背景面中部骨折导致的慢性泪囊炎病情复杂,普通泪道CT造影检查难以清晰准确地显示双侧鼻泪管全程。基于骨性鼻泪管走向的泪道CT造影三平面重组技术(three-plane reconstruction of CT dacryocystography based on the trend of nasolacr...背景面中部骨折导致的慢性泪囊炎病情复杂,普通泪道CT造影检查难以清晰准确地显示双侧鼻泪管全程。基于骨性鼻泪管走向的泪道CT造影三平面重组技术(three-plane reconstruction of CT dacryocystography based on the trend of nasolacrimal canal,CT-DCG-TPR-BTNC)可更清晰地显示泪道组织结构细节。目的探讨CT-DCG-TPR-BTNC技术在面中部骨折致外伤性慢性泪囊炎的诊断及治疗中的应用效果。方法回顾性分析2019年6月-2021年12月就诊于解放军总医院第三医学中心的22例外伤性慢性泪囊炎患者的临床及CT资料,患者均经过CT-DCG-TPR-BTNC检查,分析面中部骨折对泪道阻塞的影响,并在术中验证CT-DCG-TPR-BTNC检查结果的一致情况。结果22例患者中,男性10例(45.5%),女性12例(54.5%)。年龄6~53(30.86±12.02)岁。22例患者均患有外伤性慢性泪囊炎。病史3~180(37.32±52.95)个月。所有患者经CT-DCG-TPR-BTNC检查发现均有面中部骨折及其导致的鼻泪管骨折。面中部骨折包括鼻骨骨折(21/22,95.5%)、眶壁骨折(19/22,86.4%)、颌面骨折(12/22,54.5%)、泪囊萎缩(2/22,9.1%),并有1例颅底骨折。所有患者均行外路改良泪囊鼻腔吻合术,术中所见与CT-DCG-TPR-BTNC检查结果完全一致,符合率100%。患者均术中置入双泪小管置入式人工泪管,术后3~6个月取出人工泪管,随诊3~6个月后无复发。结论应用CT-DCG-TPR-BTNC技术术前可充分评估临近组织的损伤,尤其是骨性鼻泪管损伤和解剖变异情况,可评估泪囊窝及鼻泪管损伤程度,对于指导手术方案的制订有重要临床价值。展开更多
OBJECTIVE:To uncover the anti-tumor effects and potential mechanism of Sanwu Baisan Decoction(三物白散,SWB)in treatment of colorectal cancer(CRC)in mice.METHODS:Therapeutic effect was evaluated based on body weight ga...OBJECTIVE:To uncover the anti-tumor effects and potential mechanism of Sanwu Baisan Decoction(三物白散,SWB)in treatment of colorectal cancer(CRC)in mice.METHODS:Therapeutic effect was evaluated based on body weight gain,tumor volume,tumor growth inhibition rate,and histological changes and apoptosis in the tumor tissues.Anti-tumor immunity was studied by measuring plasma levels of anti-tumor cytokines,interleukin 6(IL-6),interleukin 17(IL-17),and interferonγ(IFN-γ).Gut morphological changes were evaluated by histological staining and tight junction proteins expressions.Gut microbiota composition was analyzed by 16S r RNA gene sequencing.Classical toll-like receptor 4(TLR-4)/cyclooxygenase 2(COX-2)/prostaglandin E2(PGE-2)pathway was examined in colon tissue and tumor samples.RESULTS:SWB presented high anti-tumor efficacy of CRC in mice,which manifested as decreased tumor volume and increased tumor growth inhibition rate.This anti-tumor effect of SWB was associated with elevated plasma levels of anti-tumor immune cytokines(IL-6,IL-17,and IFN-γ).Further studies showed that SWB also increases the expression of occluding and promotes the abundance of gut probiotics,Clostridium_XIVa,Enterorhabdus,and uncultured_bacterium.Moreover,results suggested that the anti-tumor effects of SWB might associate with inducing cancer cell apoptosis and inhibiting the TLR-4/COX-2/PGE-2 pathway in both colon tissue and tumor samples.CONCLUSION:SWB shows strong anti-tumor efficiency in mice with colorectal carcinoma,possibly through promoting the secretion of anti-tumor immune cytokines,inducing cancer apoptosis,maintaining the gut microbiota,and inhibiting tumorigenesis by inhibiting the TLR-4/COX-2/PGE-2 pathway.展开更多
