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血小板与肿瘤物质交换的研究进展 被引量:1
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作者 管俊锋 佘思齐 +3 位作者 解千歆 胡佳音 朱炳祺 胡梦娇 《中国病理生理杂志》 CAS CSCD 北大核心 2023年第5期910-918,共9页
血小板是由巨核细胞产生的无核细胞,可通过活化、聚集、粘附和释放等反应参与生理性止血以及维持血管完整性,且在血栓形成、动脉粥样硬化和免疫反应等病理过程中发挥关键作用[1]。血小板富含多种生物活性物质,如细胞因子(生长因子、趋... 血小板是由巨核细胞产生的无核细胞,可通过活化、聚集、粘附和释放等反应参与生理性止血以及维持血管完整性,且在血栓形成、动脉粥样硬化和免疫反应等病理过程中发挥关键作用[1]。血小板富含多种生物活性物质,如细胞因子(生长因子、趋化因子、转化生长因子等),小分子核酸[非编码RNA如微小RNA(microRNA, miRNA)等],活化的血小板通过释放的生物活性物质参与各种生理病理过程。 展开更多
关键词 血小板 肿瘤 细胞外囊泡 物质交换
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连续流动注射分光光度法测定杏仁中氰化物和挥发酚的含量 被引量:1
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作者 俞佳铭 沙贤亮 +2 位作者 朱炳祺 秦延平 史一丹 《理化检验(化学分册)》 CAS CSCD 北大核心 2023年第6期678-682,共5页
提出了连续流动注射分光光度法同时测定杏仁中氰化物和挥发酚含量的方法。2.00 g杏仁样品经25 mL 0.05 mol·L^(-1)硫酸溶液涡旋1 min,浸泡10 min,水解释放样品中氰苷后,用2 g·L^(-1)氢氧化钠溶液固定待测物,并定容至50 mL。... 提出了连续流动注射分光光度法同时测定杏仁中氰化物和挥发酚含量的方法。2.00 g杏仁样品经25 mL 0.05 mol·L^(-1)硫酸溶液涡旋1 min,浸泡10 min,水解释放样品中氰苷后,用2 g·L^(-1)氢氧化钠溶液固定待测物,并定容至50 mL。取上清液,经0.45μm滤膜过滤,滤液在优化的试验条件下采用连续流动注射分光光度法测定其中氰化物和挥发酚的含量。结果表明,氰化物和挥发酚的质量浓度在0.002~0.200 mg·L^(-1)内与对应的峰高呈线性关系,检出限分别为0.005 mg·kg^(-1)和0.0175 mg·kg^(-1)。取3份样品,每个样品重复测定6次,测定值的相对标准偏差(n=6)均小于2.0%。按照标准加入法对实际样品进行回收试验,回收率为97.6%~105%。方法用于分析10份杏仁样品,氰化物的检出量为10.6~56.4 mg·kg^(-1),平均值为32.3 mg·kg^(-1),挥发酚的检出量为14.7~54.8 mg·kg^(-1),平均值为31.9 mg·kg^(-1)。方法利用硫酸溶液水解样品中氰苷后用碱溶液固定,解决了碱溶液直接提取结果偏低的问题。 展开更多
关键词 连续流动注射分光光度法 氰化物 挥发酚 杏仁
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综合管廊智能运维关键影响因素分析 被引量:6
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作者 王婉 张向先 +1 位作者 诸秉奇 滕佳颖 《实验室研究与探索》 CAS 北大核心 2020年第11期30-34,共5页
以综合管廊智能运维为研究对象,贝叶斯网络为主要手段构建了综合管廊内部环境智能运维的指标体系,并在此基础上明确了综合管廊内部环境智能运维的18个关键影响因素。发现当危险敏感值增加时,会增加通信系统损坏、供电设备损坏与风管、... 以综合管廊智能运维为研究对象,贝叶斯网络为主要手段构建了综合管廊内部环境智能运维的指标体系,并在此基础上明确了综合管廊内部环境智能运维的18个关键影响因素。发现当危险敏感值增加时,会增加通信系统损坏、供电设备损坏与风管、风道系统这3个潜在敏感性因素,应予以重点关注,以提升综合管廊智能运维的风险预警能力。 展开更多
关键词 综合管廊 贝叶斯网络 安全运维
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数据挖掘视角下原发性视网膜色素变性的中医用药规律分析 被引量:1
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作者 徐伟 胡雨涛 +3 位作者 叶欣 谢珺 朱炳祺 胡梦娇 《临床合理用药杂志》 2022年第19期29-32,共4页
目的通过大数据挖掘的视角,分析中医对原发性视网膜色素变性(RP)的用药思路、配伍规律,以拓展对该病的治疗方法。方法利用网络资料库选取符合标准的文献,运用Excel 2015对中药的使用频次进行统计和计算,以频次≥10为标准,筛选高频中药。... 目的通过大数据挖掘的视角,分析中医对原发性视网膜色素变性(RP)的用药思路、配伍规律,以拓展对该病的治疗方法。方法利用网络资料库选取符合标准的文献,运用Excel 2015对中药的使用频次进行统计和计算,以频次≥10为标准,筛选高频中药。用Excel 2015对高频中药的性味归经进行统计分析,使用SPSS 26.0对高频中药进行加权聚类分析和t检验。结果初检文献307篇,后纳入文献37篇,单个中药使用频率≥10的共10味,分别为当归、菟丝子、枸杞子、丹参、黄芪、熟地黄、山药、茺蔚子、川芎、茯苓,药性以温、平居多;五味多为甘、辛;归经以肝、肾、脾为主;功效主要为活血调经、养心安神。聚类分析结果有3个聚类群,发现黄芪、茯苓、川芎、山药等中药配伍“功能模块”。结论数据挖掘可发现治疗RP的中药配伍规律,即在中药治疗RP的过程中,应以甘味、温性药为主,肝肾脾同治,再根据患者的实际情况,辨证加减施治,可与针灸、西药等方法结合治疗,同时为新处方的开发提供思路。 展开更多
