Heavy metal contamination of soil has become a major environmental concern worldwide over the past several decades,the exploitation and application of heavy metal hyperaccumulating plants will defend the safety of foo...Heavy metal contamination of soil has become a major environmental concern worldwide over the past several decades,the exploitation and application of heavy metal hyperaccumulating plants will defend the safety of food and environment on which human life relied,whereas using natural hyperaccumulators is insufficient,due展开更多
Aim: To study the expression of MMP1 and TIMP1 in normal and radiation-combined wound healing and their effects on the healing process. Materials and Methods: A rat model of radiation-combined wound healing was used, ...Aim: To study the expression of MMP1 and TIMP1 in normal and radiation-combined wound healing and their effects on the healing process. Materials and Methods: A rat model of radiation-combined wound healing was used, and routine light microscopy, electron microscopy, immunohistochemistry, and in situ hybridization were used to detect the expression of MMP1 and TIMP1 during the healing process. Results: The wound healing process was impaired and delayed. In rats receiving 25Gy Gamma ray locally, the irradiated wounds healed 6 days later than non-irradiated controls. The following changes were found in the expression of MMP1 and TIMP1: (1) In the early inflammatory phase and in the period of granulation tissue formation, MMP1 expression was only slightly if at all affected in the newly-formed epidermis of irradiated wounds when compared with that in controls. Later, the epidermal expression of MMP1 in irradiated wounds was comparatively increased following the delayed healing process. 3 to 14 days after wounding, TIMP1 was weakly positive in proliferating keratinocytes of control group and became negative after epidermal covering, whereas no or only slight epidermal expression was detected in the irradiated group before epidermal covering. (2) The expression of MMP1 and TIMP1 in irradiated group was markedly decreased in fibroblasts, endotheliocytes and macrophages when compared to that in controls. The expression phase was prolonged due to the delayed healing process. Conclusions: It is concluded that the reduced expression of MMP1 and TIMP1 in granulation tissue retards such important processes as cell migration and angiogenesis, thus slowing the healing process. The expression of MMP1 in the newly-formed epidermis may help the process of reepithelialization, but in the late healing period, overexpression of MMP1 and decreased expression of TIMP1 in the epidermis may hinder the establishment of basal membrane and scar formation.展开更多
Background Recently, arsenic trioxide (As 2O 3) was considered as a novel anti tumor agent However, it showed severe toxicity effect on normal tissue at the same time To improve its therapeutic efficacy and de...Background Recently, arsenic trioxide (As 2O 3) was considered as a novel anti tumor agent However, it showed severe toxicity effect on normal tissue at the same time To improve its therapeutic efficacy and decrease its toxicity,we prepared arsenic trioxide loaded albuminutes immuno nanospheres [As 2O 3 (HAS NS) BDI 1] targeted with nonoclonal antibody (McAb) BDI 1 and tested its specific killing effect against bladder cancer cell Methods As 2O 3 HAS NS was prepared by chemical cross linking method Monoclonal antibody BDI 1 was purified with ammonium sulphate saltingout and chromatography Albuminutes microspheres were conjugated with McAb by SPDP cross linking method Concentration of As in As 2O 3 (HAS NS) BDI 1 and As 2O 3 HAS NS was measured by atomic fluometry method As 2O 3 (HAS NS) BDI 1 and its activity were detected by SDS PAGE reduction electrophoresis, indirect immunofluorescence test, light microscope and scanning electron microscope observation Acridine orange staining and tritiated thymidine ( 3H TdR) incorporation tests were used to indicate specific killing activity of As 2O 3 (HAS NS) BDI 1 in vitro Results In As 2O 3 (HAS NS) BDI 1 groups, we saw two protein bands in SDS PAGE reduction electrophoresis Albuminutes immuno nanospheres were rounded with clear green fluorescence by immunofluorescence test Under microscope, we observed that BIU 87 cells were covered with the As 2O 3 (HAS NS) BDI 1 and that As 2O 3 (HAS NS) BDI 1 moved with the BIU 87 cells The albuminutes immuno nanospheres were tightly junctioned with the BIU 87 cells Specific killing activity of As 2O 3 (HAS NS) BDI 1 on bladder tumor cells was observed by acridine orange staining and 3H TdR incorporation assays Conclusions As 2O 3 (HAS NS) BDI 1 might bind specifically against BIU 87 cells, thus leading to high activity of killing bladder tumor cells展开更多
