基于斑马鱼模型对药食同源中药饮品的抗氧化和抗疲劳功效研究

[目的]对2种药食同源中药饮品(香蜂悦颜饮、参杞固元饮)成分进行初步分析,并利用斑马鱼功效模型评价2种饮品的抗氧化和抗疲劳作用。[方法]通过UHPLC-LTQ-Orbitrap-MS液质联用技术,用反相C_(18)色谱柱,采用双压线性离子阱质谱正/负离子双重扫描方式,对样品进行成分分析;对水溶给予甲萘醌方法建立的斑马鱼氧化损伤模型加入梯度浓度(25、50、100μg/mL)的香蜂悦颜饮稀释液干预,同时设置正常组、阳性药组,处理24 h后,Cell ROX避光染色,在荧光显微镜下观察拍照,计算斑马鱼的卵黄囊荧光强度;选择5日龄野生型斑马鱼,加入梯度浓度(25、50、100μg/mL)参杞固元饮稀释液干预,同时设置正常对照组和阳性药对照组,处理18 h后,给药组水溶给予无水亚硫酸钠建立斑马鱼疲劳模型,并利用行为分析仪分析斑马鱼总运动距离。[结果]根据质谱检测数据,香蜂悦颜饮、参杞固元饮分别检测出63和60个化合物。与模型组相比,香蜂悦颜饮干预下的斑马鱼卵黄囊荧光强度显著变弱(P<0.01);与模型对照组相比,参杞固元饮干预下的斑马鱼总运动距离显著更远(P<0.05)。[结论]2种药食同源中药饮品分别具有明确的抗氧化和抗疲劳作用。

姜黄素减肥胶囊中功效成分含量测定方法学验证实验设计

目的设计姜黄素减肥胶囊中功效成分姜黄素含量测定方法学验证综合试验。方法以十八烷基硅烷键合硅胶为填充剂,乙腈-4%冰醋酸(48∶52)为流动相,检测波长430 nm,流速为1 ml/min进行检测,以峰面积外标法定量。结果在选定的色谱条件下,本方法的专属性、线性、精密度、准确度、稳定性满足要求。结论经过方法学验证,本方法简便快捷、准确度高、重复性好,是检测姜黄素减肥胶囊中姜黄素的有效定量方法。

Early outcomes and analysis of factors influencing in-hospital mortality after concomitant heart valve replacement and coronary artery bypass grafting

Background Objective:To investigate the perioperative precautions,surgical methods and early clinical efficacy of patients with valvular heart disease complicated by coronary atherosclerotic heart disease treated with coronary artery bypass grafting at the same time as heart valve replacement and to analyze the relevant factors affecting the death of patients during hospitalization after surgery.Methods:Between January 2020 and September 2021,a total of 103 patients who underwent simultaneous heart valve replacement and coronary artery bypass grafting for heart valve disease in conjunction with coronary atherosclerotic heart disease at the Department of cardiac surgery,Guangdong Cardiovascular Institute were retrospectively evaluated for clinical data,and a systematic review and summary of all preoperative data,surgical methods,intraoperative data,and major complications were performed.The variations in each cardiotocography indicator were examined before and after surgery to determine surgical effectiveness and statistical perioperative patient mortality.After integrating the Sino SCORE risk assessment system and analyzing the case features included in this data,12 alternative risk variables for mortality were identified and submitted to univariate and multivariate analysis using logistic regression.After performing a univariate analysis of the alternative risk factors,the option of P<0.05 was considered a risk factor and included in the multivariate analysis,followed by a multivariate logistic regression analysis in which the factors were determined to be independent risk factors with P<0.05,and their OR values and 95 percent confidence intervals(CIs)were calculated to analyze the associated factors affecting patients’early prognosis systematically.Results:Of the 103 patients,91 were successfully discharged from the hospital,12 died,11.7%of the deaths were due to the following causes:postoperative low cardiac output syndrome,multiple organ failure,and severe infection;Among all the 103 patients,7 patients underwent rebleeding and hemostasis after surgery,6 patients required dialysis after surgery,28 patients underwent IABP supportive care after surgery because of the occurrence of low cardiac output syndrome,1 patient assisted by ECMO after surgery,and patients discharged successfully had significantly less LVEDD and LVESD in the early postoperative period,compared with those before surgery(P=0.000;P=0.000).Age>70 years,preoperative combined hyperlipidemia,and history of the cerebrovascular accident were the three independent risk factors that may have contributed to in-hospital mortality in patients undergoing CABG valve replacement.Conclusions:1.The diastolic function of the majority of patients with valvular heart disease and coronary atherosclerotic heart disease improved significantly in the early stages after coronary artery bypass grafting and heart valve replacement.2.Age>70 years,preoperative combined hyperlipidemia,and a history of the cerebrovascular accident were three independent risk factors for in-hospital death in patients receiving CABG valve replacement.

Impact of right ventricle to pulmonary artery connection and systemic-to-pulmonary artery shunt surgery on promoting the development of pulmonary vasculature in patients with pulmonary atresia with ventricular septal defect

Background Pulmonary atresia with ventricular septal defect(PA/VSD)is a rare and complex congenital heart disease(CHD).The optimal palliative surgical strategy for pulmonary atresia with ventricular septal defect(PA/VSD)in neonates and young infants is controversial.Surgery mainly includes the following two options,right ventricle to pulmonary artery connection(RV-PA)and systemic-to-pulmonary artery shunt surgery(SPS).Objectives:To determine the impact of the right ventricle to pulmonary artery connection or systemic-to-pulmonary artery shunt surgery as the initially palliated surgical strategy on promoting the development of pulmonary vasculature in patients with pulmonary atresia and ventricular septal defect(PA/VSD).Methods:From January 2010 to December 2019,104 patients with PA/VSD in Guangdong Cardiovascular Institute who underwent right ventricle to pulmonary artery connection or systemic-to-pulmonary artery shunt surgery as the initially palliated surgical strategy to promoting the development of pulmonary vasculature were identified and enrolled in this retrospective study.The cohort was divided into two groups:group-Right Ventricle to Pulmonary Artery Connection(RV-PA),who underwent initial palliation with staged repair(n=51),and group-Systemic-to-Pulmonary Artery Shunt(SPS)(n=53).Preoperative and postoperative the development of pulmonary vasculature data have been collected and compared.Results:Before the surgery,the Mc Goon Ratio of the RV-PA group significantly high than and the SPS group(P<0.05).After the surgery,in the RV-PA group,the Nakata index,Mc Goon Ratio score significantly increased during the interstage period(P<0.01).At the meantime,the HCT,RBC and HB significantly reduced in the RV-PA group compared with the SPS group(P<0.05).The shunt group performed better in these areas:length of hospital stay,ACC time and CPB time(P<0.05).Conclusion:Compared with systemic-to-pulmonary artery shunt,right ventricle to pulmonary artery connection is more effective to promote the development of the pulmonary vasculature for pulmonary atresia with ventricular septal defect.