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田鼠巴贝斯虫棒状体蛋白相关因子1基因的原核表达及其特性研究 被引量:1
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作者 蒋艺佳 肖雯雯 +6 位作者 聂政 敖阳思琦 李慕晓 何沛 周金林 赵俊龙 贺兰 《中国动物传染病学报》 CAS 北大核心 2019年第2期48-54,共7页
本研究以田鼠巴贝斯虫基因组DNA为模板,扩增棒状体蛋白相关因子1(rhoptry-associated protein interacting factor 1,简称RAPIF1)基因,全长为771 bp,编码256 aa,编码蛋白的分子质量为29 kDa。利用多种数据库与生物信息学分析软件,对田... 本研究以田鼠巴贝斯虫基因组DNA为模板,扩增棒状体蛋白相关因子1(rhoptry-associated protein interacting factor 1,简称RAPIF1)基因,全长为771 bp,编码256 aa,编码蛋白的分子质量为29 kDa。利用多种数据库与生物信息学分析软件,对田鼠巴贝斯虫RAPIF1进行生物信息学分析,预测其亲水性/疏水性、跨膜区、信号肽、翻译后修饰位点、B淋巴细胞抗原表位,结果预示RAPIF1具有良好的亲水性。将RAPIF1的ORF插入表达载体pET-28a进行原核表达,重组蛋白rRAPIF1大小为37 kDa。制备rRAPIF1的多克隆抗体,Western blot结果显示,RAPIF1存在于虫体中,且rRAPIF1具有良好的反应原性,可有效区分田鼠巴贝斯虫阳性血清和阴性血清。本研究结果表明,RAPIF1预期可作为田鼠巴贝斯虫检测和疫苗制备的候选分子。 展开更多
关键词 田鼠巴贝斯虫 棒状体蛋白相关因子1 生物信息学分析 原核表达
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酶法催化柚皮苷制备根皮素及其抗氧化活性分析 被引量:2
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作者 李慧灵 周金林 +3 位作者 卢宇靖 陈宏基 叶德晓 林育成 《现代食品科技》 EI CAS 北大核心 2020年第12期69-76,共8页
本文阐述一种高效,环保制备高价值天然抗氧化剂根皮素的方法。以天然的柑橘提取物柚皮苷为原料,经一锅法加氢反应制得柚苷二氢查尔酮,再以α-鼠李糖苷酶(α-rhamnosidase)和纤维素酶(cellulase)高效催化水解而制得根皮素。采用单因素和... 本文阐述一种高效,环保制备高价值天然抗氧化剂根皮素的方法。以天然的柑橘提取物柚皮苷为原料,经一锅法加氢反应制得柚苷二氢查尔酮,再以α-鼠李糖苷酶(α-rhamnosidase)和纤维素酶(cellulase)高效催化水解而制得根皮素。采用单因素和正交实验对α-鼠李糖苷酶和纤维素酶酶解反应的p H、温度、酶与底物比进行优化,同时对根皮素、三叶苷、柚苷二氢查尔酮、柚苷的抗氧化性进行了研究,结果表明:α-鼠李糖苷酶(α-rhamnosidase)酶解最佳工艺为温度60℃、p H 4、酶与底物比为0.6:1;纤维素酶(cellulase)最佳工艺为温度45℃、p H 5.5、酶与底物比为0.3:1。在此工艺下根皮素的转化率为96.10%,比原工艺提高了10.53%。抗氧化性研究结果为:清除DPPH自由基能力根皮素(0.18±1.45)>三叶苷(0.3±1.63)>柚苷二氢查尔酮(0.95±1.24)>柚苷(8±1.97),还原铁离子能力为根皮素(7.1±0.68)>三叶苷(2.51±0.75)>柚苷二氢查尔酮(2.05±0.58)>柚苷(0.11±0.92)。本方法条件温和,周期短且转化率高,易于放大生产。抗氧化性结果表明根皮素具有较高的抗氧化性。这对根皮素的放大生产及应用具有重大意义。 展开更多
关键词 柚皮苷 酶法 根皮素 抗氧化性
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杜仲胶接枝对苯乙烯磺酸钠的研究 被引量:4
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作者 代丽 王文远 +2 位作者 周金琳 杨凤 方庆红 《沈阳化工大学学报》 CAS 2019年第2期145-150,共6页
采用乳液聚合工艺,以对苯乙烯磺酸钠(NaSS)为接枝单体,过硫酸铵[(NH4)2S2O8]为引发剂,成功地将对苯乙烯磺酸钠接枝到杜仲胶(EUG)的分子链上,制得磺化杜仲胶(mEUG).采用红外光谱仪(IR)、核磁共振氢谱仪(^1H-NMR)、示差量热扫描仪(DSC)、... 采用乳液聚合工艺,以对苯乙烯磺酸钠(NaSS)为接枝单体,过硫酸铵[(NH4)2S2O8]为引发剂,成功地将对苯乙烯磺酸钠接枝到杜仲胶(EUG)的分子链上,制得磺化杜仲胶(mEUG).采用红外光谱仪(IR)、核磁共振氢谱仪(^1H-NMR)、示差量热扫描仪(DSC)、偏光显微镜(POM)对磺化杜仲胶的结构和熔融-结晶行为进行表征.结果表明:通过这种方法可以成功地将磺酸基团引入到杜仲胶分子链中,接枝反应发生在双键邻近的亚甲基上.随着反应温度的提高,磺酸基团的引入量增加.磺酸基团的引入一方面破坏了杜仲胶分子链的对称性和规整性;但另一方面,离子基团之间的相互作用也促进了结晶速率和结晶温度的提高. 展开更多
关键词 杜仲胶 对苯乙烯磺酸钠 接枝共聚 磺化
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MOF-5衍生碳作为水系锌离子复合电容器阴极储能性能研究
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作者 黄彬 周锦林 +2 位作者 顾洪旭 于佳福 修志亮 《齐鲁工业大学学报》 2020年第4期9-12,共4页
采用锌金属有机配合物(MOF-5)煅烧得到的多孔碳材料作为阴极材料,以锌箔作为阳极,硫酸锌中系水溶液作为电解液构建了锌离子复合电容器。在电化学性能测试中,锌离子复合电容器表现出了优异的电化学性能,如高放电比容量(在1 A·g^-1... 采用锌金属有机配合物(MOF-5)煅烧得到的多孔碳材料作为阴极材料,以锌箔作为阳极,硫酸锌中系水溶液作为电解液构建了锌离子复合电容器。在电化学性能测试中,锌离子复合电容器表现出了优异的电化学性能,如高放电比容量(在1 A·g^-1的电流密度下放电比容量为55 mAh·g^-1),良好的倍率性能,高能量密度(46 Wh·kg^-1),优异的循环稳定性(在1 A·g^-1的电流密度下进行8000次充放电循环后,锌离子复合电容器的放电比容量保持率接近100%)。 展开更多
关键词 锌离子复合电容器 金属有机配合物 MOF-5 多孔碳 阴极
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Proteomic Analysis of Proteins in the Salivary Glands of the Fed and Unfed Female Tick Rhipicephalus haemaphysaloides
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作者 XIANG Fei-yu ZHANG Jian-wu +3 位作者 zhou Yong-zhi LI Zhuang GONG Hai-yan zhou jin-lin 《Agricultural Sciences in China》 CAS CSCD 2009年第1期121-127,共7页
Identification of differentially expressed salivary gland proteins between the fed and unfed female Rhipicephalus haemaphysaloides may obtain valuable functional molecules. In this study, comparative two-dimensional g... Identification of differentially expressed salivary gland proteins between the fed and unfed female Rhipicephalus haemaphysaloides may obtain valuable functional molecules. In this study, comparative two-dimensional gel electrophoresis (2-DE) and mass spectrometry were used to separate and identify differentially expressed salivary gland proteins between the fed and unfed female R. haemaphysaloides. The soluble proteins from the salivary glands of fed and unfed female R. haemaphysaloides were separated by a sequential extraction method followed by 2-DE and 2-DE images. Image analysis of the gels revealed 1 096 ± 87 protein spots from the fed female ticks and 991 ±64 protein spots from the unfed female ticks. Among those protein spots, about 724 ±34 were present both in the fed and unfed female ticks. Fourteen spots from the fed ticks and six spots from the unfed ticks were selected for peptide mass fingerprinting (PMF) and sequencing assay by mass spectrometry (MS). Bioinformatic analysis showed that a majority of the differentially expressed proteins were involved in signal transduction, metabolism, and transcriptional regulation. These differentially expressed proteins might be antigen candidates for the development of vaccines against the tick. 展开更多
关键词 Rhipicephalus haemaphysaloides salivary g/and differentially expressed proteins two-dimensional gel electrophoresis mass spectrometry
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The Midgut Bacterial Flora of Laboratory-Reared Hard Ticks, Haemaphysalis longicornis, Hyalomma asiaticum, and Rhipicephalus haemaphysaloides
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作者 LI Chun-hong CAO Jie +3 位作者 zhou Yong-zhi ZHANG Hou-shuang GONG Hai-yan zhou jin-lin 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2014年第8期1766-1771,共6页
Ixodid ticks play an important role in the transmission of a variety of zoonoses of viral, bacterial and protozoan origin, and they also harbor a wealth of microorganisms. To gain more detailed insights into the poten... Ixodid ticks play an important role in the transmission of a variety of zoonoses of viral, bacterial and protozoan origin, and they also harbor a wealth of microorganisms. To gain more detailed insights into the potential interactions between bacterial flora and tick-borne pathogens, we investigated the midgut bacterial flora of laboratory-reared Haemaphysalis longicornis, Hyalomma asiaticum and Rhipicephalus haemaphysaloides. Based on morphological, biochemical, and 16 S rDNA sequencing results, we identified 15 species belonging to 12 genera in the midgut of the three ticks. The bacterial communities were similar to those found in other studies of hematophagous arthropods. Kocuria sp. was the most frequently isolated species and its 16 S rDNA gene sequence was very similar to Kocuria koreensis P31 T. To our knowledge, this is the first report of the bacterial flora of tick midguts and the results show that there were many different bacterial species in each tick species. Among the most common genera, there may have been a novel species in the genus Kocuria. The results might be the first step for looking for different aspects of the pathogen and tick interaction. 展开更多
关键词 bacteria Kocuria sp. tick midgut
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