To broaden the genetic basis and overcome the yield plateau in Asian cultivated rice, the exploitation and utilization of favorable alleles from rice species with the AA genome has become important and urgent in moder...To broaden the genetic basis and overcome the yield plateau in Asian cultivated rice, the exploitation and utilization of favorable alleles from rice species with the AA genome has become important and urgent in modern breeding programs. Four different interspecific populations were used to detect quantitative trait locus (QTL) for seed length, including a BC4F2 population derived from Oryza glumaepatula crossed with Dianjingyou 1 (a japonica cultivar), a BC4F2 population derived from O. nivara crossed with Dianjingyou 1, a BCzF1 population derived from a cross between O. Iongistaminata and RD23 (an indica cultivar), and a BCsF1 population derived from a cross between O. glaberrima and Dianjingyou 1. The QTLs for seed length in four different populations were termed as SL-3a, SL-3b, SL-3c and SL-3d, respectively. They had good collinearity and accounted for 49% to 60% of the phenotypic variations. Sequencing data indicated that four QTLs were different alleles of GS3 which were responsible for the seed length variation between O. sativa and its four AA genome relatives. These results will be valuable for confirming the evolution of GS3 and also be helpful for rice breeding.展开更多
Hybrid sterility between Oryza glaberrima and O. sativa seriously hampers the introgression of favorable genes from each other. In order to further understand this issue, identification and isolation of hybrid sterili...Hybrid sterility between Oryza glaberrima and O. sativa seriously hampers the introgression of favorable genes from each other. In order to further understand this issue, identification and isolation of hybrid sterility QTLs as single Mendelian factors are an effective strategy. A genetic map was constructed using a BC1 F1 population derived from a cross between an O. safiva japonica cultivar and an O. glaberrima accession. Four main-effect QTLs for pollen sterility were detected in the BC1F1. Five BC8F1 advanced backcross populations were developed via successive backcrosses based on phenotype and molecular selections. The BC8F1 populations showed bimodal distribution for pollen fertility and could be classified into semi-sterile and fertile types, fitting single Mendilian factor inheritance ratios. Three QTLs detected in the BC1F1 corresponding to qSS-3, qSS-6a and qSS-7 were mapped on chromosomes 6, 3 and 7, respectively, as single Mendilian factors.展开更多
基金funded by grants from the Ministry of Agriculture, China (Grant No. 2009ZX08009-107B)National Natural Science Foundation of China (Grant Nos. 31201196, 31160275, U1036605 and 31000704)+1 种基金Yunnan Provincial National Science Foundation, China (Grant Nos. 2011FB118 and 2010CI035)Institute of Food and Crop Research, Yunnan Academy of Agricultural Sciences, China (Grant No. 2013LZS001)
文摘To broaden the genetic basis and overcome the yield plateau in Asian cultivated rice, the exploitation and utilization of favorable alleles from rice species with the AA genome has become important and urgent in modern breeding programs. Four different interspecific populations were used to detect quantitative trait locus (QTL) for seed length, including a BC4F2 population derived from Oryza glumaepatula crossed with Dianjingyou 1 (a japonica cultivar), a BC4F2 population derived from O. nivara crossed with Dianjingyou 1, a BCzF1 population derived from a cross between O. Iongistaminata and RD23 (an indica cultivar), and a BCsF1 population derived from a cross between O. glaberrima and Dianjingyou 1. The QTLs for seed length in four different populations were termed as SL-3a, SL-3b, SL-3c and SL-3d, respectively. They had good collinearity and accounted for 49% to 60% of the phenotypic variations. Sequencing data indicated that four QTLs were different alleles of GS3 which were responsible for the seed length variation between O. sativa and its four AA genome relatives. These results will be valuable for confirming the evolution of GS3 and also be helpful for rice breeding.
基金funded partially by the Ministry of Science and Technology Foundation of China(Grant No.2006CB708207)the Ministry of Agriculture,China(Grant No.2009ZX08009-107B)
文摘Hybrid sterility between Oryza glaberrima and O. sativa seriously hampers the introgression of favorable genes from each other. In order to further understand this issue, identification and isolation of hybrid sterility QTLs as single Mendelian factors are an effective strategy. A genetic map was constructed using a BC1 F1 population derived from a cross between an O. safiva japonica cultivar and an O. glaberrima accession. Four main-effect QTLs for pollen sterility were detected in the BC1F1. Five BC8F1 advanced backcross populations were developed via successive backcrosses based on phenotype and molecular selections. The BC8F1 populations showed bimodal distribution for pollen fertility and could be classified into semi-sterile and fertile types, fitting single Mendilian factor inheritance ratios. Three QTLs detected in the BC1F1 corresponding to qSS-3, qSS-6a and qSS-7 were mapped on chromosomes 6, 3 and 7, respectively, as single Mendilian factors.