目的:探讨加速康复外科(enhanced recovery after surgery,ERAS)理念在儿童发育性髋关节发育不良(developmental dysplasia of the hip,DDH)围手术期管理中的应用价值。方法:将60例DDH患儿随机分为2组,每组30例。2组患儿均采用骨盆截骨...目的:探讨加速康复外科(enhanced recovery after surgery,ERAS)理念在儿童发育性髋关节发育不良(developmental dysplasia of the hip,DDH)围手术期管理中的应用价值。方法:将60例DDH患儿随机分为2组,每组30例。2组患儿均采用骨盆截骨术联合股骨近端截骨术治疗。分别在ERAS理念指导下(ERAS组)和传统康复理念指导下(传统康复组)进行围手术期管理。记录并比较2组患儿的手术时间、术中失血量、术中输血情况、住院时间、髋关节疼痛视觉模拟量表(visual analogue scale,VAS)评分、切口周围肿胀程度、切口周围肿胀消退时间及并发症发生情况。结果:①一般结果。2组患儿手术时间、术中失血量及术中输血情况比较,组间差异均无统计学意义[(107.70±6.92)min,(107.47±6.91)min,t=0.131,P=0.896;(180.00±48.87)mL,(190.83±48.60)mL,t=0.861,P=0.393;χ^(2)=0.647,P=0.421];ERAS组患儿住院时间短于传统康复组[(7.57±1.65)d,(10.36±1.56)d,t=6.720,P=0.000]。②髋关节疼痛VAS评分。时间因素与分组因素存在交互效应(F=3.220,P=0.047);2组患儿髋关节疼痛VAS评分总体比较,组间差异有统计学意义,即存在分组效应(F=33.928,P=0.000);术后不同时间点髋关节疼痛VAS评分的差异有统计学意义,即存在时间效应(F=108.156,P=0.000);2组患儿髋关节疼痛VAS评分随时间变化均呈下降趋势,但2组的下降趋势不完全一致[(3.23±1.57)分,(2.17±1.15)分,(1.60±0.72)分,F=74.242,P=0.000;(4.57±1.07)分,(3.83±1.21)分,(3.32±0.87)分,F=37.134,P=0.000];术后6h、12h、24h,ERAS组髋关节疼痛VAS评分均低于传统康复组(t=14.768,P=0.000;t=30.083,P=0.000;t=77.440,P=0.000)。③切口周围肿胀情况。ERAS组患儿术后24h切口肿胀Ⅰ级22例、Ⅱ级8例,传统康复组术后24h切口肿胀Ⅰ级14例、Ⅱ级16例。ERAS组患儿术后24h切口周围肿胀程度低于传统康复组(χ^(2)=4.444,P=0.035),切口周围肿胀消退时间短于传统康复组[(3.50±1.11)d,(5.43±1.14)d,t=6.680,P=0.000]。④并发症发生情况。ERAS组术后3例发生早期发热、2例发生恶心呕吐、2例发生腹痛腹胀,传统康复组术后8例发生早期发热、4例发生恶心呕吐、3例发生腹痛腹胀。2组患儿均未发生切口感染、尿路感染等并发症。ERAS组患儿并发症发生率低于传统康复组(χ^(2)=4.593,P=0.032)。结论:在儿童DDH围手术期管理中应用ERAS理念,能够缩短住院时间、缓解髋关节疼痛及切口周围肿胀,提高手术安全性。展开更多
The nucleus of a somatic cell could be dedif-ferentiated and reprogrammed in an enucleated heterogeneous oocyte. Some reconstructed oocytes could develop into blastocysts in vitro, and a few could develop into term no...The nucleus of a somatic cell could be dedif-ferentiated and reprogrammed in an enucleated heterogeneous oocyte. Some reconstructed oocytes could develop into blastocysts in vitro, and a few could develop into term normally after transferred into foster mothers, but most of cloning embryos fail to develop to term. In order to evaluate the efficacy of embryonic stem cell as nucleus donor in interspecific animal cloning, we reconstructed enucleated rabbit oocytes with nuclei from mouse ES cells, and analyzed the developmental ability of reconstructed embryos in vitro. Two kinds of fibroblast cells were used as donor control, one derived from ear skin of an adult Kunming albino mouse, and the other derived from a mouse fetus. Three types of cells were transferred into perivitelline space under zona pellu-cida of rabbit oocytes respectively. The reconstructed oocytes were fused and activated by electric pulses, and cultured in vitro. The developmental rate of reconstructed oocytes derived from embryonic展开更多
