Objective:To explore the curative effect of nano-Kuiyangye on radiation esophagitis in Wistar rats,and to explore its possible mechanism.Methods:Wistar rats were irradiated locally with 30Gy rays to establish an anima...Objective:To explore the curative effect of nano-Kuiyangye on radiation esophagitis in Wistar rats,and to explore its possible mechanism.Methods:Wistar rats were irradiated locally with 30Gy rays to establish an animal model of radiation esophagitis in rats.After irradiation,nano-Kuiyangye,traditional Chinese medicine ulcer solution,Kangfuxin solution,nano-hydrotalcite matrix,and distilled water were used to intervene continuously for 7 d,during which the body weight and food intake of the rats were recorded.On day 7,blood was collected from the abdominal aorta under anesthesia,and the serum was obtained by centrifugation.The expression levels of pain-related factors prostaglandin-2(PGE-2),substance P(SP),and calcitonin gene-related peptide(CGRP)were detected by ELISA.Rats were sacrificed after blood collection,and full-length esophageal tissues were taken.Hematoxylin-eosin(HE)staining was performed to analyze the pathological changes of the rat esophagus,and Western Blotting was used to detect the expression of nuclear factor-κB(NF-κB)inflammatory protein.Results:Compared with the control group,the total food intake and body weight of the rats within 7 d after modeling were significantly decreased(P<0.05),and the expressions of pain-related factors PGE-2,SP,and CGRP were higher than those of the control group(P<0.05).,the esophageal pathological damage score increased(P<0.05),and the expression of NF-κB inflammatory protein increased(P<0.05);after treatment,the total food intake of the rats in the nano-Kuiyangye intervention group within 7 days after modeling was higher than that in other groups(P<0.05),the expressions of pain-related factors PGE-2,SP,and CGRP were lower than those in the model group(P<0.05),the esophageal pathological damage score was lower(P<0.05),and the expression of NF-κB inflammatory proteins was lower(P<0.05).Conclusion:Nano-Kuiyangye increases the food intake of rats with radiation esophagitis,reduces esophageal tissue damage,and reduces the concentration of serum pain factors;the anti-inflammatory effect of nano-Kuiyangye may be related to the reduction of NF-κB inflammatory factor levels.展开更多
目的通过对药物临床试验临床研究协调员(clinical research coordinator,CRC)进行调查,提出建议和优化CRC管理体系,以提高药物临床试验质量。方法于2021年4月6日至4月12日期间通过问卷星向调查对象发放自行设计的问卷进行工作现状调查分...目的通过对药物临床试验临床研究协调员(clinical research coordinator,CRC)进行调查,提出建议和优化CRC管理体系,以提高药物临床试验质量。方法于2021年4月6日至4月12日期间通过问卷星向调查对象发放自行设计的问卷进行工作现状调查分析,回收有效问卷137份。结果CRC来源复杂良莠不齐,大部分CRC目前工作强度过大,对参加的培训满意度较低,且严重缺乏归属感。结论医疗机构应建立有效的SMO和CRC的分级量化管理制度,完善人员准入和劝退机制,加强研究者和CRC有效培训,并搭建合作平台进一步提高CRC的工作质量,保障药物临床试验质量。展开更多
1文献来源Al-Batran SE,Homann N,Pauligk C,et al.Perioperative chemotherapy with fluorouracil plus leucovorin,oxaliplatin,and docetaxel versus fluorouracil or capecitabine plus cisplatin and epirubicin for locally advan...1文献来源Al-Batran SE,Homann N,Pauligk C,et al.Perioperative chemotherapy with fluorouracil plus leucovorin,oxaliplatin,and docetaxel versus fluorouracil or capecitabine plus cisplatin and epirubicin for locally advanced,resectable gastric or gastro-oesophageal junction adenocarcinoma(FLOT4):A randomised,phase 2/3 trial[J].Lancet,2019,393(10184):1948-1957.展开更多
Objective:To evaluate the association between Chinese medicine(CM)therapy and disease-free survival(DFS)outcomes in postoperative patients with non-small cell lung cancer(NSCLC).Methods:This multiple-center prospectiv...Objective:To evaluate the association between Chinese medicine(CM)therapy and disease-free survival(DFS)outcomes in postoperative patients with non-small cell lung cancer(NSCLC).Methods:This multiple-center prospective cohort study was conducted in 13 medical centers in China.Patients with stage I,II,or IDA NSCLC who had undergone radical resection and received conventional postoperative treatment according to the National Comprehensive Cancer Network(NCCN)guidelines were recruited.The recruited patients were divided into a CM treatment group and a control group according to their wishes.Patients in the CM treatment group received continuous CM therapy for more than 6 months or until disease progression.Patients in the control group received CM therapy for less than 1 month.Follow-up was 8nducted over 3 years.The primary outcome was DFS,with recurrence/metastasis rates as a secondary outcome.Results:Between May 2013 and August 2016,503 patients were enrolled into the cohort;266 were classified in the CM treatment group and 237 in the control group.Adjusting for covariates,high exposure to CM was associated with better DFS[hazard ratio(HR)=0.417,95%confidential interval(Cl):0.307-0.567)].A longer duration of CM therapy(6-12 months,12-18 months,>24 months)was associated with lower recurrence and metastasis rates(HR=0.225,0.119 and 0.083,respectively).In a subgroup exploratory analysis,CM therapy was also a protective factor of cancer recurrence and metastasis in both stage n-MA(HR=0.50,95%Cl:0.37-0.67)and stage IDA NSCLC postoperative patients(HR=0.48,95%Cl:0.33-0.71),DFS was even longer among CM treatment group patients.Conclusions:Lon ger duration of CM therapy could be 8nsidered a protective factor of cancer recurrence and metastasis.CM treatment is associated with improving survival outcomes of postoperative NSCLC patients in China.展开更多
