One SMV resistant soybean line (95-5383) was crossed with four susceptible soybean varieties/ line (HB1, Tiefeng21, Amsoy, Williams) and one resistant introduced line PI486355. Their F, and F2 individuals were identif...One SMV resistant soybean line (95-5383) was crossed with four susceptible soybean varieties/ line (HB1, Tiefeng21, Amsoy, Williams) and one resistant introduced line PI486355. Their F, and F2 individuals were identified for SMV resistance by inoculation with SMV3. The results showed that in the four crosses of resistant X susceptible, F1 were susceptible and the ratio of F2 populations was 1 resistant : 3 susceptible (mosaic and necrosis), indicating that 95-5383 carries one recessive gene that confer resistance to SMV3. There is segregation of susceptibility in F2 progenies from the cross of 95-5383 X PI486355, indicating that the SMV3 resistant gene in 95-5383 is located at different locus from PI486355. By bulked segregating analysis (BSA) in F2 populations of 95-5383 X HB1, one codominant RAPD marker OPN11980/1070 closely linked to SMV3 resistance gene amplified with RAPD primer OPN11 was identified. The DNA fragment OPNll980 was amplified in resistant parent 95-5383 and resistant bulk, and OPN111070 was amplified in susceptible parent HB1 and susceptible bulk. OPNll980/1070 was amplified in F,. Identification of the markers in F2 plants showed that the codominant marker OPNll980/1070 is closely linked to the SMV resistance locus in 95-5383, with genetic distance of 2.1cM.展开更多
文摘One SMV resistant soybean line (95-5383) was crossed with four susceptible soybean varieties/ line (HB1, Tiefeng21, Amsoy, Williams) and one resistant introduced line PI486355. Their F, and F2 individuals were identified for SMV resistance by inoculation with SMV3. The results showed that in the four crosses of resistant X susceptible, F1 were susceptible and the ratio of F2 populations was 1 resistant : 3 susceptible (mosaic and necrosis), indicating that 95-5383 carries one recessive gene that confer resistance to SMV3. There is segregation of susceptibility in F2 progenies from the cross of 95-5383 X PI486355, indicating that the SMV3 resistant gene in 95-5383 is located at different locus from PI486355. By bulked segregating analysis (BSA) in F2 populations of 95-5383 X HB1, one codominant RAPD marker OPN11980/1070 closely linked to SMV3 resistance gene amplified with RAPD primer OPN11 was identified. The DNA fragment OPNll980 was amplified in resistant parent 95-5383 and resistant bulk, and OPN111070 was amplified in susceptible parent HB1 and susceptible bulk. OPNll980/1070 was amplified in F,. Identification of the markers in F2 plants showed that the codominant marker OPNll980/1070 is closely linked to the SMV resistance locus in 95-5383, with genetic distance of 2.1cM.
