The present study carried out a phytochemical investigation of the methanol extract of the branches and leaves of Clausena lansium and afforded nine carbazole alkaloids(compounds 1–9) including two new carbazole alka...The present study carried out a phytochemical investigation of the methanol extract of the branches and leaves of Clausena lansium and afforded nine carbazole alkaloids(compounds 1–9) including two new carbazole alkaloids, claulansiums A and B(compounds 1 and 2). The new compounds were elucidated on the basis of extensive spectroscopic data(MS, NMR, IR, and UV) and the known compounds were identified by comparing spectroscopic data with those reported in literature. All the isolated compounds were tested for their cytotoxic activity against A549 and Hela cancer cell lines. Our results showed that compounds 2–6 exhibited varying degrees of cytotoxicity to cancer cells, with IC_(50) values ranging from 8.67 to 98.89 μmol·L^(-1).展开更多
文摘目的探讨二甲双胍对自然衰老小鼠神经元损伤的保护作用及其作用机制。方法采用4月龄小鼠作为年轻对照组,18月龄小鼠作为老龄鼠,15月龄给予250mg/kg/d二甲双胍灌胃给药3个月作为治疗组,采用苏木精-伊红染色检测脑组织形态学,利用Morris Water Maze方法进行水迷宫实验;利用人神经母细胞瘤细胞SHSY5Y构建神经元氧化应激损伤模型,细胞实验分4组:对照组、H_(2)O_(2)组、H_(2)O_(2)+二甲双胍组与H_(2)O_(2)+二甲双胍+Compound C组;采用蛋白质免疫印迹法检测自噬及凋亡相关蛋白的表达。结果 18月龄小鼠学习记忆能力与年轻组相比显著下降,自噬水平降低,凋亡增加,经二甲双治疗后有所缓解;神经元细胞在受到刺激损伤后,自噬相关蛋白Beclin1、LC3Ⅱ/Ⅰ减少,凋亡增加;给予二甲双胍治疗后,腺苷单磷酸活化蛋白激酶(adenosine monophosphate-activated protein kinase, AMPK)被激活,自噬水平升高,凋亡相关蛋白Bax/Bcl-2比值降低;给予AMPK抑制剂Compound C后自噬水平没有明显变化。结论二甲双胍通过激活AMPK引起自噬水平升高,进而保护H_(2)O_(2)诱导的神经元细胞免于凋亡和衰老相关神经退行性病变。
基金supported by the National Natural Science Foundation of China(No.31660094)
文摘The present study carried out a phytochemical investigation of the methanol extract of the branches and leaves of Clausena lansium and afforded nine carbazole alkaloids(compounds 1–9) including two new carbazole alkaloids, claulansiums A and B(compounds 1 and 2). The new compounds were elucidated on the basis of extensive spectroscopic data(MS, NMR, IR, and UV) and the known compounds were identified by comparing spectroscopic data with those reported in literature. All the isolated compounds were tested for their cytotoxic activity against A549 and Hela cancer cell lines. Our results showed that compounds 2–6 exhibited varying degrees of cytotoxicity to cancer cells, with IC_(50) values ranging from 8.67 to 98.89 μmol·L^(-1).
