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仔猪先天性震颤瘟病毒RT-PCR检测方法的建立 被引量:1
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作者 吴松松 周荷田 +3 位作者 张文波 邓舜洲 张志庆 杨丹凤 《动物医学进展》 北大核心 2019年第4期28-31,共4页
旨在建立检测仔猪先天性震颤瘟病毒(APPV)的逆转录-聚合酶链反应(RT-PCR)方法。根据GenBank上登录的APPV基因组序列,设计1对引物,以临床上表现典型震颤仔猪的淋巴结为材料,提取RNA,建立了RT-PCR方法,并进行敏感性、重复性和特异性试验,... 旨在建立检测仔猪先天性震颤瘟病毒(APPV)的逆转录-聚合酶链反应(RT-PCR)方法。根据GenBank上登录的APPV基因组序列,设计1对引物,以临床上表现典型震颤仔猪的淋巴结为材料,提取RNA,建立了RT-PCR方法,并进行敏感性、重复性和特异性试验,对其扩增产物测序比对分析;利用该方法,对临床病料进行检测。结果显示,从典型震颤仔猪的淋巴结中均扩增到与预期大小有一致的片段,经测序比对,扩增片段序列与APPV相应片段的核苷酸同源性在89%以上;该方法只能扩增出APPV片段,而其他几种常见猪病毒均为阴性,3次重复检测结果基本一致,可检测最低cDNA浓度为184.11ng/μL;对临床病料检测,典型病例的检出率达100%。试验结果表明,建立的RT-PCR方法具有良好的特异性、重复性、敏感性等优点,可用于临床APPV引起的仔猪先天性震颤早期检测、病毒鉴定和分子流行病学调查。 展开更多
关键词 仔猪先天性震颤 瘟病毒 逆转录-聚合酶链反应
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Laser Induced Light-Force Interaction in the Optical Near-Field Region
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作者 ZHU Xing LING Yong +4 位作者 HUANG Gui-song zhou he-tian DAI Yuan-dong WU Ke GAN Zi-zhao 《Chinese Physics Letters》 SCIE CAS CSCD 1998年第3期165-167,共3页
Laser light can induce many effects on the vicinity of the sample surface due to the proximity effects,such as the field enhancement at the surface of a small conducting particle;plasma exchange effect between the met... Laser light can induce many effects on the vicinity of the sample surface due to the proximity effects,such as the field enhancement at the surface of a small conducting particle;plasma exchange effect between the metallic tip and substrate surface.We have observed a new optical tip-sample interaction:laser induced force as the results of light pressure in the near-field region.Under the side illumination of a laser light,an additional attractive force was observed between a dielectric tip and various samples of C60,optical glass,YBa_(2)Cu_(3)O_(7-d)(YBCO)thin film.No such effect was observed in metallic samples.This optical binding force is due to the multiple scattering of light between two close objects.The results are in good agreement with the recent theoretical calculation.The effects of the optical refraction index,thermal expansion of probe tip due to laser radiation are discussed. 展开更多
关键词 SCATTERING METALLIC effect
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