A novel preparation method was developed to form a surfactant-lipase complex for the resolution of 2-octanol in a solvent-free system. The E value was improved from 6.64 to 120.2. The lipase modified by the anion...A novel preparation method was developed to form a surfactant-lipase complex for the resolution of 2-octanol in a solvent-free system. The E value was improved from 6.64 to 120.2. The lipase modified by the anionic surfactant possessed a low solubility in the solvent-free system, which was beneficial to the recovery and the repeated usage of the lipase. The surfactant-lipase complex maintained a high enantioselectivity after five cycles of usage. The effect of water on both the activity and the enantioselectivity of the lipase has also been investigated; the direct addition of a salt hydrate pair of Na 4P 2O 7·H 2O and Na 4P 2O 7 can dramatically activate the modified enzyme.展开更多
基金Supported by the National Natural Science Foundation of China( No.2 0 0 72 0 11and 2 0 2 72 0 17)
文摘A novel preparation method was developed to form a surfactant-lipase complex for the resolution of 2-octanol in a solvent-free system. The E value was improved from 6.64 to 120.2. The lipase modified by the anionic surfactant possessed a low solubility in the solvent-free system, which was beneficial to the recovery and the repeated usage of the lipase. The surfactant-lipase complex maintained a high enantioselectivity after five cycles of usage. The effect of water on both the activity and the enantioselectivity of the lipase has also been investigated; the direct addition of a salt hydrate pair of Na 4P 2O 7·H 2O and Na 4P 2O 7 can dramatically activate the modified enzyme.