文摘背景面中部骨折导致的慢性泪囊炎病情复杂,普通泪道CT造影检查难以清晰准确地显示双侧鼻泪管全程。基于骨性鼻泪管走向的泪道CT造影三平面重组技术(three-plane reconstruction of CT dacryocystography based on the trend of nasolacrimal canal,CT-DCG-TPR-BTNC)可更清晰地显示泪道组织结构细节。目的探讨CT-DCG-TPR-BTNC技术在面中部骨折致外伤性慢性泪囊炎的诊断及治疗中的应用效果。方法回顾性分析2019年6月-2021年12月就诊于解放军总医院第三医学中心的22例外伤性慢性泪囊炎患者的临床及CT资料,患者均经过CT-DCG-TPR-BTNC检查,分析面中部骨折对泪道阻塞的影响,并在术中验证CT-DCG-TPR-BTNC检查结果的一致情况。结果22例患者中,男性10例(45.5%),女性12例(54.5%)。年龄6~53(30.86±12.02)岁。22例患者均患有外伤性慢性泪囊炎。病史3~180(37.32±52.95)个月。所有患者经CT-DCG-TPR-BTNC检查发现均有面中部骨折及其导致的鼻泪管骨折。面中部骨折包括鼻骨骨折(21/22,95.5%)、眶壁骨折(19/22,86.4%)、颌面骨折(12/22,54.5%)、泪囊萎缩(2/22,9.1%),并有1例颅底骨折。所有患者均行外路改良泪囊鼻腔吻合术,术中所见与CT-DCG-TPR-BTNC检查结果完全一致,符合率100%。患者均术中置入双泪小管置入式人工泪管,术后3~6个月取出人工泪管,随诊3~6个月后无复发。结论应用CT-DCG-TPR-BTNC技术术前可充分评估临近组织的损伤,尤其是骨性鼻泪管损伤和解剖变异情况,可评估泪囊窝及鼻泪管损伤程度,对于指导手术方案的制订有重要临床价值。
基金Supported by National Natural Science Foundation-funded Project:the Biological Mechanism of Lingguizhugan Decoction Treating Alzheimer’s Disease via Regulating Brain Lymphatic Drainage System(No.82074504)the Priority Academic Program Development of Jiangsu Higher Education Institutions(Integration of Chinese and Western Medicine)Natural Science Foundation of the Jiangsu Higher Education Institutions-funded Project:to Explore the Mechanism of Sanwubai San Regulating Gut Microbiota Against Colorectal Cancer By IL-23/ΓδT17/IL-17 Axis(19KJB360003)。
文摘OBJECTIVE:To uncover the anti-tumor effects and potential mechanism of Sanwu Baisan Decoction(三物白散,SWB)in treatment of colorectal cancer(CRC)in mice.METHODS:Therapeutic effect was evaluated based on body weight gain,tumor volume,tumor growth inhibition rate,and histological changes and apoptosis in the tumor tissues.Anti-tumor immunity was studied by measuring plasma levels of anti-tumor cytokines,interleukin 6(IL-6),interleukin 17(IL-17),and interferonγ(IFN-γ).Gut morphological changes were evaluated by histological staining and tight junction proteins expressions.Gut microbiota composition was analyzed by 16S r RNA gene sequencing.Classical toll-like receptor 4(TLR-4)/cyclooxygenase 2(COX-2)/prostaglandin E2(PGE-2)pathway was examined in colon tissue and tumor samples.RESULTS:SWB presented high anti-tumor efficacy of CRC in mice,which manifested as decreased tumor volume and increased tumor growth inhibition rate.This anti-tumor effect of SWB was associated with elevated plasma levels of anti-tumor immune cytokines(IL-6,IL-17,and IFN-γ).Further studies showed that SWB also increases the expression of occluding and promotes the abundance of gut probiotics,Clostridium_XIVa,Enterorhabdus,and uncultured_bacterium.Moreover,results suggested that the anti-tumor effects of SWB might associate with inducing cancer cell apoptosis and inhibiting the TLR-4/COX-2/PGE-2 pathway in both colon tissue and tumor samples.CONCLUSION:SWB shows strong anti-tumor efficiency in mice with colorectal carcinoma,possibly through promoting the secretion of anti-tumor immune cytokines,inducing cancer apoptosis,maintaining the gut microbiota,and inhibiting tumorigenesis by inhibiting the TLR-4/COX-2/PGE-2 pathway.
