塞来昔布对新生大鼠高氧致肺损伤的干预作用

目的探讨塞来昔布对高氧致新生大鼠肺损伤的影响,并分析可能的作用机制。方法以持续暴露在高浓度氧中的方法建立新生大鼠肺损伤模型。将30只新生大鼠随机分为对照组(n=10)、模型组(n=10)、实验组(n=10)。实验组于模型建立成功后腹腔注射1.5 mg·kg^(-1)塞来昔布(连续7 d)。对照组、模型组于相同时间点腹腔注射等量0.9%NaCl。以苏木精-伊红(HE)染色法观察大鼠肺组织病理学变化;以酶联免疫吸附法(ELISA)测定各组肺组织中活性氧簇(ROS)水平;以蛋白质印迹法测定各组大鼠肺组织中核因子-κB/诱导型一氧化氮合酶(NF-κB/iNOS)信号通路相关蛋白表达水平。结果对照组肺组织肺泡结构完整,肺泡壁未增厚,未见充血及炎性细胞浸润;模型组肺泡间隙增加且充血水肿,肺泡腔可见炎性渗出液,肺组织结构紊乱;与模型组比较,实验组病理变化明显减轻。对照组、模型组和实验组大鼠的肺组织ROS浓度分别为(285.79±35.55)、(456.77±63.23)和(389.87±53.77)U·mL^(-1);NF-κB p65蛋白表达水平分别为0.19±0.03、1.06±0.16和0.89±0.13;iNOS蛋白表达水平分别为0.33±0.06、1.23±0.18和0.96±0.13。模型组、实验组上述指标与对照组比较,差异均有统计学意义(P<0.01或P<0.05);实验组的上述指标与模型组比较,差异均有统计学意义(P<0.01或P<0.05)。结论塞来昔布对高氧致新生大鼠肺损伤具有保护作用,可能是通过抑制氧化应激,进而抑制NF-κB/iNOS信号通路活化实现的。

A Novel Approach to Intra-operative Planning for Prostate Brachytherapy

This paper presents an efficient and stable algorithm to intra-operative planning for prostate brachytherapy. As a heuristic approach, the algorithm selects one seed at a step and iterates until predefined prostate volume is covered by prescribed dose. For each step, potential seeds are evaluated according to the ability to irradiate the target while sparing adjacent organs at risk. And its influence on dose homogeneity is also taken into account. Furthermore, a flexible mechanism is adopted to limit the number of used needles. The mechanism selects acceptable sub-optimal seed in existing needles instead of optimal seed that requires adding a new needle. The efficacy of algorithm is evaluated on five clinical cases. Compared with state-of-the-art methods,this algorithm is efficient and stable without the trial and error process of determining various parameters. These advantages make the algorithm suitable for intra-operative real-time treatment planning.