关键词 原发性视网膜色素变性 数据挖掘 中药 用药规律
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改进的QuEChERS方法结合在线凝胶色谱-气相色谱-串联质谱法测定茶叶中39种有机磷农药的含量 被引量:13
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作者 朱炳祺 金绍强 +4 位作者 徐潇颖 胡帆 田春霞 张淼 罗金文 《理化检验(化学分册)》 CAS CSCD 北大核心 2018年第4期433-442,共10页
将茶叶样品4.000 0g、乙腈10.0mL和4.00mg·L^(-1)环氧七氯内标溶液400μL涡漩混合1min,超声提取15min,离心后取上清液1.0mL,加入甲苯1mL,经含N-丙基乙二胺(PSA)30mg、C1830mg、石墨化炭黑(GCB)15mg和无水硫酸镁150mg的分散固相萃... 将茶叶样品4.000 0g、乙腈10.0mL和4.00mg·L^(-1)环氧七氯内标溶液400μL涡漩混合1min,超声提取15min,离心后取上清液1.0mL,加入甲苯1mL,经含N-丙基乙二胺(PSA)30mg、C1830mg、石墨化炭黑(GCB)15mg和无水硫酸镁150mg的分散固相萃取吸附剂净化后,采用在线凝胶色谱-气相色谱-串联质谱法(Online GPC-GC-MS/MS)测定净化溶液中39种有机磷农药残留量。结果表明:39种有机磷农药在一定范围内呈线性关系,检出限(3S/N)为0.5~4.3μg·kg^(-1)。按标准加入法进行回收试验,回收率为83.1%~105%,相对标准偏差(n=6)均小于9.0%。 展开更多
关键词 QUECHERS 在线凝胶色谱-气相色谱-串联质谱法 有机磷农药 茶叶
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QuEChERS提取-高效液相色谱-串联质谱法测定蔬菜中29种除草剂的残留量 被引量:10
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作者 徐潇颖 刘柱 +3 位作者 朱炳祺 梁晶晶 陈万勤 罗金文 《理化检验(化学分册)》 CAS CSCD 北大核心 2019年第4期420-426,共7页
取匀浆后的蔬菜样品5.000g,加入甲酸-乙腈(1+99)混合液10.0 mL,振荡提取20min,加入氯化钠2.0g,继续振荡20min,离心5min。分取上清液1.5mL,加入由N-丙基乙二胺100mg、十八烷基硅烷100 mg和自制的磁化碳纳米管10 mg混合组成的净化剂进行Qu... 取匀浆后的蔬菜样品5.000g,加入甲酸-乙腈(1+99)混合液10.0 mL,振荡提取20min,加入氯化钠2.0g,继续振荡20min,离心5min。分取上清液1.5mL,加入由N-丙基乙二胺100mg、十八烷基硅烷100 mg和自制的磁化碳纳米管10 mg混合组成的净化剂进行QuEChERS提取净化,混合物经0.22μm滤膜过滤。滤液供高效液相色谱-串联质谱法分析。色谱分离中,选用Waters Cortects C18色谱柱为固定相,以不同比例组成的乙腈(A)和5mmol·L-1乙酸铵溶液(B)的混合液为流动相进行梯度洗脱。洗脱液中29种除草剂的残留量按质谱条件进行测定。结果表明:29种除草剂的质量浓度均在5.0~40μg·L-1范围内与对应的峰面积呈线性关系,检出限(3S/N)在0.5~1.5μg·kg-1之间。以空白蔬菜样品为基体,用标准加入法进行回收试验,测得29种化合物的回收率在83.9%~99.7%之间,测定值的相对标准偏差(n=6)在1.3%~5.6%之间。 展开更多
关键词 高效液相色谱-串联质谱法 QUECHERS 除草剂 蔬菜
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气相色谱-质谱联用法测定婴幼儿奶粉中37种脂肪酸和12种反式脂肪酸 被引量:7
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作者 田春霞 王远远 +2 位作者 吴苏妙 朱炳祺 金绍强 《理化检验(化学分册)》 CAS CSCD 北大核心 2020年第6期669-675,共7页
称取5.0g婴幼儿奶粉样品,在乙醇2mL,水4mL和氨水20mL的介质中,于75℃的恒温水浴中振荡水解40min,每间隔5min振荡一次,水解完成后冷却至室温,并在水解混合物中加入乙醇10mL,混匀。用体积比为1∶1的乙醚-石油醚混合液30mL振荡提取10min,离... 称取5.0g婴幼儿奶粉样品,在乙醇2mL,水4mL和氨水20mL的介质中,于75℃的恒温水浴中振荡水解40min,每间隔5min振荡一次,水解完成后冷却至室温,并在水解混合物中加入乙醇10mL,混匀。用体积比为1∶1的乙醚-石油醚混合液30mL振荡提取10min,离心5min,收集醚层提取液,留存于烧瓶中;再用乙醚-石油醚按相同步骤重复提取2次。将所收集的醚层提取液合并旋转蒸发至干,所得残留物即为从样品提取所得脂肪总量。取此提取所得脂肪60.0mg,溶解于异辛烷4mL中,再加入2mol·L^-1氢氧化钾-甲醇溶液200μL,振荡30s,静置至澄清,使脂肪酸甲酯化。加入硫酸氢钠1g中和氢氧化钾,然后离心3min。取其上层溶液按仪器工作条件进行气相色谱-质谱分析。采用高极性HP-88毛细管色谱柱,进样量为1.0μL,从130~240℃按程序升温的条件进行分离,并引入质谱仪进行测定,质谱测定中用电子轰击(EI)离子源,和在m/z 45~450内全扫描模式进行定性和定量测定。试验中选择样品中含量较低的反式脂肪酸为分析物,并测定了相关的参数。所测定的12种反式脂肪酸甲酯分别在一定的质量浓度内与其相应的峰面积之间呈线性关系,其检出限(3S/N)为0.42~1.45mg·L^-1。按标准加入法测得的回收率为75.5%~107%,测定值的相对标准偏差(n=5)在1.1%~8.5%之间。脂肪酸采用面积归一化法测定。按此方法分析了3种婴儿奶粉,其中饱和脂肪酸含量在50%以上,反式脂肪酸含量均小于0.5%,均符合国家标准规定。 展开更多
