采用常压室温等离子体(Atmospheric and room temperature plasma,ARTP)诱变苏云金芽孢杆菌Bt-NBIC-380菌株,选育对亚洲玉米螟高毒力突变株。经过4轮的ARTP诱变、镜检及摇瓶发酵筛选得到的突变株Bt-NBIC-380-18-407,其摇瓶发酵液对玉米...采用常压室温等离子体(Atmospheric and room temperature plasma,ARTP)诱变苏云金芽孢杆菌Bt-NBIC-380菌株,选育对亚洲玉米螟高毒力突变株。经过4轮的ARTP诱变、镜检及摇瓶发酵筛选得到的突变株Bt-NBIC-380-18-407,其摇瓶发酵液对玉米螟二龄幼虫效价达到6 835 IU/μL,杀虫晶体蛋白含量6.3 mg/mL。该突变株的效价较初始菌株提高了25.7%,杀虫晶体蛋白含量提高了18.9%。经过5次传代,证实该突变株具有良好的遗传稳定性。展开更多
This study is to investigate the role of lipid transfer protein （LTP1） gene of wheat （Triticum aestivum L.） in powdery mildew （Blumeria graminis f.sp. tritici, Bgt） resistance. A pair of primers based on the ful...This study is to investigate the role of lipid transfer protein （LTP1） gene of wheat （Triticum aestivum L.） in powdery mildew （Blumeria graminis f.sp. tritici, Bgt） resistance. A pair of primers based on the full length cDNA of wheat LTP1 was used for amplifying the coding regions of LTP in hexaploid （AABBDD） wheat and its diploid donors T. urartu （AA）, Ae. speltoides ssp speltoide （SS） and Ae. tauchii ssp strangulate （DD）. LTP1 and LTP2 of wheat were isolated from the tested two hexaploid （ABD） materials： powdery mildew resistance near isogenic line （NIL） Mardler/7 × Bainong 3217 and its susceptible parent Bainong 3217 at the same time, while only one kind ofLTP gene was found in the tested three diploid materials respectively by using the above PCR primer pairs. Two peaks of the expression of LTP1 and LTP2 induced by powdery mildew were observed [one occurred at 3 h after inoculation （hai）; the other occurred at 10 hai] in resistant NIL Mardler/7 × Bainong3217 in comparison with a steady transcript level of LTP1 and LTP2 in susceptible Bainong3217. Transient over-expression result showed that LTP1 reduced the penetration efficiency （PE） of powdery mildew in susceptible cultivar by about 28.3%. This result indicated an obvious effectiveness of LTP1 in powdery mildew resistance. Expression analysis also showed that LTP1 and LTP2 of wheat are generally involved in salt/drought, but not in low temperature stress early responses.展开更多
文摘采用常压室温等离子体(Atmospheric and room temperature plasma,ARTP)诱变苏云金芽孢杆菌Bt-NBIC-380菌株,选育对亚洲玉米螟高毒力突变株。经过4轮的ARTP诱变、镜检及摇瓶发酵筛选得到的突变株Bt-NBIC-380-18-407,其摇瓶发酵液对玉米螟二龄幼虫效价达到6 835 IU/μL,杀虫晶体蛋白含量6.3 mg/mL。该突变株的效价较初始菌株提高了25.7%,杀虫晶体蛋白含量提高了18.9%。经过5次传代,证实该突变株具有良好的遗传稳定性。
文摘This study is to investigate the role of lipid transfer protein （LTP1） gene of wheat （Triticum aestivum L.） in powdery mildew （Blumeria graminis f.sp. tritici, Bgt） resistance. A pair of primers based on the full length cDNA of wheat LTP1 was used for amplifying the coding regions of LTP in hexaploid （AABBDD） wheat and its diploid donors T. urartu （AA）, Ae. speltoides ssp speltoide （SS） and Ae. tauchii ssp strangulate （DD）. LTP1 and LTP2 of wheat were isolated from the tested two hexaploid （ABD） materials： powdery mildew resistance near isogenic line （NIL） Mardler/7 × Bainong 3217 and its susceptible parent Bainong 3217 at the same time, while only one kind ofLTP gene was found in the tested three diploid materials respectively by using the above PCR primer pairs. Two peaks of the expression of LTP1 and LTP2 induced by powdery mildew were observed [one occurred at 3 h after inoculation （hai）; the other occurred at 10 hai] in resistant NIL Mardler/7 × Bainong3217 in comparison with a steady transcript level of LTP1 and LTP2 in susceptible Bainong3217. Transient over-expression result showed that LTP1 reduced the penetration efficiency （PE） of powdery mildew in susceptible cultivar by about 28.3%. This result indicated an obvious effectiveness of LTP1 in powdery mildew resistance. Expression analysis also showed that LTP1 and LTP2 of wheat are generally involved in salt/drought, but not in low temperature stress early responses.