In this paper, we propose an approach to solve the power control issue in a Digital Cellular System( DS CDMA ) cellular system using Genetic Algorithms ( GAs ). The transmitter power control developed in this pap...In this paper, we propose an approach to solve the power control issue in a Digital Cellular System( DS CDMA ) cellular system using Genetic Algorithms ( GAs ). The transmitter power control developed in this paper has been proven to be efficient to control co channel interference, to increase bandwidth utilization and to balance the comprehensive services that are shared among all the mobiles with attaining a common Signal to Interference Ratio ( SIR ). In this paper, the optimal Centralized Power Control( CPC ) vector is characterized and its optimal solution to CPC is presented using GAs , in which First In First Out ( FIFO ) stacks and non linear decreasing functions are derived in the investigation for enforceing the convergence rate. Emphasis is put on the balance of services and convergence rate by using GAs .展开更多
A new method is presented in this paper for fitting Voltage-to-Frequency Converter (VFC's) output functions by using Functionally Connected High-order Neural Networks (FCHNN). The nonlinear estimation is implement...A new method is presented in this paper for fitting Voltage-to-Frequency Converter (VFC's) output functions by using Functionally Connected High-order Neural Networks (FCHNN). The nonlinear estimation is implemented when the VFC110 is used at a full-scale output frequency of 4 MHz. Two kinds of on-line dynamic calibrating circuits are designed to improve the sampling precision. This method can also be applied to different industrial areas.展开更多
Next generation wireless communication is based on a global system of fixed and wireless mobile services thatare transportable across different network back-bones,network service providers and network geographical bou...Next generation wireless communication is based on a global system of fixed and wireless mobile services thatare transportable across different network back-bones,network service providers and network geographical boundaries.This paper presents an approach to investigate the effects of soft handover and perfect power control on the forward link ina DS-CDMA cellular system.Especially,the relationships between the size ofhandover zone and the capacity gain are e-valuated under the log-normal shadow channel.Then the optimization of maximum forward capacity is very necessary tobe done with the maximum size of soft handover zone to the various system characteristics.展开更多
文摘Heavy metal contamination of soil has become a major environmental concern worldwide over the past several decades,the exploitation and application of heavy metal hyperaccumulating plants will defend the safety of food and environment on which human life relied,whereas using natural hyperaccumulators is insufficient,due
文摘Aim: To study the expression of MMP1 and TIMP1 in normal and radiation-combined wound healing and their effects on the healing process. Materials and Methods: A rat model of radiation-combined wound healing was used, and routine light microscopy, electron microscopy, immunohistochemistry, and in situ hybridization were used to detect the expression of MMP1 and TIMP1 during the healing process. Results: The wound healing process was impaired and delayed. In rats receiving 25Gy Gamma ray locally, the irradiated wounds healed 6 days later than non-irradiated controls. The following changes were found in the expression of MMP1 and TIMP1: (1) In the early inflammatory phase and in the period of granulation tissue formation, MMP1 expression was only slightly if at all affected in the newly-formed epidermis of irradiated wounds when compared with that in controls. Later, the epidermal expression of MMP1 in irradiated wounds was comparatively increased following the delayed healing process. 3 to 14 days after wounding, TIMP1 was weakly positive in proliferating keratinocytes of control group and became negative after epidermal covering, whereas no or only slight epidermal expression was detected in the irradiated group before epidermal covering. (2) The expression of MMP1 and TIMP1 in irradiated group was markedly decreased in fibroblasts, endotheliocytes and macrophages when compared to that in controls. The expression phase was prolonged due to the delayed healing process. Conclusions: It is concluded that the reduced expression of MMP1 and TIMP1 in granulation tissue retards such important processes as cell migration and angiogenesis, thus slowing the healing process. The expression of MMP1 in the newly-formed epidermis may help the process of reepithelialization, but in the late healing period, overexpression of MMP1 and decreased expression of TIMP1 in the epidermis may hinder the establishment of basal membrane and scar formation.
