Silencing of peroxiredoxin 2 suppresses proliferation and Wnt/β-catenin pathway,and induces senescence in hepatocellular carcinoma

Hepatocellular carcinoma(HCC),a common malignancy worldwide,still lacks effective clinical treatment.The study aimed to investigate the oncogenes that affect the progression of HCC and their possible mechanisms.In our study,we initially confirmed a higher level of PRDX2 in the bile of HCC patients compared to those with choledocholithiasis by 2-DE,LC-MS,and ELISA.Subsequently,we demonstrated the high expression of peroxiredoxin 2(PRDX2)in HCC based on the TCGA database and clinical sample analysis.Furthermore,PRDX2 overexpression enhanced the viability of HCC cells.And PRDX2 silencing induced senescence of HCC cells.In vivo,knockdown of PRDX2 significantly reduced the weight of xenograft tumors.PRDX2 also was found to activate the Wnt/β-catenin pathway by inducingβ-catenin nuclear translocation.Consequently,we proved that silencing PRDX2 could inhibit proliferation and Wnt/β-catenin pathway while promoting senescence in HCC cells.

FXR调控胆汁酸与糖脂代谢改善NAFLD的机制

非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)是慢性肝病的主要病因,以肝脏代谢紊乱为主要改变,体现为脂质异常蓄积、肝细胞氧化应激等,其病因尚不明确.“肠肝轴”中法尼醇X受体(farnesoid X receptor,FXR)是一种主要的胆汁酸受体,在“肠-肝轴”的基础上,通过不同通路调节体内多种物质代谢和病理生理状态,进而影响NAFLD的发生发展,是一个具有潜力的治疗靶点.本文主要对FXR通过“肠-肝轴”调控体内胆汁酸与糖脂代谢进而改善NAFLD的机制进行综述,以为后续关于FXR靶点药物的研究提供思路.

Evaluation of Ultrasound-guided Genitofemoral Nerve Block Combined with Ilioinguinal/iliohypogastric Nerve Block during Inguinal Hernia Repair in the Elderly

To evaluate the anesthetic effect of ultrasound-guided(USG)ilioinguinal/iliohypogastric nerve(Ⅱ/IHN)block combined with genital branch of genitofemoral nerve(GFN)block in the elderly undergoing inguinal hernia repair,54 old patients(aged 60-96years,ASAⅠ-Ⅲ)with indirect hernia were enrolled and scheduled for unilateral tensiofree herniorrhaphy.Patients were grouped randomly to receive either USGⅡ/IHN plus GFN block(Group G)or USGⅡ/IHN block alone(GroupⅠ).The intraoperative visual analogue scale(VAS)scores were recorded at skin incision,at spermatic cord/round ligament traction and at sac ligation.The resting and dynamic VAS scores were recorded postoperatively.The requirements of extra sedatives and analgesics for intra-and postoperative analgesia were assessed.Occurrence of complications of the block,postoperative nausea and vomiting and femoral nerve palsy was also reported.Both groups showed similar sensory block.When stretching spermatic cord/round ligament,the patients in group G had significantly lower VAS scores than in group.And group G used much fewer adjuvant sedatives and analgesics to achieve adequate anaesthesia.In addition,group G was presented with better intraoperative anaesthesia and lower postoperative dynamic VAS scores at all time points tested.No significant difference was found in the postoperative requirement of rescue medication.Both groups showed no complications related to the block and group G reported no femoral nerve palsy.The addition of GFN block toⅡ/IHN block improves the quality of perioperative anesthesia and analgesia in the elderly and reduces the consumption of extra sedatives and analgesics during the surgery.

Effectiveness of sharp recanalization of superior vena cava-right atrium junction occlusion