基金Beijing Administration of Traditional Chinese Medicine Major Difficult Diseases Clinical Collaboration Project of Traditional Chinese and Western Medicine (No.2019-ZX-005)。
文摘Objective:To explore the curative effect of nano-Kuiyangye on radiation esophagitis in Wistar rats,and to explore its possible mechanism.Methods:Wistar rats were irradiated locally with 30Gy rays to establish an animal model of radiation esophagitis in rats.After irradiation,nano-Kuiyangye,traditional Chinese medicine ulcer solution,Kangfuxin solution,nano-hydrotalcite matrix,and distilled water were used to intervene continuously for 7 d,during which the body weight and food intake of the rats were recorded.On day 7,blood was collected from the abdominal aorta under anesthesia,and the serum was obtained by centrifugation.The expression levels of pain-related factors prostaglandin-2(PGE-2),substance P(SP),and calcitonin gene-related peptide(CGRP)were detected by ELISA.Rats were sacrificed after blood collection,and full-length esophageal tissues were taken.Hematoxylin-eosin(HE)staining was performed to analyze the pathological changes of the rat esophagus,and Western Blotting was used to detect the expression of nuclear factor-κB(NF-κB)inflammatory protein.Results:Compared with the control group,the total food intake and body weight of the rats within 7 d after modeling were significantly decreased(P<0.05),and the expressions of pain-related factors PGE-2,SP,and CGRP were higher than those of the control group(P<0.05).,the esophageal pathological damage score increased(P<0.05),and the expression of NF-κB inflammatory protein increased(P<0.05);after treatment,the total food intake of the rats in the nano-Kuiyangye intervention group within 7 days after modeling was higher than that in other groups(P<0.05),the expressions of pain-related factors PGE-2,SP,and CGRP were lower than those in the model group(P<0.05),the esophageal pathological damage score was lower(P<0.05),and the expression of NF-κB inflammatory proteins was lower(P<0.05).Conclusion:Nano-Kuiyangye increases the food intake of rats with radiation esophagitis,reduces esophageal tissue damage,and reduces the concentration of serum pain factors;the anti-inflammatory effect of nano-Kuiyangye may be related to the reduction of NF-κB inflammatory factor levels.
文摘目的通过对药物临床试验临床研究协调员(clinical research coordinator,CRC)进行调查,提出建议和优化CRC管理体系,以提高药物临床试验质量。方法于2021年4月6日至4月12日期间通过问卷星向调查对象发放自行设计的问卷进行工作现状调查分析,回收有效问卷137份。结果CRC来源复杂良莠不齐,大部分CRC目前工作强度过大,对参加的培训满意度较低,且严重缺乏归属感。结论医疗机构应建立有效的SMO和CRC的分级量化管理制度,完善人员准入和劝退机制,加强研究者和CRC有效培训,并搭建合作平台进一步提高CRC的工作质量,保障药物临床试验质量。
文摘1文献来源Al-Batran SE,Homann N,Pauligk C,et al.Perioperative chemotherapy with fluorouracil plus leucovorin,oxaliplatin,and docetaxel versus fluorouracil or capecitabine plus cisplatin and epirubicin for locally advanced,resectable gastric or gastro-oesophageal junction adenocarcinoma(FLOT4):A randomised,phase 2/3 trial[J].Lancet,2019,393(10184):1948-1957.
基金Supported by Special Funded Projects of the TCM Industry(No.201307006)National Natural Science Foundation of China(No.81473467)Beijing Natural Science Fund(No.7192181)
文摘Objective:To evaluate the association between Chinese medicine(CM)therapy and disease-free survival(DFS)outcomes in postoperative patients with non-small cell lung cancer(NSCLC).Methods:This multiple-center prospective cohort study was conducted in 13 medical centers in China.Patients with stage I,II,or IDA NSCLC who had undergone radical resection and received conventional postoperative treatment according to the National Comprehensive Cancer Network(NCCN)guidelines were recruited.The recruited patients were divided into a CM treatment group and a control group according to their wishes.Patients in the CM treatment group received continuous CM therapy for more than 6 months or until disease progression.Patients in the control group received CM therapy for less than 1 month.Follow-up was 8nducted over 3 years.The primary outcome was DFS,with recurrence/metastasis rates as a secondary outcome.Results:Between May 2013 and August 2016,503 patients were enrolled into the cohort;266 were classified in the CM treatment group and 237 in the control group.Adjusting for covariates,high exposure to CM was associated with better DFS[hazard ratio(HR)=0.417,95%confidential interval(Cl):0.307-0.567)].A longer duration of CM therapy(6-12 months,12-18 months,>24 months)was associated with lower recurrence and metastasis rates(HR=0.225,0.119 and 0.083,respectively).In a subgroup exploratory analysis,CM therapy was also a protective factor of cancer recurrence and metastasis in both stage n-MA(HR=0.50,95%Cl:0.37-0.67)and stage IDA NSCLC postoperative patients(HR=0.48,95%Cl:0.33-0.71),DFS was even longer among CM treatment group patients.Conclusions:Lon ger duration of CM therapy could be 8nsidered a protective factor of cancer recurrence and metastasis.CM treatment is associated with improving survival outcomes of postoperative NSCLC patients in China.
