期刊文献+
共找到3篇文章
< 1 >
每页显示 20 50 100
非天然氨基酸定点偶联抗人类表皮生长因子受体2-抗体偶联药物的药理学活性 被引量:4
1
作者 梁学军 宫丽颖 +2 位作者 周菲 周德敏 祝静静 《北京大学学报(医学版)》 CAS CSCD 北大核心 2019年第5期797-804,共8页
目的:考察用非天然氨基酸定点偶联技术获得的均一性良好的抗人类表皮生长因子受体2(human epidermal growth factor receptor 2,HER2)-抗体偶联药物(antibody drug conjugate,ADC),对9种不同HER2表达量的肿瘤细胞的增殖抑制活性,以及对... 目的:考察用非天然氨基酸定点偶联技术获得的均一性良好的抗人类表皮生长因子受体2(human epidermal growth factor receptor 2,HER2)-抗体偶联药物(antibody drug conjugate,ADC),对9种不同HER2表达量的肿瘤细胞的增殖抑制活性,以及对5种异体移植的肿瘤小鼠模型的肿瘤生长抑制效果。方法:用QIFI试剂盒通过流式细胞仪检测HER2在BT-474、Calu-3、MCF-7、MDA-MB-231、MDA-MB-468、SK-BR-3、SK-OV-3、HCC1954、NCI-N87共9种肿瘤细胞中的表达。对9种肿瘤细胞进行培养,铺板过夜后分别加入梯度稀释的抗HER2-ADC、曲妥珠单抗-美坦新偶联物、AS269、pAF-AS269、紫杉醇5种药物,然后培养72 h或96 h,检测这5种药物对肿瘤细胞的增殖抑制活性。选择HER2阳性肿瘤细胞HCC1954、BT-474、SK-OV-3、NCI-N87和HER2阴性肿瘤细胞MDA-MB-468,分别接种到5~6周龄的BALB/c裸小鼠身上,待肿瘤长到一定体积后,分别注射抗HER2-ADC、曲妥珠单抗-美坦新偶联物、曲妥珠单抗、紫杉醇4种药物和空白对照磷酸盐缓冲液,考察药物的抑瘤效果。结果:流式细胞仪检测结果显示,SK-OV-3、NCI-N87、SK-BR-3、Calu-3、HCC1954、BT-474这6株肿瘤细胞的HER2表达量较高,每个细胞表面HER2受体数在43~80万个,比另外3株MDA-MB-231、MCF-7、MDA-MB-468肿瘤细胞的HER2表达水平高50倍以上。药物对9种肿瘤细胞的增殖抑制活性结果显示,抗HER2-ADC对HER2高表达的细胞有很强的抑制细胞生长活性,对SK-OV-3、NCI-N87、SK-BR-3、Calu-3、HCC1954、BT-474的半数抑制浓度分别为46、17、17、161、125、50 pmol/L。在动物体内药效试验中,抗HER2-ADC在所有HER2阳性表达的肿瘤模型中都表现了较强的呈剂量依赖的抗肿瘤活性,在NCI-N87异种移植肿瘤模型中,与曲妥珠单抗及曲妥珠单抗-美坦新偶联物相比,相同剂量的抗HER2-ADC表现出更好的抗肿瘤活性,其相对肿瘤增殖率约为二者的1/30至1/20,在HCC1954模型中表现出肿瘤的完全消退和治愈效果;抗HER2-ADC对HER2低表达MDA-MB-468肿瘤细胞移植瘤模型没有效果。与曲妥珠单抗-美坦新偶联物相比,抗HER2-ADC表现出相同或更好的抗肿瘤活性。结论:用非天然氨基酸定点偶联技术获得的抗HER2-ADC在细胞体外和动物体内实验中对HER2高表达的肿瘤具有明显的抑制效果。 展开更多
关键词 抗体偶联药物 人类表皮生长因子受体2 非天然氨基酸
下载PDF
GIS SUPPORTED HEDONIC MODEL FOR ASSESSING PROPERTY VALUE IN WEST OAKLAND, CALIFORNIA 被引量:1
2
作者 zhou de-min XU Jian-chun GONG Hui-li 《Chinese Geographical Science》 SCIE CSCD 2006年第1期70-78,共9页
A hedonic linear regression model is constructed in this paper to estimate property value. In our model, the property value (sales price) is a function of several selected variables such as the property characteristic... A hedonic linear regression model is constructed in this paper to estimate property value. In our model, the property value (sales price) is a function of several selected variables such as the property characteristics, social neighborhoods, level of neighborhood environmental contaminations, level of neighborhood crimes, and locational accessibility to jobs or services. Definitions and calculation of these variables are approached by using Geographic Information System tools. For improving estimation, gravity model is employed to measure both levels of neighborhood toxic sites and crimes; and a time-based method is used to measure the locational accessibility rather than simple straight-line distance measurement. This study discovers that the relationship between house value and its nearby highway is nonlinear. The methodology could help policy makers assess the external effects of a property. Our model also could be used potentially to identify the current and historic trends of development caused by neighborhood or environments change in the study area. 展开更多
关键词 GIS 地理信息系统 奥克兰 加利福尼亚 邻域 可达性
下载PDF
A model supported by GIS for locating and quantifying PM_(2.5) emission originated from residential wood burning 被引量:1
3
作者 zhou de-min RADKE John +2 位作者 TIAN Yong-qian XU Jian-chun MU Lan 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2005年第5期861-865,共5页
A research method was presented for spatially quantifying and allocating the potential activity of a fine particle matter emission ( PM2.5 ), which originated from residential wood burning (RWB) in this study. Dem... A research method was presented for spatially quantifying and allocating the potential activity of a fine particle matter emission ( PM2.5 ), which originated from residential wood burning (RWB) in this study. Demographic, hypsographic, climatic and topographic data were compiled and processed within a geographic information system(GIS), and as independent variables put into a linear regression model for describing spatial distribution of the potential activity of residential wood burning as primary heating source. In order to improve the estimation, the classifications of urban, suburban and rural were redefined to meet the specifications of this application. Also, several definitions of forest accessibility were tested for estimation. The results suggested that the potential activity of RWB was mostly determined by elevation of a location, forest accessibility, urban/non-urban position, climatic conditions and several demographic variables. The linear regression model could explain approximately 86% of the variation of surveyed potential activity of RWB. The analysis results were validated by employing survey data collected mainly from a WebGIS based phone interview over the study area in central California. Based on lots free public GIS data, the model provided an easy and ideal tool for geographic researchers, environmental planners and administrators to understand where and how much PM2.5 emission from RWB was contributed to air quality. With this knowledge they could identify regions of concern, and better plan mitigation strategies to improve air quality. Furthermore, it allows for future adjustment on some parameters as the spatial analysis method is implemented in the different regions or various eco-social models. 展开更多
关键词 residential wood burning(RWB) PM2.5 demographical characteristies Geographic information system(GIS) stepwise linear regression
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部