Salmonella Enteritidis(SE)is a zoonotic and vertically transmitted pathogen,often colonized in the reproductive tract of adult poultry,which can result in direct contamination of eggs and threaten human health.Previou...Salmonella Enteritidis(SE)is a zoonotic and vertically transmitted pathogen,often colonized in the reproductive tract of adult poultry,which can result in direct contamination of eggs and threaten human health.Previous studies have revealed that some pattern recognition receptors and resistance genes were involved in regulating immune responses to SE invasion in birds.However,the role of these immune response genes was not independent,and the interactions among the genes remained to be further investigated.In this study,SE burden and colonization were determined in reproductive tissue after the ducks were SE-infected,and RNA-sequencing was performed to construct co-expression networks by weighted gene co-expression network analysis(WGCNA).The result showed that SE could be isolated from 22% of infected-birds in any segment of the reproductive tract and the SE was readily colonized in the stroma,small follicle,isthmus,and vagina of the reproductive tracts in morbid ducks.The top central,highly connected genes were subsequently identified three specific modules in the above four tissues at the defined cut-offs(P<0.01),including 60 new candidate regulators and 125 transcription factors.Moreover,those 185 differentially expressed genes(DEGs)in these modules were co-expressed.Moreover,the hub genes(TRAF3,CXCR4 and IL13RA1)were identified to act with many other genes through immune response pathways including NF-kappaB,Toll-like receptor,steroid biosynthesis,and p53signaling pathways.These data provide references that will understand the immune regulatory relationships during SE infection,but also assist in the breeding of SE-resistant lines through potential biomarkers.展开更多
The load transfer characteristics of a tensile anchor in the rock mass with weak interlayers were investigated,considering the nonuniform stress of the horizontally layered rock mass along anchors.An improved shear-sl...The load transfer characteristics of a tensile anchor in the rock mass with weak interlayers were investigated,considering the nonuniform stress of the horizontally layered rock mass along anchors.An improved shear-slipping model was proposed to describe the stress evolution characteristics of the bolt-rock interface.Based on the improved model,analytical solutions of the axial force,shear stress distribution and load-displacement relationship considering the residual stress stage were established.The effects of the stratigraphic sequence,pulling force and bolt diameter on the stress distribution of the anchorage interface were evaluated by using analytical solutions.The results were verified by applying the finite difference numerical simulation method.The sensitivity of each parameter to the axial force and shear stress of the rock bolt was determined based on calculation of the sensitivity coefficient.The study results show that the axial force and shear stress tend to decrease nonuniformly along the rock bolt towards the anchorage depth.Due to the existence of weak interlayers,the shear stress mutates at the weak and hard rock interface,and the axial force appears to“rebound”at the bottom of the anchored section.Lithology has more significant effects on the axial force and shear stress at the bottom of the anchor than at the top of the anchor.The pulling force is more sensitive to the anchor stress than stratigraphic sequence when the bolt diameter is determined.This study provides a theoretical framework for the fundamental problem of tensile bolts in horizontally or vertically laminated rock masses,providing a theoretical basis for anchor design.展开更多
基金supported by the National Natural Science Foundation of China(31702107)the Open Project Program of Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China,Yangzhou University,China(JILARKF202018)。
文摘Salmonella Enteritidis(SE)is a zoonotic and vertically transmitted pathogen,often colonized in the reproductive tract of adult poultry,which can result in direct contamination of eggs and threaten human health.Previous studies have revealed that some pattern recognition receptors and resistance genes were involved in regulating immune responses to SE invasion in birds.However,the role of these immune response genes was not independent,and the interactions among the genes remained to be further investigated.In this study,SE burden and colonization were determined in reproductive tissue after the ducks were SE-infected,and RNA-sequencing was performed to construct co-expression networks by weighted gene co-expression network analysis(WGCNA).The result showed that SE could be isolated from 22% of infected-birds in any segment of the reproductive tract and the SE was readily colonized in the stroma,small follicle,isthmus,and vagina of the reproductive tracts in morbid ducks.The top central,highly connected genes were subsequently identified three specific modules in the above four tissues at the defined cut-offs(P<0.01),including 60 new candidate regulators and 125 transcription factors.Moreover,those 185 differentially expressed genes(DEGs)in these modules were co-expressed.Moreover,the hub genes(TRAF3,CXCR4 and IL13RA1)were identified to act with many other genes through immune response pathways including NF-kappaB,Toll-like receptor,steroid biosynthesis,and p53signaling pathways.These data provide references that will understand the immune regulatory relationships during SE infection,but also assist in the breeding of SE-resistant lines through potential biomarkers.
基金supported by the National Key R&D Program of China(Nos.2018YFC1505300,2017YFC1501304)National Natural Science Foundation of China(Grout Nos.42090054,41922055 and 41931295)+2 种基金the Fundamental Research Funds for the Central Universities,China University of Geosciences(Wuhan)(No.CUGGC09)Research Project of China Three Gorges Corporation(No.2019073)the Zhejiang Huadong Construction Engineering Co.,Ltd.(No.KY2019-HDJS-07)。
文摘The load transfer characteristics of a tensile anchor in the rock mass with weak interlayers were investigated,considering the nonuniform stress of the horizontally layered rock mass along anchors.An improved shear-slipping model was proposed to describe the stress evolution characteristics of the bolt-rock interface.Based on the improved model,analytical solutions of the axial force,shear stress distribution and load-displacement relationship considering the residual stress stage were established.The effects of the stratigraphic sequence,pulling force and bolt diameter on the stress distribution of the anchorage interface were evaluated by using analytical solutions.The results were verified by applying the finite difference numerical simulation method.The sensitivity of each parameter to the axial force and shear stress of the rock bolt was determined based on calculation of the sensitivity coefficient.The study results show that the axial force and shear stress tend to decrease nonuniformly along the rock bolt towards the anchorage depth.Due to the existence of weak interlayers,the shear stress mutates at the weak and hard rock interface,and the axial force appears to“rebound”at the bottom of the anchored section.Lithology has more significant effects on the axial force and shear stress at the bottom of the anchor than at the top of the anchor.The pulling force is more sensitive to the anchor stress than stratigraphic sequence when the bolt diameter is determined.This study provides a theoretical framework for the fundamental problem of tensile bolts in horizontally or vertically laminated rock masses,providing a theoretical basis for anchor design.