BACKGROUND Conventional recanalization techniques may fail in patients with completely occluded superior vena cava(SVC).AIM To analyze the effectiveness and complications of sharp recanalization for completely occluded SVC.METHODS This was a retrospective study of patients that underwent puncture and recanalization of the SVC between January 2016 and December 2017 at our hospital.Sharp recanalization was performed using the RUPS-100 system.The patients were followed for 12 mo.The main outcomes were the patency rate of SVC and arteriovenous fistula flow during dialysis.RESULTS The procedure was successful in all 14 patients(100%).Blood pressure in the distal SVC decreased in all 14 cases(100%)from 26.4±2.7 cmH2O to 14.7±1.3 cmH2O(P<0.05).The first patency rates of the SVC at 24 h and at 3,6,9 and 12 mo after sharp recanalization were 100%,92.9%,85.7%,78.6%and 71.4%,respectively.There were two(14.3%)severe,one(7.1%)moderate and one(7.1%)minor complication.The severe complications included one case of pericardial tamponade and one case of hemothorax.CONCLUSION The results suggest that sharp recanalization can be an additional tool to extend or renew the use of an occluded upper extremity access for hemodialysis.This could be of use in patients with long-term maintenance hemodialysis in whom the maintenance of central venous access is often a challenge.

Inflatable hollow obturator prostheses for patients undergoing an extensive maxillectomy:a case report

The presence of a large palatal or maxillary defect after partial or total maxillectomy for tumor, trauma or congenital deformation poses a challenge to prosthodontists, particularly when the use of an implant cannot be considered. This case report described the use of an air valve in a hollow silicone obturator to manufacture an inflatable obturator that could be extended further into undercut area to retain itself. The inflatable obturator exhibited adequate retention, stability and border sealing, thereby improving the masticatory, pronunciation and swallowing functions of patients. It may be a suitable alternative treatment option to an implant-retained obturator.

Absolute Orientation of Molecules with Competing Hydrophilic Head Groups at the Air/Water Interface Probed with Sum Frequency Generation Vibrational Spectroscopy

建设性或破坏光谱在在在和频率产生(SFG ) 的接口上下面向的分子的组之间的干扰系列提供这些分子的组的绝对取向的直接大小。这条简单途径能被采用讯问除在 SFG 研究使用复杂绝对阶段测量以外的绝对分子的取向。我们在 p-cyanophenol ( PCP )使用了 CN 组是的分子内部阶段标准，并且我们由测量 PCP ， 35DMHBN ，和 26DMHBN 解决方案的混合物的水的表面的 SFG 系列在空气/水接口在 3,5-dimethyl-4-hydroxy-benzonitrile ( 35DMHBN )和 2,6-dimethyl-4-hydroxy-benzonitrile ( 26DMHBN )测量了 CN 组的 SFG 领域的阶段。结果证明 35DMHBN 让它的 CN 组指进水的阶段；当时 26DMHBN，类似于 PCP，让它的 CN 组指离开水的阶段。为在空气 / 水接口的 35DMHBN 和 26DMHBN 分子的 CN 组的 tilt 角度在 25 °附近是从接口正常的 45 °。这些结果在竞争因素的详细平衡的理解上提供了卓见，例如极的头组，氢结合和恐水病的效果的 solvation，等等，在在空气 / 水接口影响绝对分子的取向上。

Growth of Ag/g-C_(3)N_(4) nanocomposites on nickel foam to enhance photocatalytic degradation of formaldehyde under visible light

Formaldehyde is a pollutant that significantly affects the indoor air quality.However,conventional remediation approaches can be challenging to deal with low-concentration formaldehyde in an indoor environment.In this study,Photocatalysts of Ag/graphitic carbon nitride(g-C_(3)N_(4))/Ni with 3D reticulated coral structure were prepared by thermal polymerization and liquid phase photo-deposition,using nickel foam(NF)as the carrier.Experiments demonstrated that when the Ag concentration was 3%,and the relative humidity was 60%,the Ni/Ag/g-C_(3)N_(4)showed the maximum degradation rate of formaldehyde at 90.19%under visible light irradiation,and the formaldehyde concentration after degradation was lower than the Hygienic standard stated by the Chinese Government.The porous structure of Ni/Ag/g-C_(3)N_(4)and the formation of Schottky junctions promoted the Adsorption efficiency and degradation of formaldehyde,while the nickel foam carrier effectively promoted the desorption of degradation products.Meanwhile,the degradation rate was only reduced by3.4%after 16 recycles,the three-dimensional porous structure extended the lifetime of the photocatalyst.This study provides a new strategy for the degradation of indoor formaldehyde at low concentrations.

