The Developmental Difficulties and Prospects of Chinese-style Animation:Taking Yao-Chinese Folktales as an Example

As one of the important means to spread Chinese traditional culture,Chinese-style animation has been on the rise in recent years.Many excellent works have emerged,among which the Chinese animated short film series Yao-Chinese Folktales is one of the good representatives.It aroused a strong reaction from the audience as soon as it was released on the Internet,and gave the audience a shock to their mind and vision,which has brought some thoughts to the development of Chinese-style animation.Following a linear logic chain of past-present-future Chinese-style animation’s development,this paper takes Yao-Chinese Folktales as an example and analyzes the developmental difficulties and prospects of Chinese-style animation.At the end of the paper,some suggestions about the development path of Chinese-style animation’s future are given from the aspects of creation and dissemination,for the aim of promoting the Chinese-style animation’s development and spreading the Chinese traditional culture.

基于Copula方法的城市排水排涝设计标准协调性研究

我国排水系统和排涝系统分属不同部门管理,遵循不同设计规范,缺乏统一的标准衔接方法。为解决排水排涝系统设计标准不衔接造成的潜在内涝风险和投资浪费问题,研究以中山和珠海市为研究对象,基于Copula方法和最可能设计组合构建了排水排涝设计标准的衔接方法,计算了不同Kendall重现期条件下的最可能排水排涝设计降水量组合。结果表明,Copula理论可有效构建不同时段长降水序列的联合分布,Copula理论分布与经验分布之间拟合良好;以中山市主城区为例,排水排涝系统最可能设计降雨量组合结果表明,原排水系统设计规模偏小,原排涝系统设计规模偏大,据此可针对原规划方案进行科学调整。

鹅源鼠伤寒沙门菌的分离与耐药性分析

为开展鹅群鼠伤寒沙门菌感染的预防和控制,本试验对临床病料进行细菌分离,继而对分离菌株进行生化试验、沙门菌属特异性invA基因PCR扩增和血清型鉴定,药敏试验和β-内酰胺酶类耐药基因CTX-M-9的检测。结果显示,本试验共分离到10株鹅源鼠伤寒沙门菌分离株,5株为多重耐药菌株,其中3株对阿莫西林、美洛西林耐药,2株含有β-内酰胺酶类耐药基因CTX-M-9。

油酸人工抗原的合成及其单克隆抗体的制备

为建立奶牛血清中油酸(OA)含量的免疫学检测方法,将油酸用活泼酯法分别与牛血清白蛋白(BSA)和卵清白蛋白(OVA)进行偶联,制备人工抗原和包被原;利用薄层色谱法(TLC)和红外光谱法鉴定人工抗原合成是否成功;用OA-BSA常规免疫6周龄雌性BALB/c小鼠,取小鼠脾细胞和骨髓瘤细胞进行融合,用间接ELISA筛选出阳性细胞株,再用有限稀释法进行亚克隆;采用小鼠体内诱生法制备腹水,Hi Trap Protein G HP纯化柱纯化腹水,并对纯化后的腹水用ELISA检测抗体效价,以及抗体亚型鉴定。结果表明,TLC监测抗原合成过程中有新物质生成,红外图谱显示OA-BSA波谱形态与BSA相似且拥有OA的特征峰;小鼠血清抗体效价为1∶12 800,用ELISA方法筛选出1株细胞G-1,抗体亚型为Ig G1型。为建立油酸的免疫学检测方法奠定了基础。