莱姆病(Lyme disease)又名伯氏疏螺旋体病,是20世纪70年代发现的以蜱为传播媒介,由不同基因型伯氏疏螺旋体(Borrelia burgdorferi sensu lato)感染所致的人兽共患自然疫源性疾病,主要流行于温带和亚热带地区,犬、猫、马、牛、鹿等多种...莱姆病(Lyme disease)又名伯氏疏螺旋体病,是20世纪70年代发现的以蜱为传播媒介,由不同基因型伯氏疏螺旋体(Borrelia burgdorferi sensu lato)感染所致的人兽共患自然疫源性疾病,主要流行于温带和亚热带地区,犬、猫、马、牛、鹿等多种动物易感,人也是该病原菌的易感宿主。人患莱姆病时的主要症状为游走性红斑、关节炎和其他神经系统症状。患病动物常表现的症状是蜱叮咬部位被毛脱落、发热、蹄叶炎、跛行等。莱姆病流行广泛,但是诊断较为困难并极易造成误诊和漏诊。为避免莱姆病对畜牧业以及宠物行业造成不利影响,亟需提高对莱姆病的关注。因此,本文对动物莱姆病及其诊断和防治技术的研究进展进行综述,以期为动物莱姆病的准确、快速诊断和防治提供参考。展开更多
Objective: To explore the therapeutic effect of Linggui Zhugan Decoction on nonalcoholic fatty liver disease through in vivo animal experiments, and to explore the mechanism of action of Linggui Zhugan Decoction in th...Objective: To explore the therapeutic effect of Linggui Zhugan Decoction on nonalcoholic fatty liver disease through in vivo animal experiments, and to explore the mechanism of action of Linggui Zhugan Decoction in the intervention of nonalcoholic fatty liver disease through network pharmacology and molecular docking technology. Methods: A rat model of non- alcoholic fatty liver disease was established after 20 weeks of high-fat feeding, and the rats were divided into six groups, normal group, model group, simvastatin group (4 mg/kg), low- dose group of Linggui Zhugan Decoction (2.1 g/kg), medium-dose group of Linggui Zhugan Decoction (4.2 g/kg), and high-dose group of Linggui Zhugan Decoction (8.4 g/kg), and the liver morphological changes of HE staining and oil red O staining after the intervention of Linggui Zhugan Decoction were observed, and the microplate reader detected aspartate aminotransferase, alanine aminotransferase, Four levels of blood lipids. Network pharmacology combined with molecular docking was used to screen the potential mechanism and target of Linggui Zhugan Decoction for the intervention of non-alcoholic fatty liver disease. Results: Linggui Zhugan Decoction significantly improved the levels of alanine aminotransferase and alanine aminotransferase in model rats (P<0.05). HE staining and oil red O staining showed that Linggui Zhugan Decoction significantly reduced liver lipid deposition in rats in the model group. Linggui Zhugan Decoction could significantly reduce the quadruplical levels of blood lipids in model rats (P<0.05). Network pharmacology screened out 10 key targets and 5 key signaling pathways;The molecular docking results showed that the first 20 active ingredients all had good binding ability to the top 10 targets. Conclusion: The results show that Linggui Zhugan Decoction has the effect of regulating blood lipids and improving liver tissue lesions in non-alcoholic fatty liver disease, and preliminarily verifies the regulatory effect of Linggui Zhugan Decoction on lipid metabolism-related genes.展开更多
文摘莱姆病(Lyme disease)又名伯氏疏螺旋体病,是20世纪70年代发现的以蜱为传播媒介,由不同基因型伯氏疏螺旋体(Borrelia burgdorferi sensu lato)感染所致的人兽共患自然疫源性疾病,主要流行于温带和亚热带地区,犬、猫、马、牛、鹿等多种动物易感,人也是该病原菌的易感宿主。人患莱姆病时的主要症状为游走性红斑、关节炎和其他神经系统症状。患病动物常表现的症状是蜱叮咬部位被毛脱落、发热、蹄叶炎、跛行等。莱姆病流行广泛,但是诊断较为困难并极易造成误诊和漏诊。为避免莱姆病对畜牧业以及宠物行业造成不利影响,亟需提高对莱姆病的关注。因此,本文对动物莱姆病及其诊断和防治技术的研究进展进行综述,以期为动物莱姆病的准确、快速诊断和防治提供参考。
基金Anhui Natural Science Foundat ion General Project(No.2208085MH277)。
文摘Objective: To explore the therapeutic effect of Linggui Zhugan Decoction on nonalcoholic fatty liver disease through in vivo animal experiments, and to explore the mechanism of action of Linggui Zhugan Decoction in the intervention of nonalcoholic fatty liver disease through network pharmacology and molecular docking technology. Methods: A rat model of non- alcoholic fatty liver disease was established after 20 weeks of high-fat feeding, and the rats were divided into six groups, normal group, model group, simvastatin group (4 mg/kg), low- dose group of Linggui Zhugan Decoction (2.1 g/kg), medium-dose group of Linggui Zhugan Decoction (4.2 g/kg), and high-dose group of Linggui Zhugan Decoction (8.4 g/kg), and the liver morphological changes of HE staining and oil red O staining after the intervention of Linggui Zhugan Decoction were observed, and the microplate reader detected aspartate aminotransferase, alanine aminotransferase, Four levels of blood lipids. Network pharmacology combined with molecular docking was used to screen the potential mechanism and target of Linggui Zhugan Decoction for the intervention of non-alcoholic fatty liver disease. Results: Linggui Zhugan Decoction significantly improved the levels of alanine aminotransferase and alanine aminotransferase in model rats (P<0.05). HE staining and oil red O staining showed that Linggui Zhugan Decoction significantly reduced liver lipid deposition in rats in the model group. Linggui Zhugan Decoction could significantly reduce the quadruplical levels of blood lipids in model rats (P<0.05). Network pharmacology screened out 10 key targets and 5 key signaling pathways;The molecular docking results showed that the first 20 active ingredients all had good binding ability to the top 10 targets. Conclusion: The results show that Linggui Zhugan Decoction has the effect of regulating blood lipids and improving liver tissue lesions in non-alcoholic fatty liver disease, and preliminarily verifies the regulatory effect of Linggui Zhugan Decoction on lipid metabolism-related genes.
