目的建立超高效液相色谱-串联三重四极杆质谱法(ultra performance liquid chromatography-tandem with triple quadrupole mass spectrometry,UPLC-MS/MS)同时测定谷物及其制品中的10种真菌毒素的检测方法。方法以0.1%甲酸-80%乙腈水...目的建立超高效液相色谱-串联三重四极杆质谱法(ultra performance liquid chromatography-tandem with triple quadrupole mass spectrometry,UPLC-MS/MS)同时测定谷物及其制品中的10种真菌毒素的检测方法。方法以0.1%甲酸-80%乙腈水为提取溶剂,经过涡旋、振荡提取后,加入Supel QuE z-sep+作为净化剂进行初级净化,离心后的上清液过PRiME HLB固相萃取柱二次净化,经UPLC-MS/MS测定,质谱采用多反应监测模式定性,基质匹配标准溶液外标法定量。结果10种真菌毒素在谷物中的检出限为0.10~10.00μg/kg,定量限为0.35~20.00μg/kg。在玉米、花生、大米3种谷物基质中的回收率为73.2%~98.1%,相对标准偏差为1.3%~9.3%。结论该方法的灵敏度满足国家标准对真菌毒素的限量要求,实验操作简便快速,成本较低,定性定量分析可靠,适合谷物中真菌毒素的多组分高通量快速分析测定。展开更多
A steady-state,direct-current high-pressure CH_(4)-H_(2) glow discharge in a cup-shaped cathode parallel to anode configuration is investigated by using their V–I characteristics and CCD images.The discharges display...A steady-state,direct-current high-pressure CH_(4)-H_(2) glow discharge in a cup-shaped cathode parallel to anode configuration is investigated by using their V–I characteristics and CCD images.The discharges display an abnormal glow feature,and an expansion of a negative glow is observed on the cathode sidewall with the increasing discharge current.There exists a dependence of voltage on gas pressure for different fixed currents.The voltage decreases with gas pressure initially,and then increases conversely,which is correlated with the glow states of the cathode sidewall.This study exhibits a self-adjusting characterization for plasmas in cathode fall,which is important for maintaining steady-state,abnormal glow discharge in a relatively high pressure range.展开更多
文摘目的建立超高效液相色谱-串联三重四极杆质谱法(ultra performance liquid chromatography-tandem with triple quadrupole mass spectrometry,UPLC-MS/MS)同时测定谷物及其制品中的10种真菌毒素的检测方法。方法以0.1%甲酸-80%乙腈水为提取溶剂,经过涡旋、振荡提取后,加入Supel QuE z-sep+作为净化剂进行初级净化,离心后的上清液过PRiME HLB固相萃取柱二次净化,经UPLC-MS/MS测定,质谱采用多反应监测模式定性,基质匹配标准溶液外标法定量。结果10种真菌毒素在谷物中的检出限为0.10~10.00μg/kg,定量限为0.35~20.00μg/kg。在玉米、花生、大米3种谷物基质中的回收率为73.2%~98.1%,相对标准偏差为1.3%~9.3%。结论该方法的灵敏度满足国家标准对真菌毒素的限量要求,实验操作简便快速,成本较低,定性定量分析可靠,适合谷物中真菌毒素的多组分高通量快速分析测定。
基金Supported by the National Natural Science Foundation of China under Grant No 11075158the Fund for Fostering Talents in Basic Science of the National Natural Science Foundation of China(No J1103207)。
文摘A steady-state,direct-current high-pressure CH_(4)-H_(2) glow discharge in a cup-shaped cathode parallel to anode configuration is investigated by using their V–I characteristics and CCD images.The discharges display an abnormal glow feature,and an expansion of a negative glow is observed on the cathode sidewall with the increasing discharge current.There exists a dependence of voltage on gas pressure for different fixed currents.The voltage decreases with gas pressure initially,and then increases conversely,which is correlated with the glow states of the cathode sidewall.This study exhibits a self-adjusting characterization for plasmas in cathode fall,which is important for maintaining steady-state,abnormal glow discharge in a relatively high pressure range.
