殖活性(0.79±0.01、0.52±0.09 vs 1.24±0.03),且随着叶酸浓度增加,对宫颈癌SiHa细胞增殖的抑制作用越强。细胞划痕实验结果显示,随着叶酸质量浓度的增加,培养48 h的划痕宽度比值增加,叶酸质量浓度为1000.0μg/mL时划痕宽度比值比原始划痕宽度还宽且随着叶酸质量浓度的增加而增加(1.25±0.03 vs 0.59±0.06),对SiHa细胞迁移的抑制作用更强。细胞凋亡实验结果显示,100.0μg/mL、1000.0μg/mL质量浓度的叶酸显著提高SiHa细胞的凋亡率(12.74%±5.12%、26.62%±4.80%vs 6.80%±2.30%),且随着叶酸质量浓度增加,细胞凋亡率越大;随叶酸质量浓度的增加(≥1.0μg/mL),Beclin1、LC3蛋白表达水平升高,p62蛋白表达水平下降。结论叶酸质量浓度≥100.0μg/mL抑制宫颈癌SiHa细胞的增殖及迁移,促进其凋亡,且具有剂量依赖性,可能与细胞自噬的激活有关。展开更多
We examine the structures,cut-off points of transmittance spectra and electric properties of undoped ZnO,SnO_(2) and CdO films by scanning electron microscopy,x-ray diffraction,spectrophotometer and Hall-effect measur...We examine the structures,cut-off points of transmittance spectra and electric properties of undoped ZnO,SnO_(2) and CdO films by scanning electron microscopy,x-ray diffraction,spectrophotometer and Hall-effect measurements,respectively.The films are deposited by using an rf magnetron sputtering system from powder targets in argon and then annealed in vacuum.The structures and properties of the as-deposited films are compared with those of the annealed one.We try to explain the behaviour of charge carriers based on the semiconductor physics theory.展开更多
文摘目的探讨叶酸对宫颈癌细胞增殖、迁移和凋亡及细胞自噬的影响。方法以宫颈癌细胞株SiHa作为研究对象,用不同质量浓度梯度叶酸培养SiHa细胞,其中中等剂量10.0μg/mL为对照组,0.1μg/mL和1.0μg/mL为低叶酸组,100.0μg/mL和1000.0μg/mL为高叶酸组;用CCK-8法检测细胞的增殖活性,划痕实验检测细胞的迁移能力(划痕宽度占初始划痕宽度的比值:划痕宽度比值),流式细胞仪检测细胞的凋亡率;Western blot检测Beclin1、LC3和p62蛋白的表达水平。结果CCK-8法结果显示,质量浓度100.0μg/mL、1000.0μg/mL叶酸明显抑制SiHa细胞增殖活性(0.79±0.01、0.52±0.09 vs 1.24±0.03),且随着叶酸浓度增加,对宫颈癌SiHa细胞增殖的抑制作用越强。细胞划痕实验结果显示,随着叶酸质量浓度的增加,培养48 h的划痕宽度比值增加,叶酸质量浓度为1000.0μg/mL时划痕宽度比值比原始划痕宽度还宽且随着叶酸质量浓度的增加而增加(1.25±0.03 vs 0.59±0.06),对SiHa细胞迁移的抑制作用更强。细胞凋亡实验结果显示,100.0μg/mL、1000.0μg/mL质量浓度的叶酸显著提高SiHa细胞的凋亡率(12.74%±5.12%、26.62%±4.80%vs 6.80%±2.30%),且随着叶酸质量浓度增加,细胞凋亡率越大;随叶酸质量浓度的增加(≥1.0μg/mL),Beclin1、LC3蛋白表达水平升高,p62蛋白表达水平下降。结论叶酸质量浓度≥100.0μg/mL抑制宫颈癌SiHa细胞的增殖及迁移,促进其凋亡,且具有剂量依赖性,可能与细胞自噬的激活有关。
基金Supported by the National Natural Science Foundation of China under Grant Nos.50872048 and 51172101.
文摘We examine the structures,cut-off points of transmittance spectra and electric properties of undoped ZnO,SnO_(2) and CdO films by scanning electron microscopy,x-ray diffraction,spectrophotometer and Hall-effect measurements,respectively.The films are deposited by using an rf magnetron sputtering system from powder targets in argon and then annealed in vacuum.The structures and properties of the as-deposited films are compared with those of the annealed one.We try to explain the behaviour of charge carriers based on the semiconductor physics theory.
