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LncRNA AL133467.1作为miR-661的ceRNA抑制乳腺癌细胞增殖和侵袭
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作者 王馨 黄嘉星 +3 位作者 周丽欢 陈伙娣 冯正富 邱惠思 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2022年第1期75-82,共8页
长非编码RNAs(long non-coding RNAs,lncRNAs)在多种肿瘤中异常表达并参与肿瘤的发生发展。然而,众多lncRNAs在肿瘤的表达及功能尚未完全阐明。本文通过分析TCGT数据库113例正常乳腺组织和1109例乳腺癌组织发现,LncRNA AL133467.1在乳... 长非编码RNAs(long non-coding RNAs,lncRNAs)在多种肿瘤中异常表达并参与肿瘤的发生发展。然而,众多lncRNAs在肿瘤的表达及功能尚未完全阐明。本文通过分析TCGT数据库113例正常乳腺组织和1109例乳腺癌组织发现,LncRNA AL133467.1在乳腺癌组织中低表达,并与乳腺癌患者的预后不良呈负相关;实时荧光定量PCR(qRT-PCR)结果也发现,AL133467.1在乳腺癌细胞中表达明显低于正常乳腺上皮细胞。在相对低表达的乳腺癌细胞SKBR3和BT474中过表达AL133467.1,细胞计数和平板克隆结果发现,过表达AL133467.1能明显抑制乳腺癌细胞的增殖和克隆形成能力(P<0.01);细胞划痕和Transwell实验显示,与对照组相比,过表达AL133467.1的乳腺癌细胞的迁移和侵袭能力明显降低(P<0.01)。miRDB数据库显示,AL133467.1与miR-661存在结合位点,miR-661可以靶向结合ErbB2受体酪氨酸激酶2传感器2(transducer of ERBB2,2,TOB2);qRT-PCR结果显示,miR-661在乳腺癌细胞中高表达,并且与乳腺癌患者的预后不良呈正相关(P<0.001);荧光素酶报告基因结果显示,AL133467.1与miR-661存在特异性结合(P<0.01),过表达AL133467.1能抑制乳腺癌细胞中miR-661的表达(P<0.0001);同时,转染miR-661 mimics能消除过表达AL133467.1对乳腺癌细胞的抑制侵袭作用(P<0.001);此外,qRT-PCR和Western印迹结果显示,过表达AL133467.1能上调TOB2的mRNA(P<0.0001)和蛋白质水平,同时转染miR-661 mimics乳腺癌细胞中TOB2的mRNA(P<0.0001)和蛋白质水平明显被抑制。综上所述,AL133467.1作为miR-661的竞争性RNA上调TOB2的表达,从而抑制乳腺癌细胞增殖和侵袭。 展开更多
关键词 长非编码RNA miR-661 酪氨酸激酶2传感器2 增殖 侵袭 乳腺癌
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Quark, Gluon, Odderon Contributions to Total Cross Section of Proton-Proton Elastic Scattering at High Energies
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作者 LU Juan zhou li-huan +1 位作者 MA Wei-Xing HE Xiao-Rong 《Communications in Theoretical Physics》 SCIE CAS CSCD 2008年第1期207-211,共5页
Based on the quark-gluon structure of nucleon and the existence of Odderon in nucleon via gluon selfinteraction, the elastic scattering of pp at high energies is studied. Our theoretical predictions reproduce experime... Based on the quark-gluon structure of nucleon and the existence of Odderon in nucleon via gluon selfinteraction, the elastic scattering of pp at high energies is studied. Our theoretical predictions reproduce experimental data perfectly. The contributions from individual terms of quark-quark, gluon-gluon interactions, quark-gluon interference and the Odderon terms to total cross section are analyzed. In addition to the leading quark-quark contribution, the Odderon contribution is quite important. In particular, the Odderon plays an essential role in fitting to data. Therefore, We may claim that the high energy pp and pp elastic scattering may be good processes to search for the Odderon, the three Reggeized gluon bound states. 展开更多
关键词 pp elastic scattering QUARK GLUON Odderons
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