Recent progress in co-detection of single-cell transcripts and proteins

Cellular heterogeneity is a universal property of living systems,and the interrogation of single cells facilitates in-depth understanding of distinct cellular states and functions in various biological processes.Co-analysis of transcripts and proteins from the same single cells opens the way to decipher complex RNA regulatory frameworks and phenotypes,facilitating the understanding of cellular fate and function regulations,discovery of novel cell types,and construction of a high-resolution cell atlas.Herein,we review the state-of-art advances in the development of methodologies for co-analysis of single-cell transcripts and proteins.First,imaging-based methods are summarized with particular emphasis on optical and mass spectrometry imaging.Next,sequencing-based approaches for high-throughput and sensitive co-analysis of single-cell transcripts and proteins are described,including droplet-,microwell-,and split-pool-based platforms.Subsequently,combined methods with more flexibility and universality are discussed.These methods commonly employ different strategies or reactions to convert transcripts and proteins of single cells into distinct signals simultaneously,which can be detected by different instruments or platforms.Lastly,some perspectives on the future challenges and development trends in this field are presented.

VT3D:a visualization toolbox for 3D transcriptomic data

Data visualization empowers researchers to communicate their results that support scientific reasoning in an intuitive way.Three-dimension(3D)spatially resolved transcriptomic atlases constructed from multi-view and high-dimensional data have rapidly emerged as a powerful tool to unravel spatial gene expression patterns and cell type distribution in biological samples,revolutionizing the understanding of gene regulatory interactions and cell niches.However,limited accessible tools for data visualization impede the potential impact and application of this technology.Here we introduce VT3D,a visualization toolbox that allows users to explore 3D transcriptomic data,enabling gene expression projection to any 2D plane of interest,2D virtual slice creation and visualization,and interactive 3D data browsing with surface model plots.In addition,it can either work on personal devices in standalone mode or be hosted as a web-based server.We apply VT3D to multiple datasets produced by the most popular techniques,including both sequencing-based approaches(Stereo-seq,spatial transcriptomics,and Slide-seq)and imaging-based approaches(MERFISH and STARMap),and successfully build a 3D atlas database that allows interactive data browsing.We demonstrate that VT3D bridges the gap between researchers and spatially resolved transcriptomics,thus accelerating related studies such as embryogenesis and organogenesis processes.The source code of VT3D is available at https://github.com/BGI-Qingdao/VT3D,and the modeled atlas database is available at http://www.bgiocean.com/vt3d_example.

基于分层注意力网络的社交媒体谣言检测

在社交媒体谣言检测问题上,现有的基于特征表示学习的研究工作大多数先把微博事件划分为若干个时间段,再对每个时间段提取文本向量表示、全局用户特征等,忽略了时间段内各微博间的时序信息,且未利用到在传统机器学习方法中已取得较好效果的文本潜在信息和局部用户信息,导致性能较低.因此,本文提出了一种基于分层注意力网络的社交媒体谣言检测方法.该方法首先将微博事件按照时间段进行分割,并输入带有注意力机制的双向GRU网络,获取时间段内微博序列的隐层表示,以刻画时间段内微博间的时序信息;然后将每个时间段内的微博视为一个整体,提取文本潜在特征和局部用户特征,并与微博序列的隐层表示相连接,以融入文本潜在信息和局部用户信息;最后通过带有注意力机制的双向GRU网络,得到时间段序列的隐层表示,进而对微博事件进行分类.实验采用了新浪微博数据集和Twitter数据集,实验结果表明,与目前最好的基准方法相比,该方法在新浪微博数据集和Twitter数据集上正确率分别提高了1.5%和1.4%,很好地验证了该方法在社交媒体谣言检测问题上的有效性.

Cancer stem cells in glioblastoma—molecular signaling and therapeutic targeting

Glioblastomas(GBMs)are highly lethal primary brain tumors.Despite current therapeutic advances in other solid cancers,the treatment of these malignant gliomas remains essentially palliative.GBMs are extremely resistant to conventional radiation and chemotherapies.We and others have demonstrated that a highly tumorigenic subpopulation of cancer cells called GBM stem cells(GSCs)promotes therapeutic resistance.We also found that GSCs stimulate tumor angiogenesis by expressing elevated levels of VEGF and contribute to tumor growth,which has been translated into a useful therapeutic strategy in the treatment of recurrent or progressive GBMs.Furthermore,stem cell-like cancer cells(cancer stem cells)have been shown to promote metastasis.Although GBMs rarely metastasize beyond the central nervous system,these highly infiltrative cancers often invade into normal brain tissues preventing surgical resection,and GSCs display an aggressive invasive phenotype.These studies suggest that targeting GSCs may effectively reduce tumor recurrence and significantly improve GBM treatment.Recent studies indicate that cancer stem cells share core signaling pathways with normal somatic or embryonic stem cells,but also display critical distinctions that provide important clues into useful therapeutic targets.In this review,we summarize the current understanding and advances in glioma stem cell research,and discuss potential targeting strategies for future development of anti-GSC therapies.

