栽培六倍体燕麦是世界重要粮食作物,理清其起源对燕麦种质资源的高效利用和保护具有重要意义。本研究利用GBS(genotyping by sequencing)对27份来自中国的大粒裸燕麦材料测序,结合先前发表的包括6个六倍体燕麦种在内的66份燕麦材料的GB...栽培六倍体燕麦是世界重要粮食作物,理清其起源对燕麦种质资源的高效利用和保护具有重要意义。本研究利用GBS(genotyping by sequencing)对27份来自中国的大粒裸燕麦材料测序,结合先前发表的包括6个六倍体燕麦种在内的66份燕麦材料的GBS数据进行SNP挖掘。UNEAK管道挖掘共计得到MAF大于0.5,call rate大于0.95的SNP标记8902个。进一步剔除缺失值大于0.15的4个燕麦材料后,对其余89份材料进行PCA分析、STRUCTURE分析以及UPGMA聚类分析。结果表明,在野生种中,除A.sterilis外,大多数来自同一物种的材料聚为一类,不同物种间能够较好地分开,表明这些物种之间存在较强的遗传分化。聚类分析将供试材料分为分别代表野生种和栽培种的2支,表明野生种和栽培种之间存在明显的遗传差异;在栽培种中,A.sativa与A.byzantina具有较高的遗传多样性,分散在不同的类群中,二者未出现明显的遗传分化,具有较高的遗传同质性,A.sativa ssp.nuda与A.sativa亲缘关系较近,但存在一定的遗传分化,因此形成独立的类群。值得注意的是,来自野生种A.sterilis的材料被分在2个类群中,其中来自西南亚地区(伊朗-伊拉克-土耳其地区)的居群与A.sativa和A.byzantina聚在一起,揭示此地区的A.sterilis居群可能是A.sativa和A.byzantina的祖先种。野生种A.hybrida显示出与A.fatua较高的遗传同质性,因此将其作为A.fatua的亚种较为合理。本研究为栽培六倍体燕麦起源提供了理论依据。展开更多
Objective To identify specific Chinese medicines(CMs)that may benefit patients with gastroesophageal reflux disease(GERD),and explore the action mechanism.Methods Domestic and foreign literature on the treatment of GE...Objective To identify specific Chinese medicines(CMs)that may benefit patients with gastroesophageal reflux disease(GERD),and explore the action mechanism.Methods Domestic and foreign literature on the treatment of GERD with CMs was searched and selected from China National Knowledge Infrastructure,China Science and Technology Journal Database,Wanfang Database,and PubMed from October 1,2011 to October 1,2021.Data from all eligible articles were extracted to establish the database of CMs for GERD.Apriori algorithm of data mining techniques was used to analyze the rules of herbs selection and core Chinese medicine formulas were identified.A system pharmacology approach was used to explore the action mechanism of these medicines.Results A total of 278 prescriptions for GERD were analyzed,including 192 CMs.Results of Apriori algorithm indicated that Evodiae Fructus and Coptidis Rhizoma were the highest confidence combination.A total of 32 active ingredients and 66 targets were screened for the treatment of GERD.Enrichment analysis showed that the mechanisms of action mainly involved pathways in cancer,fluid shear stress and atherosclerosis,advanced glycation end product(AGE),the receptor for AGE signaling pathway in diabetic complications,bladder cancer,and rheumatoid arthritis.Conclusion Evodiae Fructus and Coptidis Rhizoma are the core drugs in the treatment of GERD and the potential mechanism of action of these medicines includes potential target and pathways.展开更多
文摘栽培六倍体燕麦是世界重要粮食作物,理清其起源对燕麦种质资源的高效利用和保护具有重要意义。本研究利用GBS(genotyping by sequencing)对27份来自中国的大粒裸燕麦材料测序,结合先前发表的包括6个六倍体燕麦种在内的66份燕麦材料的GBS数据进行SNP挖掘。UNEAK管道挖掘共计得到MAF大于0.5,call rate大于0.95的SNP标记8902个。进一步剔除缺失值大于0.15的4个燕麦材料后,对其余89份材料进行PCA分析、STRUCTURE分析以及UPGMA聚类分析。结果表明,在野生种中,除A.sterilis外,大多数来自同一物种的材料聚为一类,不同物种间能够较好地分开,表明这些物种之间存在较强的遗传分化。聚类分析将供试材料分为分别代表野生种和栽培种的2支,表明野生种和栽培种之间存在明显的遗传差异;在栽培种中,A.sativa与A.byzantina具有较高的遗传多样性,分散在不同的类群中,二者未出现明显的遗传分化,具有较高的遗传同质性,A.sativa ssp.nuda与A.sativa亲缘关系较近,但存在一定的遗传分化,因此形成独立的类群。值得注意的是,来自野生种A.sterilis的材料被分在2个类群中,其中来自西南亚地区(伊朗-伊拉克-土耳其地区)的居群与A.sativa和A.byzantina聚在一起,揭示此地区的A.sterilis居群可能是A.sativa和A.byzantina的祖先种。野生种A.hybrida显示出与A.fatua较高的遗传同质性,因此将其作为A.fatua的亚种较为合理。本研究为栽培六倍体燕麦起源提供了理论依据。
基金Supported by the Natural Science Foundation of Hebei(No.H2020423207)the Postgraduate Innovation Funding Project of Hebei University of Chinese Medicine(No.XCXZZSS2022023)。
文摘Objective To identify specific Chinese medicines(CMs)that may benefit patients with gastroesophageal reflux disease(GERD),and explore the action mechanism.Methods Domestic and foreign literature on the treatment of GERD with CMs was searched and selected from China National Knowledge Infrastructure,China Science and Technology Journal Database,Wanfang Database,and PubMed from October 1,2011 to October 1,2021.Data from all eligible articles were extracted to establish the database of CMs for GERD.Apriori algorithm of data mining techniques was used to analyze the rules of herbs selection and core Chinese medicine formulas were identified.A system pharmacology approach was used to explore the action mechanism of these medicines.Results A total of 278 prescriptions for GERD were analyzed,including 192 CMs.Results of Apriori algorithm indicated that Evodiae Fructus and Coptidis Rhizoma were the highest confidence combination.A total of 32 active ingredients and 66 targets were screened for the treatment of GERD.Enrichment analysis showed that the mechanisms of action mainly involved pathways in cancer,fluid shear stress and atherosclerosis,advanced glycation end product(AGE),the receptor for AGE signaling pathway in diabetic complications,bladder cancer,and rheumatoid arthritis.Conclusion Evodiae Fructus and Coptidis Rhizoma are the core drugs in the treatment of GERD and the potential mechanism of action of these medicines includes potential target and pathways.