文摘目的:探讨加速康复外科(enhanced recovery after surgery,ERAS)理念在儿童发育性髋关节发育不良(developmental dysplasia of the hip,DDH)围手术期管理中的应用价值。方法:将60例DDH患儿随机分为2组,每组30例。2组患儿均采用骨盆截骨术联合股骨近端截骨术治疗。分别在ERAS理念指导下(ERAS组)和传统康复理念指导下(传统康复组)进行围手术期管理。记录并比较2组患儿的手术时间、术中失血量、术中输血情况、住院时间、髋关节疼痛视觉模拟量表(visual analogue scale,VAS)评分、切口周围肿胀程度、切口周围肿胀消退时间及并发症发生情况。结果:①一般结果。2组患儿手术时间、术中失血量及术中输血情况比较,组间差异均无统计学意义[(107.70±6.92)min,(107.47±6.91)min,t=0.131,P=0.896;(180.00±48.87)mL,(190.83±48.60)mL,t=0.861,P=0.393;χ^(2)=0.647,P=0.421];ERAS组患儿住院时间短于传统康复组[(7.57±1.65)d,(10.36±1.56)d,t=6.720,P=0.000]。②髋关节疼痛VAS评分。时间因素与分组因素存在交互效应(F=3.220,P=0.047);2组患儿髋关节疼痛VAS评分总体比较,组间差异有统计学意义,即存在分组效应(F=33.928,P=0.000);术后不同时间点髋关节疼痛VAS评分的差异有统计学意义,即存在时间效应(F=108.156,P=0.000);2组患儿髋关节疼痛VAS评分随时间变化均呈下降趋势,但2组的下降趋势不完全一致[(3.23±1.57)分,(2.17±1.15)分,(1.60±0.72)分,F=74.242,P=0.000;(4.57±1.07)分,(3.83±1.21)分,(3.32±0.87)分,F=37.134,P=0.000];术后6h、12h、24h,ERAS组髋关节疼痛VAS评分均低于传统康复组(t=14.768,P=0.000;t=30.083,P=0.000;t=77.440,P=0.000)。③切口周围肿胀情况。ERAS组患儿术后24h切口肿胀Ⅰ级22例、Ⅱ级8例,传统康复组术后24h切口肿胀Ⅰ级14例、Ⅱ级16例。ERAS组患儿术后24h切口周围肿胀程度低于传统康复组(χ^(2)=4.444,P=0.035),切口周围肿胀消退时间短于传统康复组[(3.50±1.11)d,(5.43±1.14)d,t=6.680,P=0.000]。④并发症发生情况。ERAS组术后3例发生早期发热、2例发生恶心呕吐、2例发生腹痛腹胀,传统康复组术后8例发生早期发热、4例发生恶心呕吐、3例发生腹痛腹胀。2组患儿均未发生切口感染、尿路感染等并发症。ERAS组患儿并发症发生率低于传统康复组(χ^(2)=4.593,P=0.032)。结论:在儿童DDH围手术期管理中应用ERAS理念,能够缩短住院时间、缓解髋关节疼痛及切口周围肿胀,提高手术安全性。
基金This work was supported by the fund of Climbing Program of the Ministry of Science and Technology (Grant No. 95-specific-08) and the Chinese Academy of Sciences (Grant No. KSCX1-05-01).
文摘The nucleus of a somatic cell could be dedif-ferentiated and reprogrammed in an enucleated heterogeneous oocyte. Some reconstructed oocytes could develop into blastocysts in vitro, and a few could develop into term normally after transferred into foster mothers, but most of cloning embryos fail to develop to term. In order to evaluate the efficacy of embryonic stem cell as nucleus donor in interspecific animal cloning, we reconstructed enucleated rabbit oocytes with nuclei from mouse ES cells, and analyzed the developmental ability of reconstructed embryos in vitro. Two kinds of fibroblast cells were used as donor control, one derived from ear skin of an adult Kunming albino mouse, and the other derived from a mouse fetus. Three types of cells were transferred into perivitelline space under zona pellu-cida of rabbit oocytes respectively. The reconstructed oocytes were fused and activated by electric pulses, and cultured in vitro. The developmental rate of reconstructed oocytes derived from embryonic