This study is to report a ZnSe quantum dot with a large two-photon absorption cross section and good biocompatibility,which can be used in bioimaging.Fluorescence emission at 410 nm is observed in the quantum dot unde...This study is to report a ZnSe quantum dot with a large two-photon absorption cross section and good biocompatibility,which can be used in bioimaging.Fluorescence emission at 410 nm is observed in the quantum dot under 760-nm laser excitation.These biocompatible quantum dots exhibit a two-photon cross-section of 9.1×105 GM(1 GM=10-50 cm4·s/photon).Two-photon excited laser scanning microscopic images show that cells co-cultured with ZnSe quantum dots are found in the blue channel at a fluorescence intensity that is 14.5 times that of control cells not cocultured with quantum dots.After incubating zebrafish larvae with ZnSe quantum dots for 24 h,the fluorescence intensity of the yolk sac stimulated by ultraviolet light is 2.9 times that of the control group.The proposed material shows a great potential application in biological imaging.展开更多
Combing the time corelated single photon counting(TCSPC)with fuorescence lifetime imaging microscopy(FLIM)provides promising opportunities in revealing important information on the microenvironment of cells and tissue...Combing the time corelated single photon counting(TCSPC)with fuorescence lifetime imaging microscopy(FLIM)provides promising opportunities in revealing important information on the microenvironment of cells and tissues,but the applications are thus far mainly limited by the accuracy and precision of the TCSPC-FLIM technique.Here we present a comprehensive in-vestigation on the perforance of two data analysis methods,the first moment(M_(1))method and the conventional least squares(Fitting)method,in quantifying fuorescence lifetime.We found that the Mp method is more superior than the Fitting method when the lifetime is short(70-400ps)or the signal intensity is weak(<10^(3) photons).展开更多
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61774062 and U20A20206)the Science and Techology Program of Guangzhou City,China(Grant No.2019050001)the Natural Science Foundation of Guangdong Province,China(Grant Nos.2018A030313854 and2016A030308010)。
文摘This study is to report a ZnSe quantum dot with a large two-photon absorption cross section and good biocompatibility,which can be used in bioimaging.Fluorescence emission at 410 nm is observed in the quantum dot under 760-nm laser excitation.These biocompatible quantum dots exhibit a two-photon cross-section of 9.1×105 GM(1 GM=10-50 cm4·s/photon).Two-photon excited laser scanning microscopic images show that cells co-cultured with ZnSe quantum dots are found in the blue channel at a fluorescence intensity that is 14.5 times that of control cells not cocultured with quantum dots.After incubating zebrafish larvae with ZnSe quantum dots for 24 h,the fluorescence intensity of the yolk sac stimulated by ultraviolet light is 2.9 times that of the control group.The proposed material shows a great potential application in biological imaging.
基金supported by National Basic Research Program of China(Grant No.2011CB910401)the Science Fund for Creative Research Group of China(Grant No.61121004)+1 种基金the National Natural Sci-ence Foundation of China(Grant Nos.30970691 and 61275059)the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry of China,and the Program for New Century Excellent Talents in University of China(Grant No.NCET-10-0407).
文摘Combing the time corelated single photon counting(TCSPC)with fuorescence lifetime imaging microscopy(FLIM)provides promising opportunities in revealing important information on the microenvironment of cells and tissues,but the applications are thus far mainly limited by the accuracy and precision of the TCSPC-FLIM technique.Here we present a comprehensive in-vestigation on the perforance of two data analysis methods,the first moment(M_(1))method and the conventional least squares(Fitting)method,in quantifying fuorescence lifetime.We found that the Mp method is more superior than the Fitting method when the lifetime is short(70-400ps)or the signal intensity is weak(<10^(3) photons).
