目的:研究巨噬细胞迁移抑制因子(MIF)对氧糖剥夺/复氧(OGD/R)条件下PC12细胞的保护作用及其机制。方法:将PC12细胞分为对照组(control)、氧糖剥夺/复氧组(OGD/R)、MIF处理组(MIF)、ISO-1处理组(ISO-1)和MIF联合MIF抑制剂ISO-1处理组(ISO...目的:研究巨噬细胞迁移抑制因子(MIF)对氧糖剥夺/复氧(OGD/R)条件下PC12细胞的保护作用及其机制。方法:将PC12细胞分为对照组(control)、氧糖剥夺/复氧组(OGD/R)、MIF处理组(MIF)、ISO-1处理组(ISO-1)和MIF联合MIF抑制剂ISO-1处理组(ISO-1+MIF)。利用无糖无血清培养液及低氧处理PC12细胞建立OGD/R诱导的神经元损伤模型,利用CCK-8试剂盒检测细胞活力,利用DCFH-DA探针检测胞内ROS水平;利用乳酸测试盒检测细胞培养上清中的乳酸水平;real time RT-PCR检测细胞中Bax,Bcl-2和葡萄糖转运蛋白1(GLUT1)mRNA的表达;Western Blot检测细胞中Bax,Bcl-2,GLUT1,乳酸脱氢酶A(LDHA)和M2型丙酮酸激酶(PKM2)蛋白的表达;免疫荧光染色检测细胞中凋亡相关蛋白caspase-3,Bax和Bcl-2的表达变化。结果:与control组相比,OGD/R组细胞活性降低,凋亡增加。同时,OGD/R降低了葡萄糖代谢相关蛋白GLUT1,PKM2和LDHA的表达,降低了乳酸的形成量并增加了ROS生成。MIF的给药促进了OGD/R下PC12细胞的活力并抑制其凋亡,增加了GLUT1,PKM2和LDHA蛋白表达以及乳酸含量,并且降低了ROS水平。相反,ISO-1可以抑制MIF对PC12细胞的保护作用。结论:MIF通过发挥抗凋亡、抗氧化应激和增加葡萄糖代谢对PC12细胞发挥保护作用。展开更多
An easy-to-implement method by which to fabricate superhydrophobic surfaces inspired taro leaf was successfully applied on316 L stainless steel via combining nanosecond laser(NL)processing and spin-coating techniques....An easy-to-implement method by which to fabricate superhydrophobic surfaces inspired taro leaf was successfully applied on316 L stainless steel via combining nanosecond laser(NL)processing and spin-coating techniques.The laser-textured surface composed of microscale frameworks and central bumps was fabricated by NL processing based on properly designed biomimetic patterns,and a layer of nanoscale carbon black/polydimethylsiloxane(CB/PDMS)particles was covered on it by spin-coating.The effect of pattern parameters(i.e.,the inscribed circle radius of framework and the radius of central bump)on wettability of biomimetic surface was investigated.All as-prepared biomimetic surfaces with micro-nano hierarchical structures showed excellent superhydrophobicity with the water contact angle of~155°and contact angle hysteresis of~2°.By comparing the untreated surface,the wetting behavior and evaporation mode of the biomimetic surface occurred an obvious transformation.Meanwhile,experiments indicated that the biomimetic surface not only had liquid-repelling and self-cleaning functions,but also maintained remarkable mechanical robustness and superhydrophobic durability.The method is efficient for fabricating biomimetic superhydrophobic surfaces applied to liquid-repelling,evaporation-transforming and self-cleaning fields.展开更多
文摘目的:研究巨噬细胞迁移抑制因子(MIF)对氧糖剥夺/复氧(OGD/R)条件下PC12细胞的保护作用及其机制。方法:将PC12细胞分为对照组(control)、氧糖剥夺/复氧组(OGD/R)、MIF处理组(MIF)、ISO-1处理组(ISO-1)和MIF联合MIF抑制剂ISO-1处理组(ISO-1+MIF)。利用无糖无血清培养液及低氧处理PC12细胞建立OGD/R诱导的神经元损伤模型,利用CCK-8试剂盒检测细胞活力,利用DCFH-DA探针检测胞内ROS水平;利用乳酸测试盒检测细胞培养上清中的乳酸水平;real time RT-PCR检测细胞中Bax,Bcl-2和葡萄糖转运蛋白1(GLUT1)mRNA的表达;Western Blot检测细胞中Bax,Bcl-2,GLUT1,乳酸脱氢酶A(LDHA)和M2型丙酮酸激酶(PKM2)蛋白的表达;免疫荧光染色检测细胞中凋亡相关蛋白caspase-3,Bax和Bcl-2的表达变化。结果:与control组相比,OGD/R组细胞活性降低,凋亡增加。同时,OGD/R降低了葡萄糖代谢相关蛋白GLUT1,PKM2和LDHA的表达,降低了乳酸的形成量并增加了ROS生成。MIF的给药促进了OGD/R下PC12细胞的活力并抑制其凋亡,增加了GLUT1,PKM2和LDHA蛋白表达以及乳酸含量,并且降低了ROS水平。相反,ISO-1可以抑制MIF对PC12细胞的保护作用。结论:MIF通过发挥抗凋亡、抗氧化应激和增加葡萄糖代谢对PC12细胞发挥保护作用。
基金the National Key Research and Development Program of China(Grant No.2019YFE0126300)the National Natural Science Foundation of China(Grant No.51775197)the Natural Science Foundation of Guangdong Province(Grant No.2019A1515011530)。
文摘An easy-to-implement method by which to fabricate superhydrophobic surfaces inspired taro leaf was successfully applied on316 L stainless steel via combining nanosecond laser(NL)processing and spin-coating techniques.The laser-textured surface composed of microscale frameworks and central bumps was fabricated by NL processing based on properly designed biomimetic patterns,and a layer of nanoscale carbon black/polydimethylsiloxane(CB/PDMS)particles was covered on it by spin-coating.The effect of pattern parameters(i.e.,the inscribed circle radius of framework and the radius of central bump)on wettability of biomimetic surface was investigated.All as-prepared biomimetic surfaces with micro-nano hierarchical structures showed excellent superhydrophobicity with the water contact angle of~155°and contact angle hysteresis of~2°.By comparing the untreated surface,the wetting behavior and evaporation mode of the biomimetic surface occurred an obvious transformation.Meanwhile,experiments indicated that the biomimetic surface not only had liquid-repelling and self-cleaning functions,but also maintained remarkable mechanical robustness and superhydrophobic durability.The method is efficient for fabricating biomimetic superhydrophobic surfaces applied to liquid-repelling,evaporation-transforming and self-cleaning fields.