关键词 气相色谱-质谱法 脂肪酸 反式脂肪酸 婴幼儿奶粉
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气相色谱-三重四极杆质谱法测定食用香精中3-乙酰基-2,5-二甲基噻吩 被引量:4
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作者 茅佩卿 金绍强 +3 位作者 王展华 朱炳祺 徐潇颖 罗金文 《理化检验(化学分册)》 CAS CSCD 北大核心 2019年第3期328-332,共5页
称取约0.1g样品,加入约6mL正庚烷,超声提取15min,静置冷却后用正庚烷定容至10.0mL,混匀后静置数分钟,取上清液过0.22μm有机相滤膜后,采用Agilent HP-INNOWax毛细管柱(30m×0.25mm,0.25μm)进行分离,质谱中选择电子轰击离子源和多... 称取约0.1g样品,加入约6mL正庚烷,超声提取15min,静置冷却后用正庚烷定容至10.0mL,混匀后静置数分钟,取上清液过0.22μm有机相滤膜后,采用Agilent HP-INNOWax毛细管柱(30m×0.25mm,0.25μm)进行分离,质谱中选择电子轰击离子源和多反应监测模式。3-乙酰基-2,5-二甲基噻吩的质量浓度在0.010~2.00 mg·L^(-1)内与其峰面积呈线性关系,检出限(3S/N)为0.1mg·kg^(-1)。以稀膏状香精样品为基质进行加标回收试验,3-乙酰基-2,5-二甲基噻吩的回收率在82.7%~92.9%之间,测定值的相对标准偏差(n=6)在0.70%~2.8%之间。以不同类型的香精样品为基质进行加标回收试验,回收率在92.2%~107%之间。 展开更多
关键词 气相色谱-三重四极杆质谱法 3-乙酰基-2 5-二甲基噻吩 食用香精 超声提取
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岩石物理风化的研究进展与问题概述
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作者 朱秉启 孙晨 张嘉欣 《中国沙漠》 CSCD 北大核心 2023年第2期281-287,共7页
简要回顾了学术界近期关于地表岩石物理风化的理论研究进展、认识和存在的问题,针对“亚临界裂纹”理论、“气候驱动”与亚临界裂纹的发育、岩石风化的影响因素——单因素作用与综合效应(本质性与普遍性)、行星尺度的物理风化——撞击... 简要回顾了学术界近期关于地表岩石物理风化的理论研究进展、认识和存在的问题,针对“亚临界裂纹”理论、“气候驱动”与亚临界裂纹的发育、岩石风化的影响因素——单因素作用与综合效应(本质性与普遍性)、行星尺度的物理风化——撞击驱动或热循环驱动等问题逐一进行了阐述和评论,并为下一步的研究提出新的建议。 展开更多
关键词 物理风化/机械风化 岩石破坏 亚临界裂纹 气候驱动/气候依赖性 热疲劳/热循环
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QuEChERS-高效液相色谱-串联质谱法测定春笋中4种农药残留 被引量:14
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作者 周霞 陈万勤 +3 位作者 陈晶燕 朱雅青 朱炳祺 罗金文 《中国食品卫生杂志》 北大核心 2018年第5期486-490,共5页
目的建立检测春笋中吡虫啉、茚虫威、辛硫磷和克百威4种农药的QuEChERS-高效液相色谱-串联质谱法。方法前处理采用QuEChERS方法,乙腈为提取液,N-丙基乙二胺(PSA)为净化剂,结合液相色谱-质谱联用技术,正离子多反应监测(MRM)模式进行检测... 目的建立检测春笋中吡虫啉、茚虫威、辛硫磷和克百威4种农药的QuEChERS-高效液相色谱-串联质谱法。方法前处理采用QuEChERS方法,乙腈为提取液,N-丙基乙二胺(PSA)为净化剂,结合液相色谱-质谱联用技术,正离子多反应监测(MRM)模式进行检测,外标法定量。结果吡虫啉、茚虫威、辛硫磷和克百威4种农药在0.5~20.0 ng/ml范围呈良好的线性关系,相关系数(r^2)均大于0.991,在4~40μg/kg加标范围内,4种农药的回收率为72.4%~92.8%,精密度为1.5%~9.2%,方法的检出限为0.4μg/kg,定量限为1.2μg/kg。结论建立了适用于检测春笋中吡虫啉、茚虫威、辛硫磷和克百威4种农药的QuEChERS-高效液相色谱-串联质谱法,该方法准确、灵敏、高效、简便。 展开更多
关键词 QUECHERS 高效液相色谱-串联质谱 春笋 农药残留 吡虫啉 茚虫威 辛硫磷 克百威 测定
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Formation and evolution of sand deserts in Xinjiang, Northwest China: I. Provenances of desert sands 被引量:3
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作者 zhu bingqi YU Jingjie +3 位作者 QIN Xiaoguang Patrick RIOUAL LIU Ziting XIONG Heigang 《Journal of Geographical Sciences》 SCIE CSCD 2014年第1期177-190,共14页