A novel application of the SAWD-Sabatier-SPE integrated system for CO_2 removal and O_2 regeneration in submarine cabins during prolonged voyages

To improve the working and living environment of submarine crews, an integrated system of CO2 removal and O2 regeneration was designed to work under experimental conditions for 50 people in a submarine cabin during prolonged voyages. The integrated system comprises a solid amine water desorption (SAWD) unit for CO2 collection and concentration, a Sabatier reactor for CO2 reduction and a solid polymer electrolyte (SPE) unit for O2 regeneration by electrolysis. The performances of the SAWD-Sabatier-SPE integrated system were investigated. The experimental results from the SAWD unit showed that the average CO2 concentration in the CO2 storage tank was more than 96% and the outlet CO2 concentration was nearly zero in the first 45 min, and less than 1/10 of inlet CO2 after 60 min when input CO2 was 0.5% (1000 L). About 950 L of CO2 was recovered with a recovery rate of 92%～97%. The output CO2 concentration was less than 0.2%, which showed that the adsorption-desorption performance of this unit was excellent. In the CO2 reduction unit we investigated mainly the start-up and reaction performance of the Sabatier reactor. The start-up time of the Sabatier reactor was 6, 8 and 10 min when the start-up temperature was 187.3, 179.5 and 168 °C, respectively. The product water was colorless, transparent, and had a pH of 6.9～7.5, and an electrical conductivity of 80 μs/cm. The sum of the concentration of metal ions (Ru3+, Al3+, Pb2+) was 0.028% and that of nonmetal ions (Cl-, SO42-) was 0.05%. In the O2 regeneration unit, the O2 generation rate was 0.48 m3/d and the quantity was 2400 L, sufficient to meet the submariners' basic oxygen demands. These results may be useful as a basis for establishing CO2-level limits and O2 regeneration systems in submarines or similar enclosed compartments during prolonged voyages.

Novel theranostic nanoagent based on CuMo_(2)S_(3)-PEG-Gd for MRI-guided photothermal/photodynamic/chemodynamic therapy

Cancer is a severe disease,which have troubled human being for a long time.The development of nanotechnology has provided a new way for cancer treatment.It is a promising strategy to integrate imaging and therapeutic functions into one single nanoplatform to achieve efficient combination of diagnosis and treatment.Herein,we exploited novel CuMo_(2)S_(3)-PEG-Gd nanocomposites(NCs)for magnetic resonance imaging(MRI),guiding the photothermal therapy(PTT)/photodynamic therapy(PDT)/chemodynamic therapy(CDT).The experimental results showed that CuMo_(2)S_(3)-PEG-Gd NCs have a high photothermal conversion efficiency(40.6%),excellent biocompatibility and good biosecurity.The CuMo_(2)S_(3)-PEGGd NCs exhibited a clear MRI performance for tumor due to connecting Gd,which can guide in vivo therapy to improve the therapeutic effect.Moreover,both in vitro and in vivo therapeutic results of CuMo_(2)S_(3)-PEG-Gd NCs exhibited that the PTT/PDT/CDT achieved a remarkably synergistic effect,which could efficiently inhibit the tumor growth.Thus,CuMo_(2)S_(3)-PEG-Gd NCs,which integrated imaging with multiple therapies,have a good potential as theranostic agent for tumor.