基金ThisstudywassupportedbyNationalYouthNatureScienceFoundationofChina (No 3 0 2 0 0 2 84)
文摘Background Recently, arsenic trioxide (As 2O 3) was considered as a novel anti tumor agent However, it showed severe toxicity effect on normal tissue at the same time To improve its therapeutic efficacy and decrease its toxicity,we prepared arsenic trioxide loaded albuminutes immuno nanospheres [As 2O 3 (HAS NS) BDI 1] targeted with nonoclonal antibody (McAb) BDI 1 and tested its specific killing effect against bladder cancer cell Methods As 2O 3 HAS NS was prepared by chemical cross linking method Monoclonal antibody BDI 1 was purified with ammonium sulphate saltingout and chromatography Albuminutes microspheres were conjugated with McAb by SPDP cross linking method Concentration of As in As 2O 3 (HAS NS) BDI 1 and As 2O 3 HAS NS was measured by atomic fluometry method As 2O 3 (HAS NS) BDI 1 and its activity were detected by SDS PAGE reduction electrophoresis, indirect immunofluorescence test, light microscope and scanning electron microscope observation Acridine orange staining and tritiated thymidine ( 3H TdR) incorporation tests were used to indicate specific killing activity of As 2O 3 (HAS NS) BDI 1 in vitro Results In As 2O 3 (HAS NS) BDI 1 groups, we saw two protein bands in SDS PAGE reduction electrophoresis Albuminutes immuno nanospheres were rounded with clear green fluorescence by immunofluorescence test Under microscope, we observed that BIU 87 cells were covered with the As 2O 3 (HAS NS) BDI 1 and that As 2O 3 (HAS NS) BDI 1 moved with the BIU 87 cells The albuminutes immuno nanospheres were tightly junctioned with the BIU 87 cells Specific killing activity of As 2O 3 (HAS NS) BDI 1 on bladder tumor cells was observed by acridine orange staining and 3H TdR incorporation assays Conclusions As 2O 3 (HAS NS) BDI 1 might bind specifically against BIU 87 cells, thus leading to high activity of killing bladder tumor cells
文摘In this paper, we propose an approach to solve the power control issue in a Digital Cellular System( DS CDMA ) cellular system using Genetic Algorithms ( GAs ). The transmitter power control developed in this paper has been proven to be efficient to control co channel interference, to increase bandwidth utilization and to balance the comprehensive services that are shared among all the mobiles with attaining a common Signal to Interference Ratio ( SIR ). In this paper, the optimal Centralized Power Control( CPC ) vector is characterized and its optimal solution to CPC is presented using GAs , in which First In First Out ( FIFO ) stacks and non linear decreasing functions are derived in the investigation for enforceing the convergence rate. Emphasis is put on the balance of services and convergence rate by using GAs .
文摘A new method is presented in this paper for fitting Voltage-to-Frequency Converter (VFC's) output functions by using Functionally Connected High-order Neural Networks (FCHNN). The nonlinear estimation is implemented when the VFC110 is used at a full-scale output frequency of 4 MHz. Two kinds of on-line dynamic calibrating circuits are designed to improve the sampling precision. This method can also be applied to different industrial areas.
文摘Next generation wireless communication is based on a global system of fixed and wireless mobile services thatare transportable across different network back-bones,network service providers and network geographical boundaries.This paper presents an approach to investigate the effects of soft handover and perfect power control on the forward link ina DS-CDMA cellular system.Especially,the relationships between the size ofhandover zone and the capacity gain are e-valuated under the log-normal shadow channel.Then the optimization of maximum forward capacity is very necessary tobe done with the maximum size of soft handover zone to the various system characteristics.