Sandy desert landscape is a geological product of arid climate and abundant sand materials supply in natural conditions, therefore the formation of sandy desert is an ideal studying object for understanding the intera... Sandy desert landscape is a geological product of arid climate and abundant sand materials supply in natural conditions, therefore the formation of sandy desert is an ideal studying object for understanding the interaction between various stratigraphic/epigenetic spheres of the earth system. However, until now, the knowledge about the provenance and formation of these deserts in Xinjiang, Central Asia is diverse and inconsistent, a systematic review is necessary. Ancient aeolian sand sediments in the internal areas of these deserts and their twin dust deposits in the Cenozoic strata surrounding the Xinjiang deserts are direct and indirect clues reflecting the provenance of sand materials and the formation and evolution of deserts. Based on the geochemical, mineralogical and isotopic evidences of desert sands and relevant deposits in the peripheral regions, this paper reviews the research progress on the development of the sandy deserts in Xinjiang, Northwest China. Many proofs proposed that desert sands in Xinjiang were mainly sourced from the ancient pluvial, alluvial and fluvial sediments and were eventually achieved because of the local sand supply. It pointed out that the settings of tectonic structure in Xinjiang had made sediments both in the Tarim Basin and the Junggar Basin being influenced greatly by regional hydrological system and aeolian processes originated from the planetary wind system of westerly, the East Asian winter monsoon and the topographical mountain-valley winds. However, the directions of transpor- tation paths are different between the hydrological dynamics and the aeolian dynamics, which are decided by the slantwise structures of the basins tectonics and the regional atmospheric circulation routes, respectively, and have determined anisotropies of the transport pathway of these sediments delivered by different agents. This results in a geological cycle of detrital sediment transportation in the two large sediment mixing, alternation and recycle basins and thus largely increases the degree of between younger and older sediment sources, as well as the complexity and diversity of sand provenances. 展开更多
关键词 sandy desert PROVENANCE hydrodynamic and aeoliandynamic agents sediment transportation XINJIANG
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Formation and evolution of sand deserts in Xinjiang, Northwest China:II. The palaeo-environmental reconstruction 被引量:1
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作者 zhu bingqi YU Jingjie +3 位作者 QIN Xiaoguang Patrick RIOUAL ZHANG Yichi XIONG Heigang 《Journal of Geographical Sciences》 SCIE CSCD 2014年第3期539-559,共21页