Monolithic integration of MoS2-based visible detectors and GaN-based UV detectors

With the increasing demand for high integration and multi-color photodetection for both military and civilian applications, the research of multi-wavelength detectors has become a new research hotspot. However, current research has been mainly in visible dual-or multi-wavelength detectors, while integration of both visible light and ultraviolet(UV) dual-wavelength detectors has rarely been studied. In this work, large-scale and high-quality monolayer MoS2 was grown by the chemical vapor deposition method on transparent free-standing GaN substrate. Monolithic integration of MoS2-based visible detectors and GaN-based UV detectors was demonstrated using common semiconductor fabrication technologies such as photolithography, argon plasma etching, and metal deposition. High performance of a 280 nm and 405 nm dual-wavelength photodetector was realized.The responsivity of the UV detector reached 172.12 A/W, while that of the visible detector reached 17.5 A/W.Meanwhile, both photodetectors achieved high photocurrent gain, high external quantum efficiency, high normalized detection rate, and low noise equivalent power. Our study extends the future application of dual-wavelength detectors for image sensing and optical communication.

Analyses of SELEX-derived ZAP-binding RNA aptamers suggest that the binding specificity is determined by both structure and sequence of the RNA

The zinc-finger antiviral protein(ZAP)is a host factor that specifically inhibits the replication of certain viruses,including murine leukemia virus,Sindbis virus and Ebola virus,by targeting the viral mRNAs for degradation.ZAP directly binds to the target viral mRNA and recruits the cellular RNA degradation machinery to degrade the RNA.No significant sequence similarity or obvious common motifs have been found in the so far identified target viral mRNAs.The minimum length of the target sequence is about 500 nt long.Short workable ZAP-binding RNAs should facilitate further studies on the ZAP-RNA interaction and characterization of such RNAs may provide some insights into the underlying mechanism.In this study,we used the SELEX method to isolate ZAP-binding RNA aptamers.After 21 rounds of selection,ZAP-binding aptamers were isolated.Sequence analysis revealed that they are G-rich RNAs with predicted stem-loop structures containing conserved“GGGUGG”and“GAGGG”motifs in the loop region.Insertion of the aptamer sequence into a luciferase reporter failed to render the reporter sensitive to ZAP.However,overexpression of the aptamers modestly but significantly reduced ZAP’s antiviral activity.Substitution of the conserved motifs of the aptamers significantly impaired their ZAP-binding ability and ZAP-antagonizing activity,suggesting that the RNA sequence is important for specific interaction between ZAP and the target RNA.The aptamers identified in this report should provide useful tools to further investigate the details of the interaction between ZAP and the target RNAs.

Genetic mechanism of the granite buried-hill reservoir of the Penglai 9-1 oilfield in Bohai Sea

The Penglai 9-1 oilfield is the largest granite buried-hill oilfield in China presently,genesis and evolution of the granite buried-hill reservoir is complex.Based on geochemical,geophysical,experimental simulation and other methods,and combined with field geological observation,genetic mechanism of the granite buried-hill reservoir of the Penglai 9-1 oilfield and its hydrocarbon accumulation mode were well investigated.Results showed that the granite was formed by magmatic intrusion along deep faults under intraplate breakup of North China Plate,it was the product of magmatic activities of Yanshan tectonic episode of Circum-Pacific Tectonic Region,and the intrusion time was 160e170 Ma of the Jurassic.Formation of the granite buried-hill reservoir was mainly controlled by the epigenic karstification and tectonic reconstruction,the Yanshanian weathering and denudation controlled macroscopic development characteristics of the granite buried-hill reservoir,and faults and joints formed by Cenozoic tectonic movement promoted modification of the granite buried-hill reservoir.Laterally,thickness of the granite buried-hill reservoir had a positive correlation with fracture density.Vertically,the granite rocks could be divided into five zones:soil zone,sandy zone,broken zone,fracture zone and base rock zone.The upper-middle part(sandy zone,broken zone,fracture zone)of the granite buried hill was the highquality favorable reservoir zone and the main oil-bearing interval.

All-solid-state pseudocapacitive micro-supercapacitors from laser-treated polymer derivatives

We report a simple method for fabricating all-solid-state micro-supercapacitors, utilizing laser writing technology. Porous graphene films with three-dimensional networks induced by laser from commercial polymer was acted as scaffold for loading MnO2, a typical pseudocapacitive materials. Using gel electrolyte, all-solid-state pseudocapacitive micro-supercapacitors were fabricated. Compare to traditional printing and lithography techniques produced micro-supercapacitors, the as-fabricated devices demonstrate high volumetric capacitances, good stability and low leakage current, indicating a scalable and facile approach for future energy storage devices in portable microelectronics.

