为了提升发酵法产L-色氨酸的生产效率,使用常压室温等离子(atmospheric and room temperature plasma,ARTP)诱变育种技术,并结合结构类似物抗性定向筛选的方法,选育高产色氨酸菌株。将出发菌Escherichia coli AC-1042经过ARTP诱变处理后...为了提升发酵法产L-色氨酸的生产效率,使用常压室温等离子(atmospheric and room temperature plasma,ARTP)诱变育种技术,并结合结构类似物抗性定向筛选的方法,选育高产色氨酸菌株。将出发菌Escherichia coli AC-1042经过ARTP诱变处理后,在抗性培养基平板上筛选具有5-甲基色氨酸、对氟苯丙氨酸抗性的突变菌株,选取产酸高、遗传稳定的菌株重复进行ARTP诱变处理和抗性筛选,不断提高菌株对结构类似物的抗性水平。经过多次ARTP诱变处理和抗性筛选,获得1株色氨酸高产菌株ACTRP104,经过30 L发酵罐培养44 h后L-色氨酸质量浓度可以达到61.65 g/L,葡萄糖转化率达到20.64%,比出发菌分别提高了20.69%和17.81%。结果表明,ARTP诱变和结构类似物抗性筛选相结合,可以有效地获得色氨酸高产突变菌株,大大提高色氨酸的发酵生产技术水平,获得的色氨酸高产菌株ACTRP104具有较好的工业化应用前景。展开更多
A self adaptive three-dimensional baroclinic model is designed. A horizontal temperature gradient is used to control the grid size, which can improve computational precision in the fronts without inordinately increasi...A self adaptive three-dimensional baroclinic model is designed. A horizontal temperature gradient is used to control the grid size, which can improve computational precision in the fronts without inordinately increasing computation in the whole area. A simulation of the development and disappearance of the front in the Huanghai Sea is conducted with this model. Simulations of temperature distribution throughout the year are also conducted. The comoutational result agrees well with the observation.展开更多
文摘为了提升发酵法产L-色氨酸的生产效率,使用常压室温等离子(atmospheric and room temperature plasma,ARTP)诱变育种技术,并结合结构类似物抗性定向筛选的方法,选育高产色氨酸菌株。将出发菌Escherichia coli AC-1042经过ARTP诱变处理后,在抗性培养基平板上筛选具有5-甲基色氨酸、对氟苯丙氨酸抗性的突变菌株,选取产酸高、遗传稳定的菌株重复进行ARTP诱变处理和抗性筛选,不断提高菌株对结构类似物的抗性水平。经过多次ARTP诱变处理和抗性筛选,获得1株色氨酸高产菌株ACTRP104,经过30 L发酵罐培养44 h后L-色氨酸质量浓度可以达到61.65 g/L,葡萄糖转化率达到20.64%,比出发菌分别提高了20.69%和17.81%。结果表明,ARTP诱变和结构类似物抗性筛选相结合,可以有效地获得色氨酸高产突变菌株,大大提高色氨酸的发酵生产技术水平,获得的色氨酸高产菌株ACTRP104具有较好的工业化应用前景。
基金The National Science and Technology Support Program of China under contract No.2011BAC03B02the National Natural Science Foundation of China under contract No. 40976002
文摘A self adaptive three-dimensional baroclinic model is designed. A horizontal temperature gradient is used to control the grid size, which can improve computational precision in the fronts without inordinately increasing computation in the whole area. A simulation of the development and disappearance of the front in the Huanghai Sea is conducted with this model. Simulations of temperature distribution throughout the year are also conducted. The comoutational result agrees well with the observation.