乙型肝炎是一种常见的感染性疾病,乙型肝炎表面抗原(hepatitis B surface antigen,HBsAg)是乙型肝炎病毒(hepatitis B virus,HBV)感染的重要血清标志物之一。慢性HBV感染者血清持续低水平HBsAg表达是在多种因素的综合作用下,HBV自身复...乙型肝炎是一种常见的感染性疾病,乙型肝炎表面抗原(hepatitis B surface antigen,HBsAg)是乙型肝炎病毒(hepatitis B virus,HBV)感染的重要血清标志物之一。慢性HBV感染者血清持续低水平HBsAg表达是在多种因素的综合作用下,HBV自身复制与宿主免疫清除达到一种相对稳定而复杂的动态平衡状态,其HBV DNA呈现低复制,因此,HBV感染者血清持续低水平HBsAg状态下仍具有潜在传染风险性,已受到了传染病学、流行病学等领域专家的广泛关注。目前,有关血清持续低水平HBsAg表达的形成机制尚不完全明确,但持续低水平HBsAg表达形成机制中存在着宿主免疫耐受现象被广大学者所认可,然而如何打破宿主对持续低水平HBsAg表达的免疫耐受,实现宿主对病毒的清除是现阶段面临的严峻挑战,为此该文对慢性HBV感染者血清持续低水平HBsAg表达免疫耐受机制的相关研究进展进行综述,为打破免疫耐受、有效清除低水平HBV提供新思路。展开更多
Objective: To evaluate the effect of Buyang Huanwu Decoction(补阳还五汤,BYHWD) on glial scar after intracerebral hemorrhage(ICH) and investigate the underlying mechanism.Methods: Collagenase type Ⅶ(0.5 U) was injecte...Objective: To evaluate the effect of Buyang Huanwu Decoction(补阳还五汤,BYHWD) on glial scar after intracerebral hemorrhage(ICH) and investigate the underlying mechanism.Methods: Collagenase type Ⅶ(0.5 U) was injected stereotaxically into right globus pallidus to induce ICH model.One hundred and twenty SpragueDawley rats were randomly divided into 3 groups according to a random number table,including normal group(n=40),ICH model group(n=40) and BYHWD group(n=40),respectively.After ICH,the rats in the BYHWD group were intragastrically administered with BYHWD(4.36 g/kg) once a day for 21 days,while the rats in ICH group were administered with equal volume of distilled water for 21 days,respectively.Double immunolabeling was performed for proliferating cell nuclear antigen(PCNA)+/glial ?brillary acidic protein(GFAP)+ nuclei.The expression of GFAP and leukemia inhibitory factor(LIF) was evaluated by immunohistochemistry and quantitative real-time reverse transcription-polymerase chain reaction(RT-PCR).Results: The astrocytes with hypertrophied morphology around the hematoma was observed on day 3 after ICH.The number of GFAP positive cells and GFAP m RNA levels increased notably on day 3 and reached the peak on day 14 post-ICH(P<0.01).PCNA+/GFAP+ nuclei were observed around the hematoma and reached the peak on day 14 post-ICH(P<0.01).In addition,LIF-positive astrocytes and LIF m RNA level in the hemorrhagic region increased signi?cantly till day 14 post-ICH(P<0.01).However,BYHWD not only reduced the number of PCNA+/GFAP+ nuclei,but also decreased GFAP and LIF levels(P<0.05).Conclusion:BYHWD could attenuate ICH-induced glial scar by downregulating the expression of LIF in the rats.展开更多
文摘乙型肝炎是一种常见的感染性疾病,乙型肝炎表面抗原(hepatitis B surface antigen,HBsAg)是乙型肝炎病毒(hepatitis B virus,HBV)感染的重要血清标志物之一。慢性HBV感染者血清持续低水平HBsAg表达是在多种因素的综合作用下,HBV自身复制与宿主免疫清除达到一种相对稳定而复杂的动态平衡状态,其HBV DNA呈现低复制,因此,HBV感染者血清持续低水平HBsAg状态下仍具有潜在传染风险性,已受到了传染病学、流行病学等领域专家的广泛关注。目前,有关血清持续低水平HBsAg表达的形成机制尚不完全明确,但持续低水平HBsAg表达形成机制中存在着宿主免疫耐受现象被广大学者所认可,然而如何打破宿主对持续低水平HBsAg表达的免疫耐受,实现宿主对病毒的清除是现阶段面临的严峻挑战,为此该文对慢性HBV感染者血清持续低水平HBsAg表达免疫耐受机制的相关研究进展进行综述,为打破免疫耐受、有效清除低水平HBV提供新思路。
基金the National Natural Science Foundation of China(No.81202625,30400581,30873221 and 81173175)the Project for New Century Excellent Talents(NCET-11-0522)+2 种基金the Hunan Provincial Natural Science Foundation(No.07JJ5007 and 10JJ2023)the Hubei Provincial Natural Science Foundation(No.2017CFB468)the Key Laboratory of Cardiovascular and Cerebrovascular Diseases Translational Medicine(Three Gorges University,No.2016KXN06)
文摘Objective: To evaluate the effect of Buyang Huanwu Decoction(补阳还五汤,BYHWD) on glial scar after intracerebral hemorrhage(ICH) and investigate the underlying mechanism.Methods: Collagenase type Ⅶ(0.5 U) was injected stereotaxically into right globus pallidus to induce ICH model.One hundred and twenty SpragueDawley rats were randomly divided into 3 groups according to a random number table,including normal group(n=40),ICH model group(n=40) and BYHWD group(n=40),respectively.After ICH,the rats in the BYHWD group were intragastrically administered with BYHWD(4.36 g/kg) once a day for 21 days,while the rats in ICH group were administered with equal volume of distilled water for 21 days,respectively.Double immunolabeling was performed for proliferating cell nuclear antigen(PCNA)+/glial ?brillary acidic protein(GFAP)+ nuclei.The expression of GFAP and leukemia inhibitory factor(LIF) was evaluated by immunohistochemistry and quantitative real-time reverse transcription-polymerase chain reaction(RT-PCR).Results: The astrocytes with hypertrophied morphology around the hematoma was observed on day 3 after ICH.The number of GFAP positive cells and GFAP m RNA levels increased notably on day 3 and reached the peak on day 14 post-ICH(P<0.01).PCNA+/GFAP+ nuclei were observed around the hematoma and reached the peak on day 14 post-ICH(P<0.01).In addition,LIF-positive astrocytes and LIF m RNA level in the hemorrhagic region increased signi?cantly till day 14 post-ICH(P<0.01).However,BYHWD not only reduced the number of PCNA+/GFAP+ nuclei,but also decreased GFAP and LIF levels(P<0.05).Conclusion:BYHWD could attenuate ICH-induced glial scar by downregulating the expression of LIF in the rats.