Influencing factors and prediction of ambient Peroxyacetyl nitrate concentration in Beijing,China

Peroxyacyl nitrates(PANs) are important secondary pollutants in ground-level atmosphere.Accurate prediction of atmospheric pollutant concentrations is crucial to guide effective precautions for before and during specific pollution events. In this study, four models based on the back-propagation(BP) artificial neural network(ANN) and multiple linear regression(MLR) methods were used to predict the hourly average PAN concentrations at Peking University, Beijing, in 2014. The model inputs were atmospheric pollutant data and meteorological parameters. Model 3 using a BP-ANN based on the original variables achieved the best prediction results among the four models, with a correlation coefficient(R) of 0.7089, mean bias error of -0.0043 ppb, mean absolute error of 0.4836?ppb, root mean squared error of 0.5320?ppb, and Willmott's index of agreement of 0.8214. Based on a comparison of the performance indices of the MLR and BP-ANN models, we concluded that the BP-ANN model was able to capture the highly non-linear relationships between PAN concentration and the conventional atmospheric pollutant and meteorological parameters,providing more accurate results than the traditional MLR models did, with a markedly higher goodness of R. The selected meteorological and atmospheric pollutant parameters described a sufficient amount of PAN variation, and thus provided satisfactory prediction results. More specifically, the BP-ANN model performed very well for capturing the variation pattern when PAN concentrations were low. The findings of this study address some of the existing knowledge gaps in this research field and provide a theoretical basis for future regional air pollution control.

TSUNAMI:Translational Bioinformatics Tool Suite for Network Analysis and Mining

Gene co-expression network(GCN)mining identifies gene modules with highly correlated expression profiles across samples/conditions.It enables researchers to discover latent gene/molecule interactions,identify novel gene functions,and extract molecular features from certain disease/condition groups,thus helping to identify disease bio-markers.However,there lacks an easy-to-use tool package for users to mine GCN modules that are relatively small in size with tightly connected genes that can be convenient for downstream gene set enrichment analysis,as well as modules that may share common members.To address this need,we developed an online GCN mining tool package:TSUNAMI(Tools SUite for Network Analysis and MIning).TSUNAMI incorporates our state-of-the-art lmQCM algorithm to mine GCN modules for both public and user-input data(microarray,RNA-seq,or any other numerical omics data),and then performs downstream gene set enrichment analysis for the identified modules.It has several features and advantages:1)a user-friendly interface and real-time co-expression network mining through a web server;2)direct access and search of NCBI Gene Expression Omnibus(GEO)and The Cancer Genome Atlas(TCGA)databases,as well as user-input gene ex-pression matrices for GCN module mining;3)multiple co-expression analysis tools to choose from,all of which are highly flexible in regards to parameter selection options;4)identified GCN modules are summarized to eigengenes,which are convenient for users to check their correlation with other clinical traits;5)integrated downstream Enrichr enrichment analysis and links to other gene set enrichment tools;and 6)visualization of gene loci by Circos plot in any step of the process.The web service is freely accessible through URL:https://biolearns.medicine.iu.edu/.Source code is available at https://github.com/huangzhii/TSUNAMI/.

Multi-functionalized nanofibers with reactive oxygen species scavenging capability and fibrocartilage inductivity for tendon-bone integration

The presence of excessive reactive oxygen species(ROS)after injuries to the enthesis could lead to cellular oxidative damage,high inflammatory response,chronic inflammation,and limited fibrochondral inductivity,making tissue repair and functional recovery difficult.Here,a multifunctional silk fibroin nanofiber modified with polydopamine and kartogenin was designed and fabricated to not only effectively reduce inflammation by scavenging ROS in the early stage of the enthesis healing but also enhance fibrocartilage formation with fibrochondrogenic induction in the later stages.The in vitro results confirmed the antioxidant capability and the fibrochondral inductivity of the functionalized nanofibers.In vivo studies showed that the multifunctional nanofiber can significantly improve the integration of tendon-bone and accelerate the regeneration of interface tissue,resulting in an excellent biomechanical property.Thus,the incorporation of antioxidant and bio-active molecules into extracellular matrix-like biomaterials in interface tissue engineering provides an integrative approach that facilitates damaged tissue regeneration and functional recovery,thereby improving the clinical outcome of the engineered tissue.