The stem and branch extract of Tripterygium wilfordii(Celastraceae)afforded seven new dihydroagarofuran sesquiterpene polyesters[tripterysines A‒G(1‒7)]and eight known ones(8‒15).The chemical structures of these new c...The stem and branch extract of Tripterygium wilfordii(Celastraceae)afforded seven new dihydroagarofuran sesquiterpene polyesters[tripterysines A‒G(1‒7)]and eight known ones(8‒15).The chemical structures of these new compounds were established based on combinational analysis of HR-ESI-MS and NMR techniques.The absolute configurations of tripterysines A‒C(1‒3)and E‒G(5‒7)were determined by X-ray crystallographic analysis and circular dichroism spectra.All the compounds were screened for their inhibitory effect on inflammation through determining their inhibitory effect on nitric oxide production in LPS-induced RAW 264.7 cells and the secretion of inflammatory cytokines TNF-αand IL-6 in LPS-induced BV2 macrophages.Compound 9 exhibited significant inhibitory activity on NO production with an IC50 value of 8.77μmol·L−1.Moreover,compound 7 showed the strongest inhibitory effect with the secretion of IL-6 at 27.36%.展开更多
基金the Natural Science Foundation Committee of Guangdong Province(No.32220053)the National Key R&D Program of China(No.2017YFC1703800).
文摘The stem and branch extract of Tripterygium wilfordii(Celastraceae)afforded seven new dihydroagarofuran sesquiterpene polyesters[tripterysines A‒G(1‒7)]and eight known ones(8‒15).The chemical structures of these new compounds were established based on combinational analysis of HR-ESI-MS and NMR techniques.The absolute configurations of tripterysines A‒C(1‒3)and E‒G(5‒7)were determined by X-ray crystallographic analysis and circular dichroism spectra.All the compounds were screened for their inhibitory effect on inflammation through determining their inhibitory effect on nitric oxide production in LPS-induced RAW 264.7 cells and the secretion of inflammatory cytokines TNF-αand IL-6 in LPS-induced BV2 macrophages.Compound 9 exhibited significant inhibitory activity on NO production with an IC50 value of 8.77μmol·L−1.Moreover,compound 7 showed the strongest inhibitory effect with the secretion of IL-6 at 27.36%.
