OBJECTIVE:To explore the functional role of the drugdependent mesenchymal-epithelial transition(Met)-axiation"π"structural module of neurogenesis after processing by three components of Qingkailing injectio...OBJECTIVE:To explore the functional role of the drugdependent mesenchymal-epithelial transition(Met)-axiation"π"structural module of neurogenesis after processing by three components of Qingkailing injection in neurogenesis and angiogenesis in cerebral ischemia.METHODS:We used a Glutathione S-transferase(GST)-pull down assay,isothermal titration calorimetry assay,and other related methods to identify the relationships among Met,inositol polyphosphate phosphatase like 1(Inppl1),and death associated protein kinase 3(Dapk3)in this allosteric module.The biological effects of the modules of neurons generation composed of Met,Inppl1,and Dapk3 were measured through Western blot,apoptosis analysis,and double immunofluorescence labeling.RESULTS:The GST-pull down assay revealed that proline-serine-threonine rich domain of Met binds to the Src homology domain of Inppl1 to form a protein-protein complex;Dapk3 with a C-terminal domain interacts weakly with the protein kinase C domain of Met in the intracellular region.Thus,we obtained a“π”structuring module considered a neural regeneration module.The biological effects of angiogenesis and neurogenesis modules composed of Met,Inppl1,and Dapk3 were also verified.CONCLUSION:The study suggested that understanding the functional modules that contribute to pharmaceutics might provide novel signatures that can be used as endpoints to define disease processes under stroke or cerebral ischemia conditions.展开更多
The development of wireless monitoring is currently restricted by the short lifetime of batteries,requiring frequent replacement.Utilization of abundant mechanical energy from the surrounding environment has attracted...The development of wireless monitoring is currently restricted by the short lifetime of batteries,requiring frequent replacement.Utilization of abundant mechanical energy from the surrounding environment has attracted increasing attention in real-time monitoring.Herein,a coil-levitated hybrid generator was developed for the efficient harvesting of mechanical energy from mechanical motion.The novel coil-levitated structure adapted to the metal and magnetic environment.The output currents were systematically analyzed at different operation modes based on the unique combination of triboelectrification,electromagnetic induction,and piezoelectric effect.Under the excitation of vibration frequency and amplitude of 8 Hz and 5 mm respectively,the as-constructed triboelectric nanogenerator delivered a peak power density of 11.40 W/m3 at 10 MΩ.Meanwhile,the middle electromagnetic part and bottom piezoelectric generator provided peak power densities of 6.97 and 79.93 W/m2 at 10000Ω,respectively.More importantly,the battery charging experiment was verified,in which a 30 mA h Li-ion battery can be charged from 2.57 to 3.27 V in about 90 min.In sum,a self-powered temperature and vibration monitoring system was successfully developed based on hybrid generator,promising for realizing wireless monitoring of mechanical equipment without any external power supply.展开更多
基金National Major Scientific and Technological Special Project for“Significant New Drugs Development”:Clinical Value Prescription Discovery and Evaluation Technology Based on Modular Pharmacology(No.2017ZX09301059)the Joint Innovation Project of China Academy of Chinese Medical Sciences:Study on the Mechanism of Action of Neuronal Regenerative Function Module Activated by Qingkailing Components(No.ZZ11-026)+1 种基金the University Collaborative Innovation Project of Anhui:Creation of a Combined Animal Model of Coronary Heart Disease Based on the Theory of Xin'an Medicine(No.GXXT-2020-024)the University Natural Science Research Project of Anhui Province:Molecular Mechanism of the Anti-hepatic Fibrosis Effect of Wickerwork Amide Based on the Regulation of Intercellular Communication by PTRF(No.2023AH051752)。
文摘OBJECTIVE:To explore the functional role of the drugdependent mesenchymal-epithelial transition(Met)-axiation"π"structural module of neurogenesis after processing by three components of Qingkailing injection in neurogenesis and angiogenesis in cerebral ischemia.METHODS:We used a Glutathione S-transferase(GST)-pull down assay,isothermal titration calorimetry assay,and other related methods to identify the relationships among Met,inositol polyphosphate phosphatase like 1(Inppl1),and death associated protein kinase 3(Dapk3)in this allosteric module.The biological effects of the modules of neurons generation composed of Met,Inppl1,and Dapk3 were measured through Western blot,apoptosis analysis,and double immunofluorescence labeling.RESULTS:The GST-pull down assay revealed that proline-serine-threonine rich domain of Met binds to the Src homology domain of Inppl1 to form a protein-protein complex;Dapk3 with a C-terminal domain interacts weakly with the protein kinase C domain of Met in the intracellular region.Thus,we obtained a“π”structuring module considered a neural regeneration module.The biological effects of angiogenesis and neurogenesis modules composed of Met,Inppl1,and Dapk3 were also verified.CONCLUSION:The study suggested that understanding the functional modules that contribute to pharmaceutics might provide novel signatures that can be used as endpoints to define disease processes under stroke or cerebral ischemia conditions.
基金the National Key Research and Development Program of China(Grant No.2019YFB2004800)the National Natural Science Foundation for Distinguished Young Scholars of China(Grant No.61525107)+1 种基金the National Natural Science Foundation for China as National Major Scientific Instruments Development Project(Grant No.61727806)the Fund for Key Laboratory of Equipment Pre-research(Grant No.6142001190409)。
文摘The development of wireless monitoring is currently restricted by the short lifetime of batteries,requiring frequent replacement.Utilization of abundant mechanical energy from the surrounding environment has attracted increasing attention in real-time monitoring.Herein,a coil-levitated hybrid generator was developed for the efficient harvesting of mechanical energy from mechanical motion.The novel coil-levitated structure adapted to the metal and magnetic environment.The output currents were systematically analyzed at different operation modes based on the unique combination of triboelectrification,electromagnetic induction,and piezoelectric effect.Under the excitation of vibration frequency and amplitude of 8 Hz and 5 mm respectively,the as-constructed triboelectric nanogenerator delivered a peak power density of 11.40 W/m3 at 10 MΩ.Meanwhile,the middle electromagnetic part and bottom piezoelectric generator provided peak power densities of 6.97 and 79.93 W/m2 at 10000Ω,respectively.More importantly,the battery charging experiment was verified,in which a 30 mA h Li-ion battery can be charged from 2.57 to 3.27 V in about 90 min.In sum,a self-powered temperature and vibration monitoring system was successfully developed based on hybrid generator,promising for realizing wireless monitoring of mechanical equipment without any external power supply.
