Fruit firmness is an important quality trait of apple fruit texture,and the pre-harvest ripening period is the key period for the formation of apple fruit texture.Expansin is a cell wall loosing protein family that ha...Fruit firmness is an important quality trait of apple fruit texture,and the pre-harvest ripening period is the key period for the formation of apple fruit texture.Expansin is a cell wall loosing protein family that has four subfamilies:α-expansin(EXPA),β-expansin(EXPB),expansin-like A(EXLA),and expansin-like B(EXLB).In this study,we investigated the key period of pre-harvest texture formation in‘Golden Delicious’apples based on fruit longitudinal diameter,transverse diameter,firmness,tissue structure,respiration intensity,ethylene release rate,and expansin activity.Within the 10 days before harvest,the fruit was found to reach maturity.Semi-quantitative RT-PCR revealed that most of the expansins were expressed at the ripening stage before harvest.The biological function of the EXLB subfamily gene,MdEXLB1,was further identified,and its subcellular localization on the cell wall was confirmed by transient transformation experiments.Compared with the wild type(WT),the transgenic tomato lines overexpressing MdEXLB1 had lower plant height,earlier fruiting period,fewer days for fruit ripening,higher fruit maturity,lower fruit firmness,higher fruit expansin activity,more discrete flesh cell structure,and accelerated fruit ripening process.Overall,this is the first study to propose that the apple EXLB subfamily gene,MdEXLB1,has biological functions and plays an important role in promoting fruit ripening and softening.展开更多
Based on 2012 input-output table of Shandong province and the official statistics, this article uses the input-output analysis method to build the input-output table. On the basis of the development of service industr...Based on 2012 input-output table of Shandong province and the official statistics, this article uses the input-output analysis method to build the input-output table. On the basis of the development of service industry in Shandong province's ability, we make a theoretical analysis framework of a comprehensive in-depth analysis on pull employment, creatively will cost tax rate, fully and completely labor capital and the combination of both the industry development space. So that the industry's ability to pull the employment can be considered, and to realize rapid and sustained development of service industry in Shandong province.展开更多
This paper puts forward the connotation of enterprise green management and green management performance evaluation,analyzes the characteristics of enterprise green management performance evaluation and decomposes its ...This paper puts forward the connotation of enterprise green management and green management performance evaluation,analyzes the characteristics of enterprise green management performance evaluation and decomposes its content.Using the triple performance method,the paper constructs the performance evaluation index system of enterprise green management.Taking BH Petrochemical Co.,Ltd.at the high-efficiency ecological economic zone of the Yellow River Delta as an example,by using analytic hierarchy process(AHP)and Matlab programming,this paper determines the weight of each index in the enterprise green management performance evaluation index system,the fuzzy comprehensive evaluation method is used to evaluate the green management performance evaluation of industrial enterprises.The results show that the performance evaluation index system of enterprise green management constructed by"triple performance method"has important theoretical significance and application value for evaluating and guiding enterprises to strengthen their green management.展开更多
基金the National Key R&D Program of China(2016YFD0201100)the earmarked fund for China Agriculture Research System(CARS-27)+1 种基金the National Natural Science Foundation of China(31672116)the Natural Science Foundation of Hebei Province,China(C2021204134).
文摘Fruit firmness is an important quality trait of apple fruit texture,and the pre-harvest ripening period is the key period for the formation of apple fruit texture.Expansin is a cell wall loosing protein family that has four subfamilies:α-expansin(EXPA),β-expansin(EXPB),expansin-like A(EXLA),and expansin-like B(EXLB).In this study,we investigated the key period of pre-harvest texture formation in‘Golden Delicious’apples based on fruit longitudinal diameter,transverse diameter,firmness,tissue structure,respiration intensity,ethylene release rate,and expansin activity.Within the 10 days before harvest,the fruit was found to reach maturity.Semi-quantitative RT-PCR revealed that most of the expansins were expressed at the ripening stage before harvest.The biological function of the EXLB subfamily gene,MdEXLB1,was further identified,and its subcellular localization on the cell wall was confirmed by transient transformation experiments.Compared with the wild type(WT),the transgenic tomato lines overexpressing MdEXLB1 had lower plant height,earlier fruiting period,fewer days for fruit ripening,higher fruit maturity,lower fruit firmness,higher fruit expansin activity,more discrete flesh cell structure,and accelerated fruit ripening process.Overall,this is the first study to propose that the apple EXLB subfamily gene,MdEXLB1,has biological functions and plays an important role in promoting fruit ripening and softening.
基金supported by the National Social Science Fund of China (Grant No. 15CGL048)
文摘Based on 2012 input-output table of Shandong province and the official statistics, this article uses the input-output analysis method to build the input-output table. On the basis of the development of service industry in Shandong province's ability, we make a theoretical analysis framework of a comprehensive in-depth analysis on pull employment, creatively will cost tax rate, fully and completely labor capital and the combination of both the industry development space. So that the industry's ability to pull the employment can be considered, and to realize rapid and sustained development of service industry in Shandong province.
基金supported by the National Social Science Fundation of China(Grant No.15CGL048)。
文摘This paper puts forward the connotation of enterprise green management and green management performance evaluation,analyzes the characteristics of enterprise green management performance evaluation and decomposes its content.Using the triple performance method,the paper constructs the performance evaluation index system of enterprise green management.Taking BH Petrochemical Co.,Ltd.at the high-efficiency ecological economic zone of the Yellow River Delta as an example,by using analytic hierarchy process(AHP)and Matlab programming,this paper determines the weight of each index in the enterprise green management performance evaluation index system,the fuzzy comprehensive evaluation method is used to evaluate the green management performance evaluation of industrial enterprises.The results show that the performance evaluation index system of enterprise green management constructed by"triple performance method"has important theoretical significance and application value for evaluating and guiding enterprises to strengthen their green management.