Based on the palaeoclimatic and palaeoenvironmental evidences of geological history and human history periods, this paper reviews the researches and progresses on the development of the sandy deserts in Xinjiang. It p... Based on the palaeoclimatic and palaeoenvironmental evidences of geological history and human history periods, this paper reviews the researches and progresses on the development of the sandy deserts in Xinjiang. It pointed out that the features of tectonic structure in Xinjiang had made both the Tarim Basin and the Junggar Basin being influenced greatly by the foehn effects originated from the planetary wind system of westerly, the East Asian ocean-continental monsoon and the topographical mountain-valley winds. The regional patterns of climate and environment since the Quaternary were characterized by the overall persistent drought accompanied by fluctuations in the secondary scale. Formations of aeolian sediments in the basins and at the margins are a potential response to global climate change, particularly the aridification of the Asian hinterland deduced by the uprising of the Tibetan Plateau and its surrounding highlands. For the question about the formation time of the Tak- lamakan Desert, because the research methods, objects and information carriers used in previous studies are different, there are many disputes in the academic circles at present. Evidences from aeolian deposits/rocks at the edge and in the hinterland of these sandy de- serts and their chronological data indicate that an arid climate and land surface aeolian processes have occurred at the edge of the Tarim Basin and its hinterland areas since the Tertiary period. However, the duration time of these processes at mass scale should have begun after the middle Pleistocene and lasted to the Holocene. Occurrence of dune fields in recent 2000 years in the oasis areas should be greatly influenced by human factors. 展开更多
关键词 sandy desert TERTIARY QUATERNARY ancient aeolian sediment palaeoclimate change XINJIANG
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The recent evolution of dune landforms and its environmental indications in the mid-latitude desert area(Hexi Corridor)
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作者 zhu bingqi 《Journal of Geographical Sciences》 SCIE CSCD 2022年第4期617-644,共28页
