Protein and starch are the most important traits in determining processing quality in wheat. In order to understand the genetic basis of the influence of Waxy protein （Wx） and high molecular weight gluten subunit （...Protein and starch are the most important traits in determining processing quality in wheat. In order to understand the genetic basis of the influence of Waxy protein （Wx） and high molecular weight gluten subunit （HMW-GS） on processing quality, 256 recombinant inbred lines （RILs） derived from the cross of waxy wheat Nuomai 1 and Gaocheng 8901 were used as mapping population. DArT （diversity arrays technology）, SSR （simple sequence repeat）, HMW-GS, and Wx markers were used to construct the molecular genetic linkage map. QTLs for mixing peak time （MPT）, mixing peak value （MPV）, mixing peak width （MPW）, and mixing peak integral （MPI） of Mixograph parameters were evaluated in three different environments. The genetic map comprised 498 markers, including 479 DArT, 14 SSR, 2 HMW-GS, and 3 Wx protein markers, covering 4 229.7 cM with an average distance of 9.77 cM. These markers were identified on 21 chromosomes. Eighteen additive QTLs were detected in three different environments, which were distributed on chromosomes 1A, 1B, 1D, 4A, 6A, and 7D. QMPT-1D.1 and QMPT-1D.2 were close to the Glu-D1 marker accounting for 35.2, 22.22 and 36.57% of the phenotypic variance in three environments, respectively. QMPV-1D and QMPV-4A were detected in all environments, and QMPV-4A was the nearest to Wx-B1. One minor QTL, QMPI-1A, was detected under three environments with the genetic distances of 0.9 cM from the nearest marker Glu-A1, explaining from 5.31 to 6.67% of the phenotypic variance. Three pairs of epistatic QTLs were identified on chromosomes 2D and 4A. Therefore, this genetic map is very important and useful for quality trait related QTL mapping in wheat. In addition, the finding of several major QTLs, based on the genetic analyses, further suggested the importance of Glu-1 loci on dough mixing characteristics.展开更多
目的探讨循环滤泡辅助T细胞(circulating follicular helper T Cells,cTfh)在老年T细胞淋巴瘤病人中的变化特点。方法本研究共纳入T细胞淋巴瘤感染病人、健康对照者以及采用药物治疗的T细胞淋巴瘤病人各55例。采用流式细胞仪检测cTfh细...目的探讨循环滤泡辅助T细胞(circulating follicular helper T Cells,cTfh)在老年T细胞淋巴瘤病人中的变化特点。方法本研究共纳入T细胞淋巴瘤感染病人、健康对照者以及采用药物治疗的T细胞淋巴瘤病人各55例。采用流式细胞仪检测cTfh细胞表面相关分子标志物CD134,采用ELISA法检测细胞表面相关分子标志物分泌的IL-4、IL-21等相关细胞因子,比较3组结果差异。结果T细胞淋巴瘤感染组外周血CD134比例为(22.30±6.45)%,高于健康对照组[(3.70±2.18)%]以及药物治疗组[(12.90±4.68)%],差异均有统计学意义(P<0.05)。T细胞淋巴瘤感染组血清中IL-21、IL-4的水平明显高于健康对照组及药物治疗组,且药物治疗组明显高于健康对照组,差异均有统计学意义(P<0.05)。结论外周血cTfh及其分泌的相关细胞因子可能参与了老年T细胞淋巴瘤病人免疫应答发病机制,并在其中起重要作用。展开更多
基金supported by the National Natural Science Foundation of China(31171554)the National Basic Research Program of China(2009CB118301)the Natural Science Foundation of Shandong Province,China(ZR2009DQ009)
文摘Protein and starch are the most important traits in determining processing quality in wheat. In order to understand the genetic basis of the influence of Waxy protein （Wx） and high molecular weight gluten subunit （HMW-GS） on processing quality, 256 recombinant inbred lines （RILs） derived from the cross of waxy wheat Nuomai 1 and Gaocheng 8901 were used as mapping population. DArT （diversity arrays technology）, SSR （simple sequence repeat）, HMW-GS, and Wx markers were used to construct the molecular genetic linkage map. QTLs for mixing peak time （MPT）, mixing peak value （MPV）, mixing peak width （MPW）, and mixing peak integral （MPI） of Mixograph parameters were evaluated in three different environments. The genetic map comprised 498 markers, including 479 DArT, 14 SSR, 2 HMW-GS, and 3 Wx protein markers, covering 4 229.7 cM with an average distance of 9.77 cM. These markers were identified on 21 chromosomes. Eighteen additive QTLs were detected in three different environments, which were distributed on chromosomes 1A, 1B, 1D, 4A, 6A, and 7D. QMPT-1D.1 and QMPT-1D.2 were close to the Glu-D1 marker accounting for 35.2, 22.22 and 36.57% of the phenotypic variance in three environments, respectively. QMPV-1D and QMPV-4A were detected in all environments, and QMPV-4A was the nearest to Wx-B1. One minor QTL, QMPI-1A, was detected under three environments with the genetic distances of 0.9 cM from the nearest marker Glu-A1, explaining from 5.31 to 6.67% of the phenotypic variance. Three pairs of epistatic QTLs were identified on chromosomes 2D and 4A. Therefore, this genetic map is very important and useful for quality trait related QTL mapping in wheat. In addition, the finding of several major QTLs, based on the genetic analyses, further suggested the importance of Glu-1 loci on dough mixing characteristics.
文摘目的探讨循环滤泡辅助T细胞(circulating follicular helper T Cells,cTfh)在老年T细胞淋巴瘤病人中的变化特点。方法本研究共纳入T细胞淋巴瘤感染病人、健康对照者以及采用药物治疗的T细胞淋巴瘤病人各55例。采用流式细胞仪检测cTfh细胞表面相关分子标志物CD134,采用ELISA法检测细胞表面相关分子标志物分泌的IL-4、IL-21等相关细胞因子,比较3组结果差异。结果T细胞淋巴瘤感染组外周血CD134比例为(22.30±6.45)%,高于健康对照组[(3.70±2.18)%]以及药物治疗组[(12.90±4.68)%],差异均有统计学意义(P<0.05)。T细胞淋巴瘤感染组血清中IL-21、IL-4的水平明显高于健康对照组及药物治疗组,且药物治疗组明显高于健康对照组,差异均有统计学意义(P<0.05)。结论外周血cTfh及其分泌的相关细胞因子可能参与了老年T细胞淋巴瘤病人免疫应答发病机制,并在其中起重要作用。
