目的观察毛冬青甲素(ilexonin A, IA)干预大鼠骨髓间充质干细胞(BMSCs)后基质细胞衍生因子(SDF-1)特异性受体CXCR4和Wnt通路中β-连环蛋白(β-catenin)、糖原合成激酶(GSK-3β)的表达情况,探讨IA预处理促进BMSCs体外迁移的分子机制。方...目的观察毛冬青甲素(ilexonin A, IA)干预大鼠骨髓间充质干细胞(BMSCs)后基质细胞衍生因子(SDF-1)特异性受体CXCR4和Wnt通路中β-连环蛋白(β-catenin)、糖原合成激酶(GSK-3β)的表达情况,探讨IA预处理促进BMSCs体外迁移的分子机制。方法采用全骨髓贴壁法体外分离、培养、纯化大鼠并行BMSCs鉴定。将BMSCs分为对照组、Wnt3a组、IA组、IA+Wnt3a组、IA+Dkk1组、Dkk1组。以Wnt信号通路的激动剂Wnt3a、抑制剂Dkk1干预,Western blot法观察β-catenin、GSK-3β与CXCR4的表达关系。结果与对照组比较,Wnt3a组、IA组CXCR4、β-catenin蛋白表达显著增强、GSK-3β蛋白表达显著减弱;(P<0.05),Dkk1组CXCR4、β-catenin蛋白表达显著减弱(P<0.05);与Wnt3a组、IA组分别比较,IA+Wnt3a组CXCR4蛋白显著增强,GSK-3β蛋白表达显著减弱(P<0.05);与Dkk1组比较,IA+Dkk1组CXCR4、β-catenin蛋白表达显著增强,GSK-3β蛋白表达显著减弱(P<0.05);与IA组比较,IA+Dkk1组β-catenin的表达显著减弱,GSK-3β蛋白表达显著增强(P<0.05)。结论 IA上调CXCR4的表达,可能与BMSCs的Wnt/β-catenin信号通路激活有关,发挥其促迁移的作用。展开更多
Objective: To observe the intervention effects of Tiaobu Xinshen Recipe(调补心肾方, TXR) on patients with mild cognitive impairment caused by Alzheimer’s disease(MCI-AD). Methods: Totally 88 MCI-AD patients with synd...Objective: To observe the intervention effects of Tiaobu Xinshen Recipe(调补心肾方, TXR) on patients with mild cognitive impairment caused by Alzheimer’s disease(MCI-AD). Methods: Totally 88 MCI-AD patients with syndrome of Xin(Heart) and Shen(Kidney) deficiency were assigned to the experimental group(47 cases, treated with TXR) and the control group(41 cases, treated with donepezil hydrochloride) using a random number table. Final recruited qualified patients were 44 cases in the experimental group and 39 cases in the control group. The therapeutic course was 12 weeks. Neuropsychological scales [mini mental state examination(MMSE) and Montreal cognitive assessment(MoCA)], and Chinese medicine(CM) dementia syndromes scales were performed in all patients, and results were compared between groups or intra-group before and after treatment. Results: MMSE and Mo CA scores of the two groups were increased after treatment compared with those before treatment(P<0.05). But there was no statistical difference in MMSE or MOCA scores after treatment between the two groups(P>0.05). CM dementia syndrome score was significantly decreased after treatment in the experimental group compared with the control group(P<0.01). Visual spatial and executive function scores and delayed recall scores of the two groups were increased compared with those before treatment(P<0.01). Conclusion: TXR could effectively improve cognitive impairment of MCI-AD patients with syndrome of Xin and Shen deficiency.展开更多
文摘目的观察毛冬青甲素(ilexonin A, IA)干预大鼠骨髓间充质干细胞(BMSCs)后基质细胞衍生因子(SDF-1)特异性受体CXCR4和Wnt通路中β-连环蛋白(β-catenin)、糖原合成激酶(GSK-3β)的表达情况,探讨IA预处理促进BMSCs体外迁移的分子机制。方法采用全骨髓贴壁法体外分离、培养、纯化大鼠并行BMSCs鉴定。将BMSCs分为对照组、Wnt3a组、IA组、IA+Wnt3a组、IA+Dkk1组、Dkk1组。以Wnt信号通路的激动剂Wnt3a、抑制剂Dkk1干预,Western blot法观察β-catenin、GSK-3β与CXCR4的表达关系。结果与对照组比较,Wnt3a组、IA组CXCR4、β-catenin蛋白表达显著增强、GSK-3β蛋白表达显著减弱;(P<0.05),Dkk1组CXCR4、β-catenin蛋白表达显著减弱(P<0.05);与Wnt3a组、IA组分别比较,IA+Wnt3a组CXCR4蛋白显著增强,GSK-3β蛋白表达显著减弱(P<0.05);与Dkk1组比较,IA+Dkk1组CXCR4、β-catenin蛋白表达显著增强,GSK-3β蛋白表达显著减弱(P<0.05);与IA组比较,IA+Dkk1组β-catenin的表达显著减弱,GSK-3β蛋白表达显著增强(P<0.05)。结论 IA上调CXCR4的表达,可能与BMSCs的Wnt/β-catenin信号通路激活有关,发挥其促迁移的作用。
基金Supported by Fujian Provincial Research Project of Traditional Chinese Medicine(No.wzkf201309)
文摘Objective: To observe the intervention effects of Tiaobu Xinshen Recipe(调补心肾方, TXR) on patients with mild cognitive impairment caused by Alzheimer’s disease(MCI-AD). Methods: Totally 88 MCI-AD patients with syndrome of Xin(Heart) and Shen(Kidney) deficiency were assigned to the experimental group(47 cases, treated with TXR) and the control group(41 cases, treated with donepezil hydrochloride) using a random number table. Final recruited qualified patients were 44 cases in the experimental group and 39 cases in the control group. The therapeutic course was 12 weeks. Neuropsychological scales [mini mental state examination(MMSE) and Montreal cognitive assessment(MoCA)], and Chinese medicine(CM) dementia syndromes scales were performed in all patients, and results were compared between groups or intra-group before and after treatment. Results: MMSE and Mo CA scores of the two groups were increased after treatment compared with those before treatment(P<0.05). But there was no statistical difference in MMSE or MOCA scores after treatment between the two groups(P>0.05). CM dementia syndrome score was significantly decreased after treatment in the experimental group compared with the control group(P<0.01). Visual spatial and executive function scores and delayed recall scores of the two groups were increased compared with those before treatment(P<0.01). Conclusion: TXR could effectively improve cognitive impairment of MCI-AD patients with syndrome of Xin and Shen deficiency.