The history of dune landform changes and dust activity at mid-latitudes is a good archive for exploring environmental changes and related landscape response.In this study,the dynamic changes,material sources,dust acti... The history of dune landform changes and dust activity at mid-latitudes is a good archive for exploring environmental changes and related landscape response.In this study,the dynamic changes,material sources,dust activity history and the influencing factors of typical sand dunes in the Hexi Corridor were comprehensively analyzed from the aspects of aeolian geomorphology,grain-size sedimentology,geochemistry and climatology.The results show that in the past half century,the typical crescent-shaped dunes and chains of crescent-shaped dunes in the study area have moved or swayed greatly,with an average speed ranging from 0.8 m/a(Dunhuang)to 6.2 m/a(Minqin).The dynamic changes of sand dunes are mainly affected by annual precipitation,annual average wind speed and annual gale days,which indicates that climate is the primary influencing factor of dune landform changes.The three-stage grain-size curve model of dune sands is obviously different from that of gobi sediments(two-stage),revealing the“immaturity”of the latter in sedimentology,while the former has experienced efficiently aeolian differentiation and non-local origin.The comprehensive evidences of paleogeography,sedimentology and geochemistry reveal that the source materials of sand dunes are mainly alluvial/proluvial and palaeo-fluvial sediments,including clastic sediments in the denudation/erosion zones of the north and south piedmonts.Indicators such as the proportion of surface fine particles,the coverage of surface salt crusts,and the content of erodible sandy materials indicate that the western gobi areas are not the main source areas of wind-blown dust in the central and eastern parts of the Hexi Corridor.The spatial distribution of the movement direction of sand dunes is similar to that of the regional dominant wind direction,which indicates that the difference in the dynamic evolution of dune landforms between the east and west of the Hexi Corridor should be controlled by the regional-scale wind system,that is,controlled by the dynamic mechanism rather than the difference in material sources.The warming and humidification of the Hexi climate is a synchronous response to the global warming and the strengthening of the Asian Summer Monsoon.It is also the main reason for the reduction of dust storms in the study area,which means that a potential inverse desertification process exists in the Hexi Corridor during the same period and it is also controlled by climate change.However,the process of desertification in the oasis areas during the period is caused by groundwater fluctuation affected by human activities. 展开更多
关键词 dune landform Gobi landform grain size sedimentology elemental geochemistry global warming DESERTIFICATION mid-latitude deserts